A method for enzymatically assisted extraction of longan polysaccharide
A longan polysaccharide and auxiliary extraction technology, which is applied in the field of enzymatically assisted extraction of longan polysaccharides, can solve the problems that restrict the research and application of longan polysaccharide activity, and the research on the activity of longan polysaccharide is less, and achieve the effect of mild conditions, obvious effect and convenient operation
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Embodiment 1
[0020] Step 1. Enzyme fermentation: inoculate Pichia pastoris GS115 containing α-L-rhamnosidase gene into YPD medium for activation, then transfer to BMGY medium to continue activation, and collect OD 600 The cells were 2.0-3.0, and transferred to BMMY medium, placed at 30°C to induce expression; every 24 h, sterile methanol was added to induce expression for 7 days, and the enzyme solution was collected by centrifugation. The YPD medium, BMGY medium and BMMY medium are conventional medium for cultivating yeast.
[0021] Step 2. Preparation of enzyme preparation: set the spray pressure to 0.2 Mpa, the inlet air temperature to 135°C, the inlet air velocity to 4 m³ / min, the feed rate to 800 ml / h, and 100 mL of α-L-rhamnosidase 15 g of maltodextrin was added to the enzyme solution, and spray-dried to prepare α-L-rhamnosidase enzyme preparation.
Embodiment 2
[0023] Step 1. Enzyme treatment: Soak longan in 2 times water, add 1% α-L-rhamnosidase enzyme preparation, and warm bath at 50°C for 1.5 h;
[0024] Step 2, water extraction: the solution obtained in step 1 is subjected to water extraction twice, the first water addition is 8 times the quality of longan, and the water extraction time is 0.5 h; the second water addition is 5 times the quality of longan, water Lifting time is 0.5 h;
[0025] Step 3, Concentration and Alcohol Precipitation: Concentrate the longan polysaccharide water extract obtained in Step 2 by rotary evaporation, then add 3 times the volume of the concentrated solution in absolute ethanol, let it stand for 15 hours, then filter to obtain insoluble matter, and dry the insoluble matter Finally, the longan polysaccharide can be obtained, and the polysaccharide content is 2.78%.
[0026] Step 4, control group: Soak longan in 2 times the water and warm bath at 50°C for 1.5 h, then carry out the same water extracti...
Embodiment 3
[0029] Step 1. Enzyme treatment: Soak longan in 4 times water, add 2% α-L-rhamnosidase enzyme preparation, and warm bath at 60°C for 0.5 h;
[0030] Step 2, water extraction: the solution obtained in step 1 is subjected to water extraction twice, the first water addition is 5 times the mass of longan, and the water extraction time is 1 h; the second water addition is 1 time the mass of longan, water The lifting time is 1 h;
[0031] Step 3, Concentration and Alcohol Precipitation: Concentrate the longan polysaccharide water extract obtained in Step 2 by rotary evaporation, then add 3 times the volume of the concentrated solution in absolute ethanol, let it stand for 15 hours, then filter to obtain insoluble matter, and dry the insoluble matter Finally, the longan polysaccharide can be obtained, and the polysaccharide content is 3.07%.
[0032] Step 4, control group: Soak longan in 4 times of water and warm bath at 60°C for 0.5 h, then perform the same water extraction, concen...
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