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Cyclocarya paliurus tissue culture seedling rooting culture medium and rooting culture method

A rooting medium and rooting culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration and other directions, can solve the problems of difficulty in meeting the needs of factory seedling raising, low rooting rate, poor rooting quality, etc., to promote rooting and plant growth. Strong growth and good root development

Active Publication Date: 2016-12-07
GUILIN BOLIN BIOLOGICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing research results show that tissue culture seedlings of Cyclocarya paliurus can be successfully multiplied, but its rooting rate is low and rooting quality is poor, so it is difficult to meet the requirements of industrial seedling cultivation

Method used

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  • Cyclocarya paliurus tissue culture seedling rooting culture medium and rooting culture method
  • Cyclocarya paliurus tissue culture seedling rooting culture medium and rooting culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] 1) Get the current year's branches of Cyclocarya paliurus, and obtain tissue culture seedlings by in vitro culture according to conventional methods, such as figure 1 shown;

[0034] 2) Rooting culture: cut out a single tissue culture seedling that is higher than 3.5 cm and has 3 to 4 compound leaves, and insert it into the rooting medium for 35 days (the picture of the root of the rooted seedling after 35 days of culture is as follows: figure 2 shown); where:

[0035]The formula of the rooting medium is: improved MS+IBA 1.0 mg / L+NAA 0.5 mg / L+ diethyl hexanoate 0.05 mg / L+ sucrose 20 g / L+ agar 7 g / L, pH 5.8.

[0036] The rooting culture conditions are as follows: the temperature is 23-25°C, the light cycle is 12h / d, and the light intensity is 2000Lx.

[0037] Calculate the rooting rate (the same below) by the number of rooted strains / inoculated strains × 100%, and the rooting rate is 82%.

Embodiment 2

[0048] 1) Get the current year's branches of Cyclocarya paliurus, and obtain tissue culture seedlings by in vitro culture according to conventional methods;

[0049] 2) Rooting culture: cut out a single tissue culture seedling that is higher than 2.5 cm and has 2 to 3 compound leaves, and insert it into the rooting medium for 30 days; wherein:

[0050] The formula of the rooting medium is: improved MS+IBA 2.0 mg / L+ diethyl hexanoate 0.04 mg / L+ sucrose 15 g / L+ agar 7 g / L, pH 6.0.

[0051] The culture conditions in the rooting medium are as follows: the temperature is 25° C., the light cycle is 10 h / d, and the light intensity is 3000 Lx.

[0052] After calculation, the rooting rate is 80%.

Embodiment 3

[0054] 1) Get the current year's branches of Cyclocarya paliurus, and obtain tissue culture seedlings by in vitro culture according to conventional methods;

[0055] 2) Rooting culture: Cut out a single tissue culture seedling that is higher than 3 cm and has 2 to 3 compound leaves, and insert it into the rooting medium for 28 days; wherein:

[0056] The formula of the rooting medium is: improved MS+IBA1.5mg / L+NAA0.5mg / L+ diethyl hexanoate 0.02mg / L+ sucrose 18g / L+ agar 5.5g / L, pH6.0;

[0057] The culture conditions in the rooting medium are as follows: the temperature is 27° C., the light cycle is 10 h / d, and the light intensity is 2800 Lx.

[0058] After calculation, the rooting rate is 72%.

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Abstract

The invention discloses a cyclocarya paliurus tissue culture seedling rooting culture medium and a rooting culture method. The formula of the rooting culture medium is prepared from improved MS, 0.5 to 2.0 mg / L of IBA, 0 to 1.0 mg / L of NAA, 0.02 to 0.1 mg / L of diethyl aminoethyl hexanoate, 15 to 20 mg / L of saccharose and 5 to 8 g / L of agar, and has the pH of 5.5 to 6.0, wherein the formula of the improved MS is prepared from major elements: 700 mg / L of potassium nitrate, 600 mg / L of ammonium nitrate, 185 mg / L of magnesium sulfate heptahydrate, 60 mg / L of monopotassium phosphate and 165 mg / L of calcium chloride dihydrate, organic compounds: 100 mg / L of inositol, 1 mg / L of hydrochloric acid, 1 mg / L of pyridoxine hydrochloride, 0.2 mg / L of thiamine hydrochloride and 2 mg / L of glycine, and trace elements which are the same as an MS culture medium. The culture medium is used for performing rooting culture on cyclocarya paliurus tissue culture seedlings, and the rooting rate is greater than or equal to 70 percent.

Description

technical field [0001] The invention relates to plant tissue culture technology, in particular to a rooting medium and a rooting culture method of Cyclocarya paliurus tissue culture seedlings. Background technique [0002] Cyclocarya paliurus (Batal.) Iljinskaja is a plant of the genus Cyclocarya paliurus (Batal.) Iljinskaja in the Juglandaceae family. Known as the "giant panda of plants" and "the third tree in the medical field", Cyclocarya paliurus is a rare tree species that survived the Quaternary Ice Age. It only exists in China and is an endangered plant under national key protection. Cyclocarya paliurus is mainly distributed in Jiangxi, Zhejiang, Jiangsu, Anhui, Fujian, Taiwan, Hubei, Sichuan, Guizhou, Yunnan and other places, mostly scattered in deep mountains and old forests and some nature reserves. [0003] Cyclocarya paliurus has unique health care and medicinal value. Folks have long used the green papaya leaves to make tea, the tea has a delicate fragrance an...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001
Inventor 莫海萍苏玉卿李伯林刘云
Owner GUILIN BOLIN BIOLOGICAL TECH CO LTD
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