Fusobacterium necrophorum antigen, preparation method thereof, and vaccine prepared by adopting fusobacterium necrophorum antigen

A fusobacterium necroptosis antigen technology, applied in the field of fusobacterium necroptosis antigen and its preparation method and prepared vaccine, can solve the problem of vaccine containing endotoxin, achieve stable immune performance, stable and reliable immune effect

Pending Publication Date: 2016-12-21
JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The first object of the present invention is to provide a preparation method of Fusobacterium necroticum antigen, which uses trypsin to lyse the Fusobacterium necroticum thallus, then separates, and the obtained supernatant is inactivated, and the supernatant no longer contains Endotoxin, which solves the defect that the fusobacterium necroptosis inactivated whole bacteria vaccine contains endotoxin in the prior art

Method used

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  • Fusobacterium necrophorum antigen, preparation method thereof, and vaccine prepared by adopting fusobacterium necrophorum antigen
  • Fusobacterium necrophorum antigen, preparation method thereof, and vaccine prepared by adopting fusobacterium necrophorum antigen
  • Fusobacterium necrophorum antigen, preparation method thereof, and vaccine prepared by adopting fusobacterium necrophorum antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] A Fusobacterium necroticum antigen prepared by:

[0042] Bovine-derived Fusobacterium necroptosis was inoculated in anaerobic medium thioglycollate medium, the inoculum amount of Fusobacterium necroptosis was 5%, the culture temperature was 39°C, and the culture time was 24 hours, and the necrosis in the logarithmic growth phase was obtained by culture. Fusobacterium;

[0043] Centrifuge the cultured Fusobacterium necroticus bacterial liquid, and use a continuous flow centrifuge for centrifugation. When using a continuous flow centrifuge to centrifuge the culture of Fusobacterium necroticus, the centrifuge rotor and infusion tube need to be sterilized by boiling or autoclaving. Install the rotor and infusion tube;

[0044] The flow rate of the bacterial liquid into the continuous flow centrifuge is about 100mL / min, the centrifugation speed is 12000rpm, and the centrifugation is 5min to obtain bacterial sediment;

[0045] The bacterial cell precipitate is resuspended w...

Embodiment 2

[0052] A Fusobacterium necroticum antigen prepared by:

[0053] Bovine-derived Fusobacterium necroptosis was inoculated in the anaerobic medium brain-heart extract broth medium, the inoculum amount of Fusobacterium necroptosis was 8%, the temperature of cultivation was 37°C, and the cultivation time was 12h, and the culture was obtained in the logarithmic growth phase. Fusobacterium necroptosis;

[0054] Centrifuge the cultured Fusobacterium necroticus bacterial liquid, and use a continuous flow centrifuge for centrifugation. When using a continuous flow centrifuge to centrifuge the culture of Fusobacterium necroticus, the centrifuge rotor and infusion tube need to be sterilized by boiling or autoclaving. Install the rotor and infusion tube;

[0055] The flow rate of the bacterial liquid into the continuous flow centrifuge is about 110mL / min, the centrifugation speed is 12000rpm, and the centrifugation is 5min to obtain bacterial sediment;

[0056] The bacterial cell precipi...

Embodiment 3

[0063] A Fusobacterium necroticum antigen prepared by:

[0064] Bovine-derived Fusobacterium necroptosis was inoculated in the anaerobic medium brain-heart extract broth medium, the inoculation amount of Fusobacterium necroptosis was 10%, the temperature of cultivation was 35°C, and the cultivation time was 8 hours, and the culture was obtained in the logarithmic growth phase. Fusobacterium necroptosis;

[0065] Centrifuge the cultured Fusobacterium necroticus bacterial liquid, and use a continuous flow centrifuge for centrifugation. When using a continuous flow centrifuge to centrifuge the culture of Fusobacterium necroticus, the centrifuge rotor and infusion tube need to be sterilized by boiling or autoclaving. Install the rotor and infusion tube;

[0066] The flow rate of the bacterial liquid into the continuous flow centrifuge is about 120mL / min, the centrifugation speed is 13000rpm, and the centrifugation is 3min to obtain bacterial sediment;

[0067] The bacterial cell...

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Abstract

The invention relates to the field of fusobacterium necrophorum vaccines, and in particular relates to a fusobacterium necrophorum antigen, a preparation method of the fusobacterium necrophorum antigen, and a vaccine prepared by adopting the fusobacterium necrophorum antigen. The preparation method of the fusobacterium necrophorum antigen comprises the following steps: carrying out enzymolysis on fusobacterium necrophorum by adopting pancreatin, carrying out centrifugation to obtain a supernate, and carrying out inactivation treatment on the supernate to obtain the fusobacterium necrophorum antigen. Through multiple times of experiments, the method of lysing the thallus by adopting pancreatin is adopted, the obtained lysed product is separated, so that endotoxin and other invalid ingredients are removed, and the effective antigenic components are reserved; the vaccine prepared by adopting the fusobacterium necrophorum antigen obtained through the inactivation treatment overcomes the defects that after the inoculation of the existing vaccine, hardening easily forms at the injected part, and the side reactions including local swelling and pain, heating and the like occur; and the prepared vaccine is stable and reliable in immune performance.

Description

technical field [0001] The invention relates to the field of fusobacterium necroptosis vaccines, in particular to a fusobacterium necroptosis antigen, a preparation method thereof and a prepared vaccine. Background technique [0002] Foot rot is a common subacute highly contagious disease of cloven-hoofed animals such as cattle, sheep, and deer. Hoof cutin separation, and even death. Foot rot causes huge economic losses to the cattle, sheep, and deer breeding industries in my country and the world every year. [0003] At present, there is no commercial vaccine available for the clinical prevention and treatment of foot rot, mainly clinical treatment and antibiotic treatment, but the poor control effect has caused antibiotics to be abused in the animal breeding industry; The study successfully developed the inactivated Fusobacterium necroptosis whole bacteria vaccine suitable for cattle and deer. Although the immune protection rate of the vaccine is over 90%, and the immuni...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/114A61K39/39A61P31/04
CPCA61K39/114A61K39/39A61K2039/521A61K2039/552A61K2039/55505A61K2039/55511A61K2039/55566
Inventor 陈立志冯二凯程世鹏刘晓颖王克坚程悦宁佟盼盼
Owner JL TEYAN BIOLOGICAL TECH LIMITED LIABILITY
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