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Process for producing a particulate composition comprising an hydrous crystalline 2-o-alpha-d-glucosyl-l-ascorbic acid

A technology based on ascorbic acid and glucose, which is applied in the field of manufacturing powders containing 2-O-α-D-glucosyl-L-ascorbic acid anhydrous crystals, and can solve the problems of poor versatility of the manufacturing method and the like

Active Publication Date: 2016-12-14
HAYASHIBARA BIOCHEMICAL LAB INC
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, in the above-mentioned production method, in order to increase the production rate of ascorbic acid 2-glucoside in the reaction solution obtained by the enzyme reaction to 35% by mass or more, it is necessary to use a limited specific CGTase alone or with isoamylase and other starch debranching enzymes are used in combination, so it is not suitable as a production method with poor versatility

Method used

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  • Process for producing a particulate composition comprising an hydrous crystalline 2-o-alpha-d-glucosyl-l-ascorbic acid
  • Process for producing a particulate composition comprising an hydrous crystalline 2-o-alpha-d-glucosyl-l-ascorbic acid
  • Process for producing a particulate composition comprising an hydrous crystalline 2-o-alpha-d-glucosyl-l-ascorbic acid

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Experimental program
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Embodiment approach

[0092] 1. Definition of terms

[0093] In this specification, the following terms have the following meanings.

[0094]

[0095] The "degree of crystallinity of ascorbic acid 2-glucoside anhydrous crystals" as used in this specification means a numerical value defined by the following formula [1].

[0096] Formula 1]:

[0097] 【Number 1】

[0098]

[0099] h 100 : Analytical value of the degree of crystallinity obtained from powder X-ray diffraction characteristics of a powder standard sample containing ascorbic acid 2-glucoside anhydrous crystals substantially composed of ascorbic acid 2-glucoside anhydrous crystals

[0100] h 0 : Analytical value of the degree of crystallinity obtained from the powder X-ray diffraction characteristics of a powder standard sample containing ascorbic acid 2-glucoside substantially composed of an amorphous part

[0101] h s : The analytical value of the degree of crystallinity obtained from the powder X-ray diffraction characteristics...

Embodiment 1

[0416]

[0417] 4 mass parts of liquefied potato starches were heated and dissolved by adding 20 mass parts of water, and 3 mass parts of L-ascorbic acid were added to adjust the pH to 5.5 to obtain a substrate solution. To this, 100 units of CGT derived from Geobacillus stearothermophilus Tc-91 strain (Accession No. FERMBP-11273 of the Patent Organism Depository Center of the National Institute of Advanced Industrial Science and Technology) was added per 1 g of solid content of liquefied potato starch Enzyme crude enzyme solution (manufactured by Hayashibara Biochemical Laboratory Co., Ltd.), reacted at 55°C for 40 hours, together with ascorbic acid 2-glucoside, produced 2-O-α-maltosyl-L-ascorbic acid, 2-O-α α-glycosyl-L-ascorbic acid such as maltotriosyl-L-ascorbic acid and 2-O-α-maltotetraosyl-L-ascorbic acid.

[0418] After heating the reaction solution to inactivate the enzyme, it was adjusted to pH 4.5, and 50 units of glucoamylase agent (sold by Amano Enzyme Co., Ltd....

Embodiment 2

[0424]

[0425] When making CGTase act on a solution containing liquefied starch and L-ascorbic acid, pullulanase (marketed by Hayashibara Biochemical Research Institute of Co., Ltd., product code (EN201), pneumonia g The source of Klebsiella pneumoniae (Aerobacter aerogenes), and when crystallizing ascorbic acid 2-glucoside anhydrous crystallization, it is suitable to cool the temperature of the solution from 40°C to 35°C over 1.5 days Thereafter, except for the quasi-controlled cooling method of cooling from 35° C. to 15° C. in 0.5 days, a powder containing ascorbic acid 2-glucoside anhydrous crystals was produced in the same manner as in Example 1. In addition, the reaction liquid after glucoamylase treatment The production rate of ascorbic acid 2-glucoside in is about 29.5%. The ascorbic acid 2-glucoside content of the solution used for the crystallization of ascorbic acid 2-glucoside anhydrous crystals is 91.8% in terms of anhydrous.

[0426] This product is an anhydrou...

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Abstract

The invention provides a process for enabling the production of a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside that does not significantly cake even when the production yield of ascorbic acid 2-glucoside does not reach 35% by weight. The process for producing a particulate composition containing anhydrous crystalline ascorbic acid 2-glucoside, which comprises allowing a CGTase to act on a solution containing either liquefied starch or dextrin and L-ascorbic acid and then allowing a glucoamylase to act on the resulting solution to obtain a solution with an ascorbic acid 2-glucoside production yield of at least 27%, purifying the obtained solution to increase the ascorbic acid 2-glucoside content to a level of over 86% by weight, precipitating anhydrous crystalline ascorbic acid 2-glucoside by a controlled cooling method or pseudo-controlled cooling method, collecting the precipitated anhydrous crystalline ascorbic acid 2-glucoside, and ageing and drying the collected anhydrous crystalline ascorbic acid 2-glucoside.

Description

[0001] This application is a divisional application of the invention patent application 201280010888.6 filed on March 7, 2012. 【Technical field】 [0002] The present invention relates to a method for producing a powder containing 2-O-α-D-glucosyl-L-ascorbic acid anhydrous crystals, and more specifically, to a powder containing 2-O-α-D - A method for producing a powder of glucosyl-L-ascorbic acid anhydrous crystals. 【Background technique】 [0003] L-ascorbic acid has been used in various applications including foods, cosmetics, etc. due to its excellent physiological activity and antioxidative effect. On the other hand, L-ascorbic acid is unstable due to direct reduction, is easily oxidized and decomposed, and has major disadvantages of easily losing physiological activity. In order to solve this disadvantage of L-ascorbic acid, the present applicant, in Patent Document 1, as one of the co-applicants, disclosed 2-O-α in which one molecule of D-glucose is bonded to the hydrox...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/04C07H1/06C12P19/60C12P19/18A23L33/15
CPCC07H17/04A61K31/7048C07H1/06C12P19/18C12P19/60C07B2200/13C12Y204/01019A61K9/16A61P39/06A61P43/00C12P19/44C12P17/04
Inventor 涩谷孝伊泽精祐西本友之福田惠温三宅俊雄
Owner HAYASHIBARA BIOCHEMICAL LAB INC
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