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Method for efficiently degrading pollutants in oil refining wastewater by using photosynthetic bacterium system

A technology for oil refining wastewater and photosynthetic bacteria is applied in the field of sewage treatment and resource utilization, which can solve the problems of complex process and strong light conditions, and achieve the effect of simple process, high-efficiency degradation, and reduction of hardware input cost and time cost.

Pending Publication Date: 2021-11-16
HEBEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the current research, only photosynthetic bacteria are used alone to treat wastewater containing glucose and other food organic matter, and strong light conditions and aeration treatment are required. The process is complicated, and it is not suitable for oil refining containing petroleum hydrocarbons and phenols. Wastewater treatment, photosynthetic bacteria have not yet been used to treat refinery wastewater and recover carotenoids, bacteriochlorophyll and coenzyme Q from it 10 Research

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  • Method for efficiently degrading pollutants in oil refining wastewater by using photosynthetic bacterium system
  • Method for efficiently degrading pollutants in oil refining wastewater by using photosynthetic bacterium system
  • Method for efficiently degrading pollutants in oil refining wastewater by using photosynthetic bacterium system

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Embodiment 1

[0031] In this embodiment, the method for efficiently degrading pollutants in oil refinery wastewater using photosynthetic bacteria strains comprises the following steps:

[0032](1) Take 5g of soil in the anoxic area of ​​the lake that can be exposed to direct sunlight, place it in a 100mL graduated cylinder, add sterilized enrichment medium to 95mL, then add 5mL of liquid paraffin, place it in an incubator, and set the temperature 30°C, light intensity 3000-4000lux, culture for 7 days, when red bacteria adhere to the soil surface, the culture medium changes from colorless to red, take 5mL of liquid and transfer to a new culture medium, enrich repeatedly until the color turns purple or deep red. The composition of the enrichment medium is: sodium acetate: 3g / L, sodium propionate: 0.3g / L, ammonium sulfate: 0.3g / L, calcium chloride: 0.1g / L, dipotassium hydrogen phosphate: 0.3g / L, Potassium dihydrogen phosphate: 0.5g / L, manganese sulfate: 0.025g / L, ferrous sulfate: 0.005g / L, ye...

Embodiment 2

[0038] In an oil refinery in Panjin City, Liaoning Province, the indicators of oil refining wastewater are: COD: 16181mg / L, NH 4 + -N: 771mg / L, NO 3 - -N: 165mg / L, NO 2 - -N: 21mg / L, chroma: 15000, conductivity: 4.14mS / cm. The refinery wastewater contains more phenols and hydrocarbons, pH6.14, neutral, and opaque. The refinery wastewater is diluted 12 times to make it transparent. After dilution, the pH is 6.8, and the pH is adjusted to 7.0. Get diluted appropriate amount of oil refinery wastewater and put it in a conical flask, and then add 30% dosage (per 1000L of diluted oil refinery wastewater, add 300L of bacteria liquid after centrifugation, the concentration of the two strains in the bacteria liquid is 102mg / L), add photosynthetic bacteria strains, light anaerobic culture at 30°C, set the light intensity gradient as: 500, 2000, 4000, 6000 and 8000lux, after a period of cultivation, carry out precipitation separation, and measure the pollutant indicators in the sup...

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Abstract

The invention relates to a method for efficiently degrading pollutants in oil refining wastewater by using a photosynthetic bacterium system. The photosynthetic bacterium system comprises Rhodopseudomonas and Pseudomonas, and the mass ratio of Rhodopseudomonas to Pseudomonas is (0.8-1.2): 1; and the photosynthetic bacterium system is added into the oil refining wastewater, and cultured under illumination and anaerobic conditions. The method specifically comprises the following steps: putting the photosynthetic bacterium system into light-permeable oil refining wastewater with the pH value close to neutral, carrying out anaerobic culture at 28-35 DEG C under the light intensity of 400-4000 lux for 3-15 days, and monitoring the pollutant index change in the wastewater and the content change of carotenoid, bacterial chlorophyll and coenzyme Q10 in the wastewater every day. According to the method, pollutants in the oil refining wastewater can be efficiently degraded, and meanwhile, high-value substances can be accumulated in cells.

Description

technical field [0001] The invention belongs to the field of sewage treatment and resource utilization, and specifically relates to a method for efficiently degrading pollutants in oil refinery wastewater by using photosynthetic bacteria strains. The method can efficiently degrade pollution in oil refinery wastewater containing more organic substances such as hydrocarbons and phenols. At the same time, it can recover high-value intracellular substances. Background technique [0002] Heavy oil occupies a large proportion of the world's oil and gas reserves. According to data, the recoverable reserves of heavy oil, super heavy oil, super heavy oil and natural bitumen are as high as 4000-6000×10 8 m 3 . Biological treatment occupies a large proportion in the wastewater treatment process. However, in activated sludge reactors, salt intrusion causes cell osmotic shock, reduces specific oxygen absorption rate, hinders cell growth rate, and interferes with enzymatic pathways, mi...

Claims

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Application Information

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IPC IPC(8): C02F3/34C12N1/20C12R1/38C12R1/01C02F103/36C02F101/16
CPCC02F3/34C12N1/20C02F2103/365C02F2101/16C02F2101/163C02F2101/166Y02W10/37
Inventor 李相昆孙钰洁
Owner HEBEI UNIV OF TECH
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