Bacterial strain medium and preparation method thereof

A technology of bacteria culture medium and matrix particles, which is applied in the direction of fungi, etc., can solve the problems of poor water retention of PDA medium, affecting the air permeability of the pores in the bacteria bag, and easy shrinkage and deformation of the medium, so as to reduce investment in fixed assets and facilitate Production planning, widening the effect of raw material sources and application range

Inactive Publication Date: 2016-12-07
宫晶晶
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] 1. Not conducive to packaging, easy to break;
[0009] 2. Not resistant to preservation, the bacteria are easy to age;
[0010] 3. It is not conducive to transportation and mailing, and the medium is easy to shrink and deform in case of vibration or temperature change;
[0011] 4. The survival rate of the inoculation is low. Because the PDA medium has poor water retention, single matrix composition, and soft and brittle structure, the survival rate is low and the germination performance is weak when it leaves the laboratory and is mailed to the first production line for inoculation;
[0012] 5. There are few raw materials in each tube of culture medium, and the number of mycelia is limited, which is not practical, economical, and costly, which is not conducive to large-scale production needs
[0021] (3) The bacteria are added to the pores of the bacteria bag, which affects the air permeability of the pores of the bacteria bag
[0024] The branches and mesopores are not standardized, often the branches are short, the bacteria fall into the bottom of the hole, and the mycelium grows reversely from bottom to top, with a long cycle

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] The formula of embodiment 1 medium

[0068] Matrix particles 78%

[0069] Oats 6%

[0070] Corn 6%

[0071] Gypsum 0.6%

[0072] Quicklime 0.6%

[0073] Soy straw 7.3%

[0074] Sugar 1.5%,

[0075] The water content of the medium is controlled at 55%, and the pH value of the medium is 5.5.

[0076] The matrix particles are cut wood into cube particles with a particle size of 0.3-1.2 cm, and the wood is coniferous wood.

[0077] The preparation method of bacterial culture medium is:

[0078] (1) Preparation of matrix particles: cut wood into cube particles by machine;

[0079] (2) Preparation of medium;

[0080] (3) Soaking matrix particles: Soak oats and corn for beating, dissolve them in water together with sugar, mix well and soak the matrix particles until there is no dry core;

[0081] (4) Mixing material: dry mix the crushed bean straw, quicklime, and gypsum evenly, add the water after soaking the granules, mix well, add to the soaked matrix granules, mix w...

Embodiment 2

[0085] The formula of embodiment 2 medium

[0086] Matrix particles 90%

[0087] Oats 3%

[0088] Corn 3%

[0089] Gypsum 0.3%

[0090] Quicklime 0.3%

[0091] Soy straw 3%

[0092] Sugar 0.4%,

[0093] The water content of the medium is controlled at 63%, and the pH value of the medium is 7.0.

[0094] The matrix particles are cut wood into cube particles with a particle size of 0.5-1.8 cm, and the wood is hardwood.

[0095] The preparation method of bacterial culture medium is:

[0096] (1) Preparation of matrix particles: cut wood into cube particles by machine;

[0097] (2) Preparation of medium;

[0098] (3) Soaking matrix particles: Soak oats and corn for beating, dissolve them in water together with sugar, mix well and soak the matrix particles until there is no dry core;

[0099] (4) Mixing material: dry mix the crushed bean straw, quicklime, and gypsum evenly, add the water after soaking the granules, mix well, add to the soaked matrix granules, mix well, an...

Embodiment 3

[0102] The formula of embodiment 3 medium

[0103] Matrix particles 85%

[0104] Oats 4%

[0105] Corn 4.2%

[0106] Gypsum 0.6%

[0107] Quicklime 0.6%

[0108] Bean Straw 5%

[0109] Sugar 0.6%,

[0110] The water content of the medium is controlled at 60%, and the pH value of the medium is 6.

[0111] The matrix particles are cut wood into cube particles with a particle size of 0.5-1.8 cm, and the wood is hardwood.

[0112] The preparation method of bacterial culture medium is:

[0113] (1) Preparation of matrix particles: cut wood into cube particles by machine;

[0114] (2) Preparation of medium;

[0115] (3) Soaking matrix particles: Soak oats and corn for beating, dissolve them in water together with sugar, mix well and soak the matrix particles until there is no dry core;

[0116] (4) Mixing material: dry mix the crushed bean straw, quicklime, and gypsum evenly, add the water after soaking the granules, mix well, add to the soaked matrix granules, mix well, a...

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PUM

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Abstract

The invention discloses a bacterial strain medium and a preparation method thereof. The bacterial strain medium solves the problems of soft and loose states of the traditional bacterial strain medium, improves base hardness and is convenient for transport and storage. Because of matrix change, physical vibration and temperature difference adaptability are improved. The traditional medium tissue structure is changed so that matrix permeability is improved and mycelia growth oxygen demands are greatly satisfied. The matrix components are changed so that a bacterial strain quality guarantee period is prolonged. The bacterial strain medium changes nutrition components of the traditional medium, has comprehensive and reasonable nutrition, improves medium base number and effectively inhibits infectious microbes. Through use of needlebush particles and sawdust, a raw material source and an application scope are widened. Inoculation efficiency is improved and a bacterial strain cost is about 1/5 that of the traditional PDA medium.

Description

technical field [0001] The invention relates to the technical field of edible fungus breeding, in particular to a strain culture medium and a preparation method. Background technique [0002] The edible and medicinal fungi industry is the fastest growing industry in the agricultural field since my country's reform and opening up, and the production of fungi is the top priority of the edible fungus industry. The quality of the culture medium and the texture of the raw materials are crucial to the quality, vigor and ability of the strain to adapt to large-scale production. [0003] At present, the backwardness of the industry's strain production methods seriously restricts the development of the industry. It has caused thousands of households, large units, and small enterprises to produce strains regardless of their own level conditions. The production is disordered, out of control, huge economic losses, difficult for the government to supervise, and the industry It is diffic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
CPCC12N1/14
Inventor 宫晶晶
Owner 宫晶晶
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