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Method for extracting general flavones from peony seed husks

A technology of peony seed shell and extraction method, which is applied in the direction of medical formula, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of low extraction efficiency and large amount of flavonoids, and achieve important economic value and ecological significance , strong applicability, suitable for large-scale production

Inactive Publication Date: 2016-12-14
铜陵亚通牡丹产业发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The patent application number 201410638164.7 discloses a method for extracting flavonoids from peony seed skins. The flavonoids in the peony seed skins are extracted by water extraction. The extraction method uses water as a solvent, and the equipment requirements are not high, the cost is low, and the environment and human non-toxic, etc., but the extraction efficiency of flavonoids is low, and the extract contains a large amount of impurities

Method used

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  • Method for extracting general flavones from peony seed husks
  • Method for extracting general flavones from peony seed husks
  • Method for extracting general flavones from peony seed husks

Examples

Experimental program
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Embodiment 1

[0030] A method for extracting total flavonoids in peony seed shells, comprising the following steps:

[0031] (1) Pulverizing the raw material peony seed shells and passing through a 60-mesh sieve to obtain peony seed shell powder;

[0032] (2) Accurately weigh 5.00g of peony seed shell powder, add ethanol solution according to the material-to-liquid ratio of 1:10 (g / mL), mix well, keep it in a 30°C water bath for 30 minutes, and homogenate for 3 minutes , the number of times of homogenization is 2, and the mass fraction of ethanol solution is 50%;

[0033] (3) Centrifuge at 4000r / min for 20min, and take the supernatant;

[0034] (4) The supernatant described in step (3), partly measures its absorbance value as a sample solution, calculates the solution concentration, thereby measures the content of flavonoids; part is concentrated under reduced pressure and freeze-dried to obtain peony seed shell Total flavonoid extract.

Embodiment 2

[0036] A method for extracting total flavonoids in peony seed shells is the same as in Example 1 except that the solid-liquid ratio in step (2) is 1:8 (g / mL).

Embodiment 3

[0038] A method for extracting total flavonoids in peony seed shells is the same as in Example 1 except that the solid-liquid ratio in step (2) is 1:12 (g / mL).

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Abstract

The invention provides a method for extracting general flavones from peony seed husks. The method comprises the following steps of (1) smashing and sieving the raw material peony seed husks to obtain peony seed husk powder; (2) accurately weighing the peony seed husk powder, adding an ethanol solution with the mass concentration of 40 percent to 95 percent according to a ratio of material to solution: 1:8 to 1:25 (g / mL), after uniformly mixing, keeping warm, standing and extracting in a water bath kettle for 0 to 90min, and homogenating for 1 to 4 times; (3) centrifuging to get a supernatant; (4) adopting part of supernatant in the step (3) as a sample solution, measuring a light absorption value of the solution, and calculating the concentration of the solution, thus measuring the content of a flavone compound; decompressing, concentrating, freezing and drying the other part of supernatant to obtain general flavone extracts in the peony seed husks. The method for extracting the general flavones from the peony seed husks, provided by the invention, is high in extraction ratio of the general flavones in the peony seed husks, and has the advantages of simplicity in operation, high applicability, suitability for mass production, and low cost.

Description

technical field [0001] The invention belongs to the field of food biotechnology, and in particular relates to a method for extracting total flavonoids in peony seed shells. Background technique [0002] Peony is a plant of the genus Paeoniae and Paeoniae, and is known as "the beauty of the country and the fragrance of heaven". Peony seeds are rich in fatty acids, proteins, 18 kinds of amino acids, various trace elements and multivitamins. Peony seed oil developed from peony seeds is a natural pollution-free food integrating nutrition and health care. Since peony seed oil was approved as a new resource food on March 22, 2011, the utilization of peony resources has become a research focus. [0003] Peony seeds are nuts protected by double layers of shell and seed shell. Peony seed oil can be extracted by removing the shells. The unsaturated fatty acid content of peony seed oil is more than 90%. It is known as the most suitable oil for human nutrition so far. Peony Seed shell...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/71A61K36/65
CPCA61K36/65A61K36/71A61K2236/333A61K2236/39A61K2236/51A61K2236/53
Inventor 李莉孙后田杨有煌
Owner 铜陵亚通牡丹产业发展有限公司
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