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Paracoccus acridae B54 and application thereof

A paracoccus, b54cgmccno.17653 technology, applied in the field of microorganisms and microbial wastewater treatment, can solve the problems of human and animal health threats, increased treatment difficulty, difficult degradation, etc., and achieves wide temperature adaptability, obvious removal effect, and good degradation effect.

Active Publication Date: 2019-11-15
INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, large-scale breeding can greatly shorten the growth cycle of livestock and poultry, increase production, and reduce costs. On the other hand, due to the large-scale accumulation of pollutants, the difficulty of treatment increases, so the environmental pollution is not to be underestimated. watch for
Livestock wastewater has high concentration of suspended solids, COD cr High content, BOD 5 High organic phosphorus content, high ammonia nitrogen content, difficult to transport, and difficult to degrade. In addition, livestock and poultry wastewater contains a large number of pathogenic microorganisms, parasite eggs, and residual heavy metal elements and antibiotics. The air, water, and soil environment cause serious harm, destroy the natural ecological balance, and pose a great threat to human and animal health

Method used

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  • Paracoccus acridae B54 and application thereof
  • Paracoccus acridae B54 and application thereof
  • Paracoccus acridae B54 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The isolation and identification of embodiment 1 bacterial strain

[0018] Mix fresh pig urine collected from a pig farm in Daxing, Beijing, with tap water at a ratio of 1:4, add fresh pig manure with a mass fraction of 5% and mix evenly, after standing for 24 hours, take the upper layer of liquid as pig breeding sewage for later use .

[0019] The above sewage was randomly divided into groups and naturally fermented on a shaking table at 30°C and 200rpm for 3 days, and then samples were taken to detect changes in the content of COD, ammonia nitrogen and total phosphorus. The group with the most obvious decrease in pollutant content was selected and 5 mL of fermentation broth was inoculated into the enrichment medium. Cultivate at 30°C, 200rpm shaker for 24h, then continue to inoculate 5mL culture solution into new enrichment medium, and culture repeatedly for 5 generations.

[0020] Enrichment medium: the above-mentioned pig breeding sewage was sterilized at 121°C for...

Embodiment 2

[0031] Degradation of ammonia nitrogen sewage by bacterial strains of embodiment 2

[0032] LB medium: 5 g of yeast extract, 10 g of tryptone, 10 g of sodium chloride, and 1 L of distilled water.

[0033] Ammonia nitrogen sewage: glucose 0.62g, sodium acetate 2g, ammonium sulfate 1g, Vickers salt 50mL, distilled water 1L, pH 7.5. Vickers salt: 5g of dipotassium hydrogen phosphate, 2.5g of magnesium sulfate heptahydrate, 2.5g of sodium chloride, 0.05g of ferrous sulfate heptahydrate, 0.05g of manganese sulfate tetrahydrate, 1L of distilled water.

[0034] The above medium was sterilized at 121°C for 30 min before use.

[0035] Pick a single bacterial colony from the plate and place it in LB liquid, and cultivate it at 30°C for 24 hours to activate it. Under aseptic conditions, add the B54 cells that have been centrifuged to remove the culture medium into the ammonia nitrogen sewage at a volume fraction of 5%, and set the culture condition to 30°C. ℃, 200rpm, samples were take...

Embodiment 3

[0038] Embodiment 3 bacterial strains are to the degradation of livestock and poultry breeding sewage

[0039] The pretreatment method of livestock and poultry breeding sewage is to mix fresh pig urine collected from a pig farm in Daxing, Beijing, with tap water at a ratio of 1:4, then add fresh pig manure with a mass fraction of 5%, stir evenly, and let stand After 24 hours, the upper layer liquid was taken as laboratory livestock and poultry breeding sewage for later use.

[0040]Add 0.5% molasses to the above-mentioned livestock and poultry breeding sewage as an additional carbon source, and use it for the degradation experiment of the strain after high temperature and high pressure sterilization. Pick the B54 single colony on the plate into the LB liquid, incubate at 30°C for 24 hours to activate, and insert the B54 cells after centrifugation to remove the culture medium into the livestock and poultry sewage at a volume fraction of 5% under sterile conditions, and set The...

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Abstract

The invention discloses paracoccus acridae (paracoccus acridae) B54 for synergistically degrading COD and ammonia nitrogen of livestock and poultry breeding waste water and an application of the paracoccus acridae B54. The bacterium is preserved in the China General Microbiological Culture Collection Center on 29 April, 2019 and is abbreviated as CGMCC, the address is No. 3, No 1 yard, Beichen West Road, Chaoyang district, Beijing, and the preservation number is CGMCC No.17653. The strain has obvious degradation effects on COD, ammonia nitrogen and total phosphorus.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, in particular to the technical field of microbial wastewater treatment. Background technique [0002] In recent years, with the continuous adjustment of my country's agricultural industrial structure, the scale and intensification of the livestock and poultry breeding industry have continued to increase. On the one hand, large-scale breeding can greatly shorten the growth cycle of livestock and poultry, increase production, and reduce costs. On the other hand, due to the large-scale accumulation of pollutants, the difficulty of treatment increases, so the environmental pollution is not to be underestimated. watch for. Livestock wastewater has high concentration of suspended solids, COD cr High content, BOD 5 High organic phosphorus content, high ammonia nitrogen content, difficult to transport, and difficult to degrade. In addition, livestock and poultry wastewater contains a large numb...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F103/20
CPCC02F3/34C02F2103/20C12N1/20C12N1/205C12R2001/01
Inventor 李少杰曹先贺孙宪昀张振颖
Owner INST OF MICROBIOLOGY - CHINESE ACAD OF SCI
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