35 results about "Co loading" patented technology

The invention discloses an oligonucleotide-based nano carrier for co-delivering drug and gene and a preparation method of the nano carrier. The method comprises the following steps: 1, mixing an oligonucleotide solution with a drug solution, standing at room temperature for 1-100 minutes, and then mixing with siRNA to obtain a mixed solution; 2, adding a cationic polymer solution to the mixed solution obtained in the step 1), standing and incubating at room temperature for 1-100 minutes, so as to obtain a polycation compound co-loading drug and gene; 3, adding an anionic polymer solution to the polycation compound obtained in the step 2), standing and incubating at room temperature for 1-100 minutes, so as to obtain an oligonucleotide-based nano carrier for co-delivering drug and gene. The preparation process of the oligonucleotide-based nano carrier for co-delivering drug and gene is simple and feasible and the oligonucleotide-based nano carrier for co-delivering drug and gene can be applied to treatment of a plurality of cancers such as liver cancer, lung cancer, breast cancer and ovarian cancer.

The invention relates to a DCs vaccine based on phospholipid hybrid polymersome jointly encapsulating an antigen and dual immunoagonists, a preparation method and application thereof. The phospholipidhybrid polymersome which can jointly load a model antigen OVA and two types of TLR agonists (TLR7/8 and TLR4) is used for stimulation in vitro of the DCs so as to realize the effective phagocytosis of DCs cells. The rapid and long-term immunostimulatory effect on the DCs is achieved by the internal and external co-loading of the OVA antigen. The synergistic effect of the two types of TLR agonistssignificantly enhances the immune response after antigen stimulation; the phospholipid hybrid polymersome which jointly encapsulates the antigen and the dual immunoagonists can effectively promote the activation and maturation of the DCs, increases the level of cross-presentation, promotes the migration of the DC vaccine to secondary lymphoid organs, and produces a strong specific cytotoxic T lymphocytes (CTLs) killing effect, thereby effectively killing tumor cells and realizing the immunotherapy of the DCs vaccine on tumors.

The invention discloses a preparation method of a semiconductor nano particle/carbon point porous monolithic catalyst and application of nitrogen fixation to ammonia formation, which belongs to the technical field of nano catalysis, nano materials and the like. The method mainly comprises the following steps of: impregnating and adsorbing a glucose-containing nickel nitrate and cobalt nitrate solution, a ligand solution of terephthalic acid and triethylene diamine by melamine foam in sequence, performing microwave radiation to prepare a CoNi-MOF/melamine foam composite material containing glucose, oxidizing and pyrolyzing the CoNi-MOF/melamine foam composite material, so that a porous monolithic composite material, namely a semiconductor nanoparticle/carbon dot porous monolithic catalyst is prepared by the co-loading semiconductor Co3O4 and NiO nanoparticles and the carbon quantum dots on a carbon-nitrogen matrix. The preparation method has the advantages of low cost of raw materials,simple preparation process, low reaction energy consumption and industrial application prospect. The catalyst is used for electrocatalysis of nitrogen fixation into ammonia and has good electrochemical activity.

The invention discloses a synthetic method of a metal Co-loaded N-doped three-dimensional porous carbon material having excellent electrocatalytic oxygen reduction performance. The method comprises the following steps: dissolving ZIF-67 and [VEIm][BF-4] in an absolute N,N-dimethyl formamide solution, and performing uniform dispersion; adding 2,2'-azobis, performing continuous ultrasonic wave; in oil bath, contrinoulsy stirring the components and then stopping a reaction; dumping the components in an acetone solution, performing centrifugation and pumping filtration to obtain precipitates, and drying and grinding the precipitates to obtain the powder product; roasting the powder product in a tubular furnace to obtain the black powder product; and using dilute sulfuric acid for processing the black powder product to obtain the metal Co-loaded N-doped three-dimensional porous carbon material. The metal Co-loaded N-doped three-dimensional porous carbon material has the beneficial effect that the metal Co is taken as a metal source, the earth reserves of the metal Co is abundant, the cost is low, during a material preparation process, the raw material utilization rate is high, the metal Co-loaded N-doped three-dimensional porous carbon material is environment friendly, and has excellent electrocatalytic oxygen reduction performance, methanol anti-toxic effect is stable, and the method has large economic benefit and social benefit, and has wide application prospect.

