Preparation method of gold quantum dots and application thereof
A technology of gold quantum dots and chloroauric acid, which is applied in the field of in vivo imaging of plant cells and tissues, and the preparation of gold quantum dots, which can solve problems such as no related reports on gold quantum dots.
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Embodiment 1
[0025] Example 1 Preparation and purification of gold quantum dots
[0026] The glass containers used for experimental preparation and containing reagents were soaked in aqua regia and washed and dried. 50 mL of chloroauric acid (HAuCl 4 ) aqueous solution (10 mM, 37°C) was added to 50 mL of bovine serum albumin (BSA) aqueous solution (50 mg / L, 37°C), stirred for 5 min, and then 5 mL of sodium hydroxide (NaOH) aqueous solution (1 mol / L), react in a water bath at 37°C for 12 h. The prepared gold quantum dots were centrifuged at 14,000 r / min for 30 min, and the supernatant was added to a dialysis bag (molecular weight cut-off of 14 kDa) for dialysis for 4 days, and then sterilized by filtration with a microporous membrane with a pore size of 0.22 μm , placed at 4°C for later use.
Embodiment 2
[0027] Example 2 Characterization of gold quantum dots
[0028] A small amount of gold quantum dot solution was sonicated for 30 min and then dropped on a copper grid. After drying at room temperature, it was characterized by a JEOL JEM-2100F high-resolution transmission electron microscope (HR-TEM, Japan Electronics Co., Ltd., Japan). The particle size of gold quantum dots was calculated using Nano Measure software. The zeta potential of gold quantum dots was measured using a Zetasizer Nano ZS laser particle size analyzer (Malvern Instruments Ltd, UK). The purified and diluted gold quantum dots (400 mg / L) were irradiated with 365 nm ultraviolet light to observe its fluorescence color, and its fluorescence color at 480 nm excitation wavelength was measured with a Cary Eclipse fluorescence spectrophotometer (Varian, USA). Fluorescence spectrum, using Thermo NanoDrop 1000 spectrophotometer (Nanodrop, USA) to detect its UV-Vis absorption spectrum.
[0029] The result is as figur...
Embodiment 3
[0030] Embodiment 3 Stability analysis of gold quantum dots
[0031] 1. pH stability analysis
[0032] The purified and diluted gold quantum dots were adjusted to pH 4, 5, 6, 7, 8, 9, and 10 aqueous solutions with 1M HCl and 1M NaOH respectively, and their fluorescence at an excitation wavelength of 480 nm was measured by a fluorescence spectrophotometer strength.
[0033] The result is as figure 2 , the fluorescence intensity of gold quantum dots is the highest when the pH is 8, and the fluorescence intensity of gold quantum dots gradually weakens with the increase or decrease of pH. When the pH>7, the fluorescence intensity first increased and then decreased with the increase of time, and when the pH<7, the fluorescence intensity first fluctuated and then stabilized with the increase of time.
[0034] Two, H 2 o 2 Stability Analysis
[0035] Adding an appropriate amount of H to gold quantum dots 2 o 2 , so that H 2 o 2 The final concentrations were 1, 10, 100 and ...
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