Microfluidic chip for Raman detection of amphetamin chloride in hair, and use method thereof

A microfluidic chip and methamphetamine technology, which is applied in Raman scattering, chemical instruments and methods, and laboratory containers, can solve the problems of short detection time limit and easy false positives, and achieve reliable and easy-to-preserve detection results , easy to carry and use

Inactive Publication Date: 2016-12-21
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The traditional methamphetamine detection method is urine analysis, but it has the disadvantages of short detection time limit and prone to false positives. In recent years, the development of hair drug testing has become an effective means of testing addicts. It can dig out the degree of drug use of drug addicts. , so the detection of methamphetamine in the hair has been highly valued by toxicology analysts

Method used

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  • Microfluidic chip for Raman detection of amphetamin chloride in hair, and use method thereof
  • Microfluidic chip for Raman detection of amphetamin chloride in hair, and use method thereof
  • Microfluidic chip for Raman detection of amphetamin chloride in hair, and use method thereof

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Embodiment 1

[0040] see figure 1 , figure 2 , the device structure of the present invention is as follows:

[0041]The main body is in the shape of a rectangular parallelepiped (length X width: 31.5X15.75mm), and is divided into upper and lower layers, including a substrate A and a cover B, and sealed alignment bonding can be achieved between the two layers.

[0042] The substrate A includes: a labeling solution pool 1 for containing immunogold sol, a washing solution pool 2 for containing buffer solution, a sample reaction and Raman detection area 4, and a waste solution pool 5 for containing stop solution , for the detection liquid pool 6 for containing the immunomagnetic antibody, for the detection liquid pool 7 for the liquid to be tested for hair extraction, wherein:

[0043] The waste liquid pool 5 is located at the rightmost end of the chip, and is connected to the detection area 4 on the left side through the fourth channel 4-1. A magnet is placed on the back of the detection ar...

Embodiment 2

[0050] The preparation process of the nanoparticles labeled with immunogold is as follows;

[0051] Take 49.5ml ultrapure water + 0.5ml HAuCl 3 Stir and disperse evenly, heat and condense under reflux, add 0.5ml Na at one time 3 Cit (trisodium citrate), the color first turned black, purple-black, and finally purple-red, and then condensed and refluxed for 15 minutes to obtain Au sol with a particle size of about 35 nm; take 1 ml of Au sol + 2.5 μl of 1mM MBA, incubate for 1.5 hours, and centrifuge to disperse ; Add 2 μl of 5 μg / ml methamphetamine antigen and incubate for 3.5 h, centrifuge to disperse, and resuspend in 1 ml of phosphate buffer; add 10 μl of 10% BSA to incubate for 1 h, and centrifuge to disperse 1 ml of phosphate buffer.

[0052] The preparation process of the immunomagnetic nanoparticle solution is as follows:

[0053] Take 0.68g FeCl 3 +0.2g ​​Na 3 Cit+20ml EG (ethylene glycol) was dissolved by ultrasonic, and 1.2g CH was added 3 COONa was stirred and mi...

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Abstract

The invention discloses a microfluidic chip for Raman detection of amphetamin chloride in hair. The microfluidic chip comprises a substrate A and a cover sheet B, and is characterized in that the substrate A comprises a labeling liquid pool (1) for holding an immunogold sol, a washing liquid pool (2) for holding a buffer liquid, a detection zone (4) for a sample reaction and Raman, a waste liquid pool (5) for holding a terminating liquid, a first detection liquid pool (6) for holding an immunomagnetic antibody and a second detection liquid pool (7) for holding a hair extraction liquid to be detected, the waste liquid pool (5) is connected to the detection zone (4) through a fourth channel (4-1), a magnet is placed at the back surface of the detection zone (4), the washing liquid pool (2) is connected to the detection zone (4) through a third channel (2-1), the third channel (2-1) is arranged between the detection zone (4) and the washing liquid pool (2), and the third channel (2-1) is provided with a valve. According to the present invention, the low concentration detection can be achieved, and the obtained result is consistent with the low content of the amphetamin chloride in the actual human hair.

Description

technical field [0001] The field involved in the present invention is microfluidic chip and its application field. In particular, it relates to a microfluidic device for pretreatment of methamphetamine in hair, rapid Raman detection of methamphetamine, and a method for using the same. Background technique [0002] The addiction and prevalence of drugs, such as methamphetamine (MAMP, commonly known as "ice"), has become an increasingly serious problem in today's world. Chronic overdose of methamphetamine can lead to confusion, hallucinations, apathy, and, in severe cases, neurobiological changes, damage to cognitive function, and possibly psychiatric disorders. Existing technologies for the detection of methamphetamine residues mainly include liquid chromatography, gas-mass spectrometry chromatography, thin-layer chromatography, etc. Although these methods are accurate in detection, they require expensive large-scale instruments, are time-consuming, and have cumbersome pretr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01L3/00G01N21/65G01N33/543
CPCB01L3/5027B01L2200/10G01N21/658G01N33/54326
Inventor 许清良周勇亮陈宏田中群
Owner XIAMEN UNIV
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