The invention belongs to the field of preparation of nanometer materials and particularly relates to a TiO2-Ti3C2-CoSx nanocrystal photocatalyst and a preparation method of the TiO2-Ti3C2-CoSx nanocrystal photocatalyst. ZIF-67 is taken as a template, a high-conductivity substance is used as a charge transfer bridge, and a TiO2-Ti3C2-CoSx heterostructure with the constrained nanocrystal size is prepared in one step through the solvothermal technique. The transfer and utilization efficiencies of a photoproduction carrier pair in a TiO2 are improved through the co-loading of the ZIF-67 derived CoSx and Ti3C2, meanwhile, the problem of easy aggregation of the TiO2 at the high temperature is solved, and the defect of weaker photocatalytic hydrogen generation property is overcome. In addition, the reaction condition is mild, the costs of the materials are low, the operation is simple, the requirement on equipment is low, and the theoretical and technical support is provided for designing andpreparing the efficient green photocatalyst.

The invention provides a co-loaded adriamycin and siRNA (small interfering ribose nucleic acid) carrier capable of removing pegylation and a synthesis method thereof. Firstly, cystamine modification is carried out on terminated methoxyl polyethylene glycol, so that cystamine modified mPEG is obtained; then a carbodiimide method is utilized for bonding an RAFT (reversible additive fragment transfer) polymerization chain transfer agent onto the end of a cystamine modified mPEG chain, so that a macromolecule RAFT chain transfer agent; then RAFT polymerization is carried out, so that a terminated methoxyl polyethylene glycol-poly(N-(3-dimethylamine propyl) methacrylamide)-poly(N-t-butyloxycarboryl propylene hydrazide) triblock copolymer is obtained; finally a protective group (namely t-butyloxycarboryl) is removed, so that a terminated methoxyl polyethylene glycol-poly(N-(3-dimethylamine propyl) methacrylamide)-polypropylene hydrazide triblock copolymer is obtained. The synthesis method has the advantages that raw materials are available, reaction conditions are mild, molecular weight of a polymer and length of a chain of each block in the obtained carrier can be easily controlled, after adriamycin is loaded, nanoparticles can be self assembled, pharmaceutical effect is high, and co-loading of a chemotherapeutic drug and a gene-based drug is realized.

Described herein are flash nanoprecipitation methods capable of encapsulating hydrophobic molecules, hydrophilic molecules, bioactive protein therapeutics, or other target molecules in amphiphilic copolymer nanocarriers.

The invention discloses a liquid crystal gel nanoparticle freeze-dried powder capsule for treating a gastric ulcer and a preparation method thereof. The liquid crystal gel nanoparticle freeze-dried powder capsule is prepared from the following components in parts by weight: 3.5 to 5.5 parts of active pharmaceutical ingredient, 3.5 to 4.8 parts of drug combination, 23 to 27 parts of natural phospholipid, 37 to 45 parts of fatty glyceride, 7 to 9 parts of poloxamer, 11.4 to 14.2 parts of cosolvent and 3.3 to 5.8 parts of freeze-drying protecting agent, wherein the active pharmaceutical ingredient is at least one of pantoprazole, omeprazole, lansoprazole and bismuth potassium citrate; the drug combination comprises amoxicillin and clarithromycin. The invention also provides the preparation method of the liquid crystal gel nanoparticle freeze-dried powder capsule. The preparation method comprises the following steps of firstly preparing a liquid crystal gel nano precursor, then freeze-drying the liquid crystal gel nano precursor to make into freeze-dried powder, and finally making into the capsule. A liquid crystal gel nanoparticle of the liquid crystal gel nanoparticle freeze-dried powder capsule can be used for co-loading drugs with different polarity; the activity and synergistic action of the drugs are enhanced; the overall drug effect is improved; the slow release effect is good; the duration of the drug effect is long, and the drugs are small in size, more convenient to take and longer in storage period.

The invention discloses a novel copper sulfide based high-efficiency anti-tumor composite material and a preparation method and application thereof. The method comprises the following steps of preparing copper sulfide nanoparticles in a sodium alginate aqueous solution, co-loading the copper sulfide nanoparticles and glucose oxidase to mesoporous organic silicon, and performing quick synthesis toobtain the novel copper sulfide based high-efficiency antitumor composite material. The composite material has the advantages of being convenient to prepare, convenient to use, notable in anti-tumor effects and the like. Compared with the prior art, the composite material utilizes the thermal effects and photodynamic effects of the copper sulfide nanoparticles in a united manner; the glucose oxidase is added, the photodynamic effects of the copper sulfide nanoparticles can be notably reinforced, and high-efficiency tumor treatment based on nanometer copper sulfide can be realized. The composite material has important application value in the field of photoinduction high-efficiency tumor treatment.

