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Bacillus isothermal layer GDUTAN16 for degrading dimethyl disulfide and application of bacillus isothermal layer GDUTAN16

A technology of dimethyl disulfide and bacillus, which is applied in the field of microbial degradation, can solve the problems such as no reports of other different strains, no stratospheric bacillus that can degrade dimethyl disulfide, etc., achieve significant degradation effect

Pending Publication Date: 2022-03-15
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the research on biodegradation focuses on microbial strains that can degrade dimethyl disulfide. Studies have shown that microbial strains that can degrade dimethyl disulfide include: Bacillus cereus, spindle Lysinibacillus fusiformis (Lysinibacillus fusiformis) and Bacillus cohn (Bacillus cohn), have not seen other different bacterial strain reports for microbial strains that can degrade dimethyl disulfide; and about stratosphere Bacillus, existing Only Bacillus stratosphericus that can degrade protein or organic phosphorus is disclosed in the technology, and there are no related reports on Bacillus stratosphericus that can degrade dimethyl disulfide

Method used

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  • Bacillus isothermal layer GDUTAN16 for degrading dimethyl disulfide and application of bacillus isothermal layer GDUTAN16
  • Bacillus isothermal layer GDUTAN16 for degrading dimethyl disulfide and application of bacillus isothermal layer GDUTAN16
  • Bacillus isothermal layer GDUTAN16 for degrading dimethyl disulfide and application of bacillus isothermal layer GDUTAN16

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Embodiment 1

[0033] The isolation and screening of embodiment 1 bacterial strain

[0034] The present invention adopts the landfill leachate from Xingfeng Landfill in Guangzhou City, Guangdong Province, and screens the bacterial strain by adding dimethyl disulfide to the inorganic salt medium, and uses dimethyl disulfide as the acclimation substrate to The strains were acclimatized, and the acclimatization substrate concentrations were 1mg / L, 5mg / L, 10mg / L, 15mg / L, 20mg / L and 25mg / L.

[0035] The specific steps of domestication are as follows: first, take 10 mL of landfill leachate and add it to an inorganic salt medium containing 1 mg / L dimethyl disulfide. By analogy, gradually increase the concentration for acclimatization.

[0036] After the acclimatization, the bacterial solution with different acclimatization substrate concentrations was diluted 10 -1 ~10 -7times, and then take 0.2mL each to spread evenly in the inorganic salt medium with dimethyl disulfide as the carbon source, an...

Embodiment 2

[0037] The identification analysis of embodiment 2 bacterial strains

[0038] (1) Morphological characteristics of the strain:

[0039] Know that the bacterial strain that is screened out is Gram-positive bacterium by conventional bacterial strain physiology and biochemical identification method; The bacterial strain that screens out is placed on scanning electron microscope SEM and observes, and it is observed that its form is rod-shaped, and thalline size is (1.4 ~1.6)μm×(0.4~0.5)μm, without flagella, the result is as follows figure 1 As shown; after 24 hours of culture on nutrient agar medium, the colony shape is round, light yellow, opaque, and the diameter of the colony is 5.0-6.0 mm; the main physiological and biochemical characteristics of the strain are shown in Table 1 below.

[0040] Table 1 Physiological and biochemical characteristics of strains

[0041]

[0042]

[0043] NOTE: Reaction status is divided into positive and negative, positive shape is coded a...

Embodiment 3

[0051] Evaluation of the performance of dimethyl disulfide in the degradation environment of the bacterial strain GDUTAN16 in embodiment 3

[0052] (1) Configure inorganic salt medium:

[0053] Add 100mL of the prepared inorganic salt culture solution into the serum bottle, and place it at 121°C for high-pressure sterilization for 30min.

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Abstract

The invention discloses a bacillus isothermal layer GDUTAN16 for degrading dimethyl disulfide and application of the bacillus isothermal layer GDUTAN16. The bacillus isothermal layer GDUTAN16 is preserved in the China Center for Type Culture Collection on November 22, 2021, and the preservation number is CCTCC NO: M 20211470. The bacillus stratosphaericus GDUTAN16 strain has good degradation performance on the dimethyl disulfide in the environment, and the degradation rate of the bacillus stratosphaericus GDUTAN16 strain on the dimethyl disulfide with the concentration of 5-25 mg / L reaches 94% or above when the bacillus stratosphaericus GDUTAN16 strain is used for 72 h; the bacillus homothermal layer GDUTAN16 strain can be used as a novel microbial bacterium for biologically treating odor pollutants, is used for degrading dimethyl disulfide, provides a novel treatable efficient strain and method for treating the odor pollutants, and enriches a microbial strain resource library.

Description

technical field [0001] The invention belongs to the technical field of microbial degradation. More specifically, it relates to a stratospheric bacillus GDUTAN16 degrading dimethyl disulfide and its application. Background technique [0002] Dimethyl disulfide (DMDS), also known as dimethyl disulfide, is an important chemical product, widely used in the petroleum industry, and can be used as a raw material for pesticides to produce the insecticide "fenthion". , has contact and stomach poisoning effects on pests, has certain permeability to crops, and is also used as a preservative and anti-scorch agent, a pre-vulcanizing agent in the process of petroleum hydrodesulfurization, a regeneration agent and a plasticizer in the rubber industry; in addition, it is also used It can be used in food industry as food flavor, pesticide raw material, etc. As a common sulfide organic odor pollutant, dimethyl disulfide has a strong, long-lasting and irritating garlic odor. When the human b...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34B01D53/84B01D53/48C12R1/07C02F101/30
CPCC12N1/20B09C1/10C02F3/345B01D53/84B01D53/48C02F2101/40B01D2251/95Y02A50/20
Inventor 安太成廖文李桂英梁志梳崔元镕
Owner GUANGDONG UNIV OF TECH
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