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Protein co-loading chemotherapy drug and radiotherapy drug and application of protein

A chemotherapeutic drug and drug technology, applied in the field of medicine, can solve the problems of poor biocompatibility of nanocarriers, complicated material preparation process and high cost, achieve good water solubility and biocompatibility, simple preparation method, and improve hypoxia. Effect

Active Publication Date: 2017-01-04
SUZHOU INNOVATIVE BIOMATERIALS & PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, some existing nanocarriers have poor biocompatibility and will stay in the body for a long time, and the preparation process of the material is complicated and the cost is high, which limits further applications.
Synthesis of nanomaterials co-loaded with radiotherapeutics and chemotherapeutics for combination therapy and imaging of tumors using a simple method has not been reported

Method used

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  • Protein co-loading chemotherapy drug and radiotherapy drug and application of protein
  • Protein co-loading chemotherapy drug and radiotherapy drug and application of protein
  • Protein co-loading chemotherapy drug and radiotherapy drug and application of protein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Preparation of proteins co-loading chemotherapeutic and radiotherapy drugs 131 I-HSA-PTX

[0077] The preparation process is as figure 1 As shown in -a, albumin HSA is used as the substrate, and chemotherapy drugs and radionuclides are transferred.

[0078] Take 100 μCi of Na 131 I, add 1ml of 2mg / ml HSA aqueous solution, then add 30μL of 10mg / ml chloramine T solution (dissolved in PB buffer), shake and shake for 20 minutes, and use a 10kDa ultrafiltration tube to ultrafilter to remove untreated Reacted Na 131 I and chloramine T, to give 131 I labeled HSA solution. Then add 13 μL of 20 mg / ml PTX (dissolved in ethanol) (the amount of PTX added will change the size of the nanoparticles, the more the amount of PTX, the larger the particle size of the nanoparticles), stir overnight, and centrifuge at 14800 rpm for 5 Minutes to remove the precipitate, the product 131 I-HSA-PTX dispersed in the supernatant

Embodiment 2

[0079] Example 2 Preparation of proteins co-loading chemotherapeutic and radiotherapy drugs 131 I-HSA-PTX

[0080] The preparation process is as figure 1 As shown in -b, the albumin HSA is used as the substrate, and the chemotherapeutic drugs and radionuclides are transferred.

[0081] Add 13ul of 20mg / ml PTX (dissolved in ethanol) to 1ml of 2mg / ml HSA aqueous solution, stir overnight, centrifuge at 14800 rpm for 5 minutes to remove the precipitate, and the intermediate HSA-PTX is dispersed in the supernatant. Then add 100uCi of Na to the supernatant 131 The 10mg / ml chloramine T solution (dissolved in the PB damping fluid) of 1 and 30ul, shaking shakes 20 minutes, with the ultrafiltration tube ultrafiltration of 10KDa, remove unreacted Na 131 I and chloramine T to give the final product 131 I-HSA-PTX.

Embodiment 3131I

[0082] Example 3 131 Characterization of I-HSA-PTX

[0083] Made to embodiment 1 or 2 131I-HSA-PTX is characterized, embodiment 1 or 2 gained 131 The laser dynamic light scattering results of I-HSA-PTX are as follows figure 2 As shown, the hydration radius of pristine HSA and radiolabeled HSA is about 8nm, radiolabeling did not change the size of HSA; the intermediate HSA-PTX and the final product 131 The hydration radius of I-HSA-PTX is about 110nm, radiolabeling did not change the material size and loading of PTX.

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Abstract

The invention relates to the technical field of medicine, in particular to protein co-loading a chemotherapy drug and a radiotherapy drug and application of the protein. In some embodiments, I-131 and PTX are loaded to albumin. The protein co-loading the chemotherapy drug and the radiotherapy drug has the good water solubility and biocompatibility and can achieve combined therapy of chemotherapy and radiotherapy, effectively reduce pressure among tumor tissue, improve anoxia of tumors and improve homogeneous-phase distribution of materials in the tumors to improve the therapy effect of radiotherapy. In addition, a preparation method of the protein is very simple.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a protein co-loading chemotherapy drugs and radiotherapy drugs and its application. Background technique [0002] Cancer is one of several major malignant diseases that threaten human health in the 21st century. Although a large amount of human, material and financial resources have been invested in the prevention and treatment of cancer for decades since the 1950s, the progress made by human beings in this regard is still very limited. [0003] Surgery, radiotherapy and chemotherapy together are called the three major treatment methods for cancer. Chemotherapy is the abbreviation of chemical drug therapy, which achieves the purpose of treatment by using chemotherapy drugs to kill cancer cells. Chemotherapy is a means of systemic treatment. Regardless of the route of administration (oral, intravenous, and body cavity administration, etc.), chemotherapy drugs will spread throug...

Claims

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Application Information

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IPC IPC(8): A61K47/48A61K31/337A61K51/08A61P35/00
CPCA61K31/337A61K51/081
Inventor 杨凯刘庄田龙龙陈倩
Owner SUZHOU INNOVATIVE BIOMATERIALS & PHARM CO LTD
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