Method for extracting general flavones from peony seed husks
A technology of peony seed shell and extraction method, which is applied in the direction of medical formula, medical preparations containing active ingredients, plant raw materials, etc., can solve the problems of low extraction efficiency and large amount of flavonoids, and achieve important economic value and ecological significance , strong applicability, suitable for large-scale production
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Embodiment 1
[0030] A method for extracting total flavonoids in peony seed shells, comprising the following steps:
[0031] (1) Pulverizing the raw material peony seed shells and passing through a 60-mesh sieve to obtain peony seed shell powder;
[0032] (2) Accurately weigh 5.00g of peony seed shell powder, add ethanol solution according to the material-to-liquid ratio of 1:10 (g / mL), mix well, keep it in a 30°C water bath for 30 minutes, and homogenate for 3 minutes , the number of times of homogenization is 2, and the mass fraction of ethanol solution is 50%;
[0033] (3) Centrifuge at 4000r / min for 20min, and take the supernatant;
[0034] (4) The supernatant described in step (3), partly measures its absorbance value as a sample solution, calculates the solution concentration, thereby measures the content of flavonoids; part is concentrated under reduced pressure and freeze-dried to obtain peony seed shell Total flavonoid extract.
Embodiment 2
[0036] A method for extracting total flavonoids in peony seed shells is the same as in Example 1 except that the solid-liquid ratio in step (2) is 1:8 (g / mL).
Embodiment 3
[0038] A method for extracting total flavonoids in peony seed shells is the same as in Example 1 except that the solid-liquid ratio in step (2) is 1:12 (g / mL).
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