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Composite microbial preparation for treating domestic sewage and preparation method thereof

A technology for compound microorganisms and domestic sewage, which is applied in the field of compound microorganism preparation for treating domestic sewage and its preparation, can solve the problems of complex process and high input cost of sewage treatment

Active Publication Date: 2016-12-14
深圳科鹏境美环保科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems of high input cost and complicated process in sewage treatment in the prior art, the present invention provides a compound microbial preparation for domestic sewage treatment and a preparation method thereof

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1: a kind of preparation method of compound microbial preparation for processing domestic sewage, comprises the steps:

[0039] The first step, the activation of bacteria

[0040] (1) Activation of bacteria on solid medium

[0041] Saccharomyces cerevisiae ACCC20251, Lactobacillus acidophilus ACCC11073, Bacillus subtilis ACCC10619, Pseudomonas nitroreducens ACCC04184, Acinetobacter lwoffii ACCC01 The preserved strain of Candida tropicalis (Candida tropicalis) ACCC20004 was inoculated on the slant of solid medium with an inoculation needle, and cultured at 30°C for 24-36h.

[0042] The medium of the strains is: Saccharomyces cerevisiae ACCC20251: 12Brix. Wort 1.0L, agar 15g, natural pH; Lactobacillus acidophilus ACCC11073: casein 10.0g, beef extract 10.0g , yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween80 1.0g, K 2 HPO 4 2.0g, 7H 2 O·MgSO 4 0.02g, H 2 O·MnSO 4 0.05g, CaCO 3 20.0g, 15g of agar, 1L of distilled ...

Embodiment 2

[0064] Embodiment 2: a kind of preparation method of processing domestic sewage compound microbial preparation, comprises the steps:

[0065] The first step, the activation of bacteria

[0066] (1) Activation of bacteria on solid medium

[0067] Saccharomyces cerevisiae ACCC20251, Lactobacillus acidophilus ACCC11073, Bacillus subtilis ACCC10619, Pseudomonas nitroreducens ACCC04184, Acinetobacter lwoffii ACCC01 The preserved strain of Candida tropicalis (Candida tropicalis) ACCC20004 was inoculated on the slant of solid medium with an inoculation needle, and cultured at 30°C for 24-36h.

[0068] The culture medium of the strain is. Saccharomyces cerevisiae ACCC20251: 12Brix. Wort 1.0L, agar 15g, natural pH; Lactobacillus acidophilus ACCC11073: casein peptone 10.0g, beef extract 10.0g, yeast extract 5.0g, glucose 5.0 g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween80 1.0g, K 2 HPO 4 2.0g, 7H 2 O·MgSO 4 0.02g, H 2 O·MnSO 4 0.05g, CaCO 320.0g, 15g of agar, 1L of ...

Embodiment 3

[0090] Embodiment 3: a kind of preparation method of compound microbial preparation for processing domestic sewage, comprises the steps:

[0091] The first step, the activation of bacteria

[0092] (1) Activation of bacteria on solid medium

[0093] Saccharomyces cerevisiae ACCC20251, Lactobacillus acidophilus ACCC11073, Bacillus subtilis ACCC10619, Pseudomonas nitroreducens ACCC04184, Acinetobacter lwoffii ACCC01 The preserved strain of Candida tropicalis (Candida tropicalis) ACCC20004 was inoculated on the slant of solid medium with an inoculation needle, and cultured at 30°C for 24-36h.

[0094] The medium of the strains is: Saccharomyces cerevisiae ACCC20251: 12Brix. Wort 1.0L, agar 15g, natural pH; Lactobacillus acidophilus ACCC11073: casein 10.0g, beef extract 10.0g , yeast extract 5.0g, glucose 5.0g, sodium acetate 5.0g, diammonium citrate 2.0g, Tween80 1.0g, K 2 HPO 4 2.0g, 7H 2 O·MgSO 4 0.02g, H 2 O·MnSO 4 0.05g, CaCO 3 20.0g, 15g of agar, 1L of distilled ...

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Abstract

The invention belongs to the field of microbial preparations, and in particular relates to a composite microbial preparation for treating domestic sewage and a preparation method thereof. The composite microbial preparation for treating the domestic sewage comprises rhodopseudomonas palustris ACCC10649, saccharomyces cerevisiae ACCC20251, lactobacillus acidophilus ACCC11073, bacillus subtilis ACCC10619, pseudomonas nitroreducens ACCC04184, acinetobacter lwoffii ACCC01091 and candida tropicalis ACCC20004. According to the composite microbial preparation for treating the domestic sewage and the preparation method thereof, which are provided by the invention, organic matters in the sewage are converted into simple inorganic matters through decomposition of microorganisms, so that the sewage is treated effectively.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and in particular relates to a composite microbial preparation for treating domestic sewage and a preparation method thereof. Background technique [0002] With the continuous improvement of the degree of human socialization and the increasing frequency of social and economic activities, the problem of environmental pollution is becoming more and more serious, especially the pollution of water bodies. The discharge of industrial wastewater and domestic sewage is the main factor causing water pollution. Domestic sewage contains a large amount of inorganic and organic matter. Inorganic substances such as chloride, sulfate, phosphate and sodium, potassium, calcium, iron and other carbonates, organic substances such as cellulose, starch, fat, protein and urea. Discharge into the environment promotes the growth and mass reproduction of phytoplankton, forming red tides and algal blooms. [0003...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/18C12N1/16C02F3/34C12R1/01C12R1/865C12R1/23C12R1/125C12R1/38C12R1/74
CPCC02F3/34C12N1/16C12N1/18C12N1/20
Inventor 唐清池闫青林戴斌郭银保
Owner 深圳科鹏境美环保科技有限公司
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