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Detection method of fungus polysaccharide

A technology of fungal polysaccharides and detection methods, which is applied in the detection of fungal polysaccharides and the field of polysaccharide detection, can solve the problem of polysaccharides having no identification function, and achieve the effects of low requirements for detection instruments, saving time, and preventing harm

Inactive Publication Date: 2016-12-21
JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can eliminate the interference of starch, but has no discrimination effect on other polysaccharides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Detection of Grifola frondosa polysaccharide content in Grifola frondosa polysaccharide extract mixed with dextrin:

[0022] A. Sample preparation

[0023] (1) Weigh 0.2g of Grifola frondosa polysaccharide extract mixed with dextrin, put it in a 100ml volumetric flask, add water and heat to 80°C, dissolve the Grifola frondosa polysaccharide extract mixed with dextrin for 30 minutes, and then set capacity;

[0024] (2) Take 5ml of the solution in step (1), put it in a centrifuge tube, add 100ml of 95% ethanol, shake well, let stand for 10min, then centrifuge at 4000r / min for 10min, discard the supernatant, repeat 3 times, get sediment;

[0025] (3) Take the sediment, add 85°C hot water to dissolve it, put it in a beaker, the ratio of sediment to hot water is 1:1.35, add 0.1ml of moss enzyme by weight ratio, and use the water bath method to bathe in water at a temperature of 40°C 1h to get the mixed solution, take out the mixed solution and heat to boiling, keep boilin...

Embodiment 2

[0031] Example 2 Detection of Ganoderma lucidum polysaccharide content in Ganoderma lucidum polysaccharide extract mixed with starch

[0032] A. Sample preparation

[0033] (1) Weigh 0.2 g of Ganoderma lucidum polysaccharide extract mixed with starch, put it in a 250ml volumetric flask, add water and heat to 80°C, and ultrasonically dissolve the Ganoderma lucidum polysaccharide extract mixed with starch for 30 minutes, and then make up to volume;

[0034] (2) Take 5ml of the solution in step (1), put it in a centrifuge tube, add 100ml of 95% ethanol, shake well and let stand for 10min, then centrifuge at 4000r / min for 10-20min, discard the supernatant, repeat 3 times, a precipitate was obtained;

[0035] (3) Take the sediment, add 85°C hot water to dissolve it, put it in a beaker, the ratio of sediment to hot water is 1:1.35, by weight, and add 0.1ml of moss enzyme or cellulase, and use the water bath method at 40 Put the mixture in a water bath for 1 hour at a temperature o...

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PUM

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Abstract

The invention discloses a detection method of fungus polysaccharide. The detection method comprises the following steps: preparing to obtain a sample; using glucose solution as a standard substance; using a sulfuric acid-anthrone or sulfuric acid-phenol method to prepare the standard curve, wherein the absorbance is ordinate and the concentration is abscissa in the standard curve; developing with the sulfuric acid-anthrone or sulfuric acid-phenol method; measuring the absorbance with a spectrophotometer to obtain the concentration of the anhydrous glucose in the corresponding sample from the standard curve according to the measured absorbance; and re-calculating to obtain the content of polysaccharide in the sample. The detection method provided by the invention is easy to operate, saves time, is low in requirement for detection instruments, can solve the detection problems encountered in quality control for various testing organizations, producers and customers, and prevents adulterated products from endangering the market.

Description

technical field [0001] The invention belongs to the field of health food or food, and relates to a detection method of polysaccharides, in particular to a detection method of fungal polysaccharides. technical background [0002] The research on carbohydrates has a history of hundreds of years, but for a long time, people's understanding of carbohydrates is only a kind of energy substance or structural substance, and there is no in-depth research on its physiological activity. With the development of life science, more and more attention has been paid to the role of carbohydrates in high-level life phenomena, and the research on carbohydrates has become a new focus of scholars. In recent years, the polysaccharide extraction industry has developed rapidly, especially in China, where the polysaccharide extraction industry represented by the extraction of natural plant polysaccharides and fungal polysaccharides is in a leading position in the world. The polysaccharide extractio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N1/28
CPCG01N21/31G01N1/28
Inventor 仲延龙张莉沈井旺唐苏阳周娟施磊
Owner JIANGSU JIANGDAYUAN ECOLOGICAL BIOLOGICAL TECH
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