The invention discloses a cationic polymer for co-loading drugs and genes and application of the cationic polymer. A constructed cationic PCL-ss-P (GHA-co-PEGMA) polymer is rich in side chains, so that the polymer has good biocompatibility and water solubility; in addition, a shell layer is rich in hydroxy groups, which can promote transmembrane transport of a drug/gene complex and improve the transcription and expression of the genes in cells. Under the condition of higher glutathione, cracking of a main chain in the drug/gene complex is caused, and the release of drugs in polymeric micellesis caused, thereby achieving the purpose of inhibiting tumor cell proliferation. The release of the drugs and the transcription and expression of the genes can be combined to treat the lung cancer, and the drug resistance of anti-cancer drugs in the cells is effectively overcome. The cationic polymer for the co-loading drugs and genes and the application of the cationic polymer have the advantagesthat the used experimental conditions are milder, the structure of the cationic polymer is easy to control, the operation is simple, the raw materials are easy to obtain, purification is easy, and the cationic polymer is suitable for industrial production; therefore, the cationic polymer can be used as a common carrier of the anti-cancer drug and cancer suppressor genes, and has a larger market application prospect in the future.

The invention discloses tumor-targeting nanoparticles co-loading chemotherapy drug and nucleic acid and a preparation method thereof; the preparation method comprises: (1) preparing polylysine-grafted Beta-cyclodextrin derivative; (2) preparing nanoparticles loading chemotherapy drug; (3) preparing nanoparticles co-loading chemotherapy drug and nucleic acid; (4) preparing tumor-targeting nanoparticles co-loading chemotherapy drug and nucleic acid. The preparation process of the invention is simple and easily operable and saves time and power, and a load material used herein is high in bio-safety and is good in bio-compatibility and biodegradability, zero in toxicity and free of immunogenicity; the tumor-targeting nanoparticles co-loading chemotherapy drug and nucleic acid have typical core-shell structure, are 150-200 nm in particle size, are effective in loading both chemotherapy drug and nucleic acid to cells that highly express CD44 molecules, can inhibit cell proliferation, and have significant in-vivo and in-vitro tumor targeting property.

The invention relates to a nano biopesticide composition and a preparation method thereof. The preparation method comprises the following steps: (1) mixing and emulsifying a first solution with a second solution to obtain a first emulsion, wherein the first solution contains an amphiphilic polymer, a hydrophobic pesticide and an organic solvent, and the second solution contains a hydrophilic pesticide and water; (2) mixing and emulsifying the first emulsion obtained in the step (1) with a third solution containing an emulsifying agent and water to obtain a second emulsion; and (3) removing an organic solvent in the second emulsion obtained in the step (2), and separating to obtain the nano biopesticide composition. According to a principle of forming an oil/water-in-water nano-micelle from the amphiphilic polymer through a double-emulsion method, the hydrophilic pesticide and the hydrophobic pesticide are entrapped in different layers of a nano-capsule respectively, thus realizing co-loading of the hydrophilic pesticide and the hydrophobic pesticide. Through a simple and easy method, a problem of combining the hydrophilic pesticide and the hydrophobic pesticide in the nano biopesticide is solved.

The invention discloses a preparation method of a nano-Fe Pd catalyst. The method comprises the steps of mixing an Fe<3+> water solution and a Pd <2+> water solution with a mesoporous material for dipping; then drying, roasting and finally reducing in the atmosphere of H2 to obtain the FePd catalyst. The invention also discloses the nano-FePd catalyst and the application of the nano-Fe Pd catalyst. According to the preparation method, the mesoporous material is used for carrying out space limitation on the growth of nano particles, so that the nano particles which are fine in particle size and uniform in distribution can be obtained, and the catalyst has higher activity; the activity of the catalyst is further improved by the co-loading of Fe and Pd; furthermore, the application method is simple, the types and the proportions of active components can be adjusted, the components have the characteristic of multi-component and multi-valence coexistence, and the activity of the catalyst can be improved by the synergistic effect of the components. The preparation method of the nano particles is simple; an oxidizing agent is environment-friendly; the nano-FePd catalyst can directly react at room temperature and has the characteristic of multiple functions and application, thus having application prospect of industrialization.

