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A breeding method, tetraploid technology, applied in horticultural methods, botanical equipment and methods, plant gene improvement, etc., can solve the problems of high frequency of chimeric tetraploid, difficult to guarantee germplasm, long breeding cycle, etc., to achieve Increase the output of medicinal materials, shorten the time, improve the effect of quality and output
Inactive Publication Date: 2016-12-21
安徽斛生记生物科技有限公司
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[0004] Patent 200710061225.8 reports the cultivation method of Dendrobium officinale tetraploid. This method has a high mutagenesis rate but has certain defects: the breeding cycle is long, and it takes more than five years from breeding to industrial application; chimerism is four times higher in tetraploid plants. The frequency of the individual is high, and the germplasm is difficult to guarantee; the number of plants obtained is limited, and industrial production is limited
[0005] Patent 201210244240.7 reports a method for mass breeding of homologous homozygous tetraploid Dendrobium officinale. Although this method has a high yield, it has certain defects: protocorms need to be subcultured for many times, and after tetraploid plants are selected, they still need to be subcultured. 3 times, to induce differentiation and regenerate tetraploid plants, the operation is cumbersome; the induction time is as long as 72 hours and other problems
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Embodiment 1
[0023] (1) The plants of variety A and variety B are planted and cultivated according to conventional methods;
[0024] (2) Aseptically sow the seeds of different varieties of original plants in solid medium to obtain diploid protocorms of varieties A and B. The culture is static culture on MS solid medium at a temperature of 22 ℃, light time 10 hours, light intensity 1500lux, color temperature 4000K;
[0025] (3) Transfer the obtained diploid protocorms into a mixture containing 200mg / L colchicine, 0.5mg / L NAA (naphthalene acetic acid), 1mg / L 6-BA (6-benzylaminopurine), and the pH value is 5.6± 0.2 in 1 / 2MS liquid medium for mutagenesis at 30°C for 12 hours;
[0026] (4) Rinse the above-mentioned treated explants with sterile water and transfer them to MS solid medium. The light intensity is 1500lux, the color temperature is 4000K, the photoperiod is 16 hours / day, and the culture temperature is 22°C. Continue to cultivate under the conditions until the seedlings are differe...
Embodiment 2
[0031] (1) The plants of variety A and variety B are planted and cultivated according to conventional methods;
[0032] (2) Aseptically sow the seeds of different varieties of original plants in solid medium to obtain diploid protocorms of varieties A and B. The culture is static culture on MS solid medium at a temperature of 25 ℃, light time 12 hours, light intensity 3000lux, color temperature 5000K;
[0033] (3) Transfer the obtained diploid protocorms into a mixture containing 150mg / L colchicine, 1mg / L NAA (naphthalene acetic acid), 2mg / L 6-BA (6-benzylaminopurine), and the pH value is 5.6± 0.2 in 1 / 2MS liquid medium for mutagenesis at 30°C for 12 hours;
[0034] (4) Rinse the above-mentioned treated explants with sterile water and transfer them to MS solid medium. The light intensity is 3000lux, the color temperature is 5000K, the photoperiod is 16 hours / day, and the culture temperature is 25°C. Continue to cultivate under the conditions until the seedlings are different...
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Abstract
The invention provides a method for culturing tetraploid dendrobium nobile. The method comprises the following steps: selecting Dendrobium huoshanense varieties with different producing areas as plant parents; performing aseptic seeding on the plant seeds, thereby obtaining diploid protocorms; inducing the diploid protocorms by use of colchicine; transplanting the induced explant to an MS solid medium, and performing culture for seedling formation and differentiation; and detecting chromosome ploidy, selecting tetraploid-dyed seedlings and culturing the selected seedlings. The dendrobium nobile protocorms are adopted as raw materials, and tetraploid mutagenesis is performed on the dendrobium nobile protocorms without influence from seasonal climate, so that thousands of tetraploid dendrobium nobile can be obtained in one time. The method is simple, effective and practical, and can be used for shortening the traditional tetraploid breeding time, and ensuring that the roots, stems, leaves, plant heights and the like of the tetraploid dendrobium officinale are obviously larger or higher than those of original diploid dendrobium officinale, the yield of dendrobium officinale medicial materials is greatly improved, and the quality and yield of dendrobium officinale are improved; on the other hand, the method provided by the invention is used for polyploid seed selection and is an important method for breeding polyploids into new species with genetic improvement.
Description
technical field [0001] The invention relates to the technical field of plant polyploid cultivation, in particular to a method for cultivating tetraploid dendrobium. Background technique [0002] Dendrobium is a kind of herbaceous Orchidaceae plant. It is a traditional Chinese medicinal material. It has the functions of nourishing yin and nourishing stomach, clearing away heat and producing essence, enhancing immunity and inhibiting cancer. In recent years, with the development of society, people's living standards have gradually improved, and people have paid more and more attention to the quality of life. However, due to the deterioration of the environment and excessive excavation, the existing diploid Dendrobium grows slowly, has a low survival rate, and is difficult to guarantee the quality. However, due to the high investment in artificial planting and the low enthusiasm of farmers for planting, the resources of Dendrobium are on the verge of exhaustion. The domestic ma...
Claims
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