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Method for testing antibacterial performance of probiotics antibacterial home textile

A test method and probiotic technology, applied in the directions of microorganism-based methods, microbial determination/inspection, biochemical equipment and methods, etc., can solve the problems of pollutants and germs carried by products, hidden dangers to consumers' health, etc., and reduce bacterial colonies. effect of numbers

Inactive Publication Date: 2016-12-07
VIOLET HOME TEXTILE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Because the raw materials, production, transportation and sales of traditional household products are subject to a certain degree of environmental and artificial pollution, resulting in products carrying pollutants and germs; bringing health risks to consumers; traditional household products are in the ordinary Production is carried out in an environment, the temperature and humidity in nature, harmful substances in the air, dust, and the raw materials of household products themselves carry viruses, bacteria, chemical harmful substances, etc.; a few products are even carriers of viruses and germs

Method used

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  • Method for testing antibacterial performance of probiotics antibacterial home textile

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specific Embodiment 1

[0028] A method for testing the antibacterial properties of probiotics antibacterial household textiles, comprising the steps of:

[0029] (1) Culture and preparation of Staphylococcus aureus (ATCC 6538) and Escherichia coli (8099)

[0030] Take an inoculation loop of bacteria from the slant of the 3rd to 10th generation of bacteria preservation test tube, streak on the nutrient agar plate, and cultivate at 36°C for 18 hours; use the inoculation loop to pick out the bacteria from the plate A typical bacterial colony was inoculated in 20mL of nutrient broth at a temperature of 36°C and a rotational speed of 130r / min, and shake cultured for 18 hours to prepare the inoculum suspension; reach 1*10 9 CFU / ml, this fresh bacterial solution should be used as soon as possible within 4 hours to ensure the activity of the inoculated bacteria;

[0031] Use a straw to draw 2mL from the bacterial suspension, which is the upper limit for Staphylococcus aureus and the lower limit for Escher...

specific Embodiment 2

[0050] A method for testing the antibacterial properties of probiotics antibacterial household textiles, comprising the steps of:

[0051] (1) Culture and preparation of Staphylococcus aureus (ATCC 6538) and Escherichia coli (8099)

[0052] Take an inoculation loop of bacteria from the slant of the 3rd to 10th generation of bacteria preservation test tubes, mark the bacteria on the nutrient agar plate, and cultivate them. The culture temperature is 38°C and the culture time is 24h; A typical bacterial colony was inoculated in 20mL of nutrient broth at a temperature of 38°C and a rotational speed of 130r / min, and shake cultured for 20 hours to prepare the inoculum suspension; reach 5*10 9 CFU / ml, this fresh bacterial solution should be used as soon as possible within 4 hours to ensure the activity of the inoculated bacteria;

[0053] Use a straw to draw 3mL from the bacterial suspension, which is the upper limit of Staphylococcus aureus and the lower limit of Escherichia coli...

specific Embodiment 3

[0072] A method for testing the antibacterial properties of probiotics antibacterial household textiles, comprising the steps of:

[0073] (1) Culture and preparation of Staphylococcus aureus (ATCC 6538) and Escherichia coli (8099)

[0074] Take an inoculation loop of bacteria from the slant of the 3rd to 10th generation of bacteria preservation test tube, streak on the nutrient agar plate, and cultivate it. The culture temperature is 37°C and the culture time is 21h; use the inoculation loop to pick out from the plate A typical bacterial colony was inoculated in 20mL of nutrient broth at a temperature of 37°C and a rotational speed of 130r / min, and cultured by shaking for 19 hours, and the inoculum suspension was made; the content of the bacterial solution was determined by a spectrophotometer, and the number of viable bacteria should be reach 3*10 9 CFU / ml, this fresh bacterial solution should be used as soon as possible within 4 hours to ensure the activity of the inoculat...

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Abstract

The invention discloses a method for testing the antibacterial performance of a probiotics antibacterial home textile. The method includes the following steps of culture and preparation of a staphylococcus aureus (ATCC 6538) solution and an escherichia coli (8099) solution; sample preparation; sample sterilization; sample bottling; 0-contact-time oscillation contact, dilution culture and colony count determination; result calculation. According to the method, a test sample and a control sample are put in a buffer solution containing an inoculant solution, bacteria are starved to death based on the action mechanism of a culture sample for cutting off the food chain of the bacteria, and thus the colony count is reduced; under the specified temperature and humidity conditions, the bacteriostasis rate obtained after 3 days of culture of escherichia coli and 6 days of culture of staphylococcus aureus is used for testing the antibacterial performance of the test sample.

Description

technical field [0001] The invention specifically relates to a method for testing the antibacterial properties of probiotic antibacterial household textiles. Background technique [0002] Because the raw materials, production, transportation and sales of traditional household products are subject to a certain degree of environmental and artificial pollution, resulting in products carrying pollutants and germs; bringing health risks to consumers; traditional household products are in the ordinary Production is carried out in an environment, the temperature and humidity in nature, harmful substances in the air, dust, and the raw materials of household products themselves carry viruses, bacteria, chemical harmful substances, etc.; a few products are even carriers of viruses and germs. Contents of the invention [0003] Purpose of the invention: the present invention provides a method for testing the antibacterial properties of probiotic antibacterial household textiles. [0...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/19C12R1/445
CPCC12Q1/18
Inventor 汪明星刘金抗陈永兵陈凤葛玲
Owner VIOLET HOME TEXTILE TECH
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