Single-layer biochemical dry film for detecting blood lipid and preparation method thereof
A single-layer film and dry sheet technology, applied in the field of medical devices, can solve the problems of complex dry sheet operation and low detection limit, and achieve the effects of simple operation, low detection limit and wide linear range.
Inactive Publication Date: 2016-12-07
CHANGCHUN UNIV OF SCI & TECH
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Problems solved by technology
[0008] The purpose of the present invention is to provide a single-layer film biochemical dry sheet for detecting blood lipids and a preparation method, which solves the problem of complicated operation of the prepared dry sheet, and has a wide linear range, good repeatability and stability, and can detect The characteristics of low limit, fast detection speed and simple operation
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Embodiment 1
[0032] The biochemical dry slices were prepared using single-layer chromatography filter paper with a diameter of 6 mm, a thickness of 0.3 mm, a flow rate of 130 mm / 30 min, and a water loading capacity of 10 μL. By immobilizing liquid biochemical reagents for blood lipid detection on chromatographic filter paper, a preparation method for blood lipid detection single-layer biochemical dry film was established, and the standard color scale of blood lipid detection single-layer film biochemical dry film was prepared, and the full-spectrum dry biochemical The analyzer establishes the quantitative detection standard curve of the relative intensity of reflected light of the dry film (Y) and the concentration of the blood lipid standard (X), and determines the relevant parameters of the determination, such as incubation time, sample volume, etc. So far, a single-layer film dry biochemical assay method for blood lipid detection has been established.
[0033] Let’s take cholesterol as ...
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Abstract
A single-layer biochemical dry film for detecting blood lipid and a preparation method thereof belong to the technical field of medical instruments and are characterized in that cholesterol detection reagent A and detection reagent B are immobilized to single-layer chromatographic filter paper that is attached to a cover glass; the preparation method includes: (1), cutting the chromatographic filter paper into a circle 6 mm in diameter for use; (2), spraying 9-11 MuL of the cholesterol detection reagent B to the chromatographic filter paper on the cover glass, freezing at very low temperature, lyophilizing in a dryer, and repeating 3-5 times; (3), moving to the cover glass to obtain the cholesterol detection single-layer biochemical dry film; (4), detecting on a machine; (5), preparing a detection standard curve and standard level for the dry film. The dry film and the preparation method have the advantages that linear range is wide, repeatability and stability are good, the detection limit is low, detection speed is high, operation is simple, and it is possible for a patient to make detection by himself or herself.
Description
technical field [0001] The invention belongs to the technical field of medical devices and relates to an improvement of a clinical in vitro detection reagent. Background technique [0002] Lipids contained in plasma are collectively referred to as blood lipids, mainly including cholesterol (or total cholesterol) (TC), triglyceride (TG), high-density lipoprotein (HDL) and low-density lipoprotein (LDL). Although the plasma lipid content only accounts for a very small part of the total body lipid, both exogenous and endogenous lipid substances need to enter the blood to move among various tissues. Therefore, blood lipid content can reflect the situation of lipid metabolism in the body. [0003] Epidemiological and clinical studies have confirmed that the increase in plasma cholesterol and triglyceride levels is related to the occurrence of atherosclerosis, and the incidence of atherosclerosis and coronary heart disease is negatively correlated with serum high-density lipoprote...
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Login to View More IPC IPC(8): G01N33/92
CPCG01N33/92
Inventor 于源华张洋王晓刚张司晨
Owner CHANGCHUN UNIV OF SCI & TECH