Composite inoculant for deep oil contaminated soil bioremediation process

Abstract

The invention relates to a composite inoculant for a deep oil contaminated soil bioremediation process. The composite inoculants comprises, by weight, 15-25 parts of an aspergillus niger ATCC 16404 suspension, 20-28 parts of an aspergillus versicolor CICC2474 suspension, 15-25 parts of a penicillium chrysogenum ATCC10106 suspension, 22-27 parts of a trichoderma viride ACCC30169 suspension, 25-35 parts of an aureobasidium pullulans DSM14940 suspension, and 8-15 parts of a bacillus acidophaeus ATCC 9372 suspension; and the live bacteria count of the six bacteria suspensions reaches more than 109 / mL. According to the invention, a microbial monomer degrading petroleum hydrocarbon pollutants is adopted as the starting point, comparison and screening are carried out to obtain a group of mixed bacteria composed of bacteria and fungi, and their proportion is optimized to obtain the composite inoculants. At the same time, an application method of the microbial composite inoculant and a biosurfactant for combined remediation of deep oil contaminated soil is studied. By means of the method, degradation of TPH in soil can reach 90% or more.

Description

technical field [0001] The invention belongs to the field of microorganisms, and in particular relates to a composite bacterial agent made from various microbial suspensions. Background technique [0002] Petroleum has become one of the most important energy sources for human beings. The total annual oil production has exceeded 2.2 billion tons, of which about 1.8 billion tons are produced by land oil fields. About 8 to 10 million tons of petroleum hydrocarbons enter the environment every year (excluding losses in the petroleum processing industry). In my country, nearly 600,000 tons of oil enters the environment every year, causing serious pollution of soil, groundwater, water systems and oceans. The survey report on major pollutants shows that my country produces nearly 100,000 tons of oil-contaminated soil annually, and the cumulative amount of piles is nearly 500,000 tons. After petroleum substances enter the soil, especially after entering the deep layer of the soil, ...

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to solve the problem of the current lack of deep oil-contaminated soil remediation with petroleum hydrocarbons as the main pollution source, and to provide a composite bacterial agent for microbial remediation of deep oil-contaminated soil