The invention relates to a ternary composite nano system based on methoxy polyethylene glycol-polylactic acid/polyethylene glycol vitamin E succinate and a derivative thereof/poloxamer . The ternary composite nano system has a diversified structure and is used for carrying out single loading or multidrug co-loading on various types of hydrophobic and hydrophilic drugs, the particle size is 50 to 100 nm, and the drug loading capacity and the encapsulation efficiency are high. The ternary composite nano system can be swallowed into cells through passive targeting of EPR (Enhanced Permeability and Retention Effect), excretion of MDR (Multidrug Resistance) protein on drugs can be avoided, and meanwhile, the polyethylene glycol vitamin E succinate and the derivative thereof have different-mechanism anti-MDR function with the poloxamer, so that the ternary composite nano system is capable of realizing mutual supplementation of multiple different anti-MDR mechanisms, and has a better anti-MDReffect.

The invention provides a tumor cell nuclear targeted drug-loading nanoparticle comprising a functional polypeptide modified heptamethine cyanine dye and a preparation method and application thereof. Amethod for directly modifying a functional polypeptide of the heptamethine anthocyanin dye is adopted, the water solubility and optical stability of the heptamethine cyanine dye are improved, and theheptamethine cyanine dye is co-loaded with an anthracene ring chemotherapy drug to obtain the drug-loading nanoparticle having a tumor cell nuclear targeting function. The direct modification of thefunctional peptide of the heptamethine anthocyanin dye is realized, the problem of poor water solubility of the heptamethine cyanine dye is effectively solved, the drug-loading nanoparticle prepared by co-loading the anthracene ring chemotherapy drug enhances the uptake of the drug-loading nanoparticle by tumor cells, the aggregation and distribution of tumor cell nuclei in a nuclear-approaching area are realized, targeting of the tumor cell nuclei is realized, and the uptake of a photosensitizer by the tumor cells is improved.

The invention provides a silica nano material delivery system for capturing and treating circulating tumor cells. The silica nano material is prepared from a magnetic mesoporous silica nano microsphere, a siRNA (small interfering Ribonucleic Acid) medicine and a targeting antibody. The circulating tumor cells (CTCs) are used as liquid biopsy specimens representing a primary tumor, and help to carry out histological identification in real-time and noninvasive manners. The silica nano material delivery system, based on loading the siRNA medicine into the magnetic mesoporous silica nano (FE3O4-MSNs (Mesoporous Silica Nanoparticles)) microsphere and through modification, can be used for recognizing and binding the targeting antibodies (modified by double antibodies CD133 and an EPCAM (Epithelial Cell Adhesion Molecule)) of surface biomarkers of the CTCs; a brand-new FE3O4-MSNs_siRNA@CD133-EPCAM co-loading system is finally developed, and the system can be used for preliminarily realizing the targeting capturing and inhibition on the CTCs.

The invention discloses a nano-carrier capable of realizing co-delivery of genes and medicines and a preparation method of the nano-carrier, and in particular relates to a nano-carrier capable of realizing co-loading and co-delivery of genes and medicines. The medicine delivery system is formed through self-assembly of a cation prodrug polymer and the genes through the electrostatic interaction between the cation prodrug polymer and the genes, wherein the cation prodrug polymer can release active medicines through the hydrolysis by esterases in the cells, after entering into the cells under the phagocytosis, the nano-composition escapes from an endosome under the effect of the buffer capacity of polyamines on a polymer, and releases the medicines ad the genes in the cytoplasm, so that the medicines and the genes can inhibit the tumor proliferation together.

The invention provides a portable and detachable pillar stability and bottom plate load transfer simulation device, and belongs to the technical field of pillar stability and bottom plate load transfer simulation devices. The device includes a top pressure loading part, side pressure loading parts, a bottom supporting part and a baffle plate. The top pressure loading part includes a top beam, a pressure head, hydraulic pumps, and an upper bearing plate and two upper pressing plates for pressing against the top of a rock simulator. The number of the side pressure loading parts is two, and the side pressure loading parts are equidistantly arranged on the two sides of the pressure head. Each side pressure loading part includes a side beam, side bearing plates, side pressure plates, side pressure applying bolts, screw rods and springs. The bottom supporting part includes a bottom beam arranged opposite to the top beam. The baffle plate is connected to the front side and the back side of each side beam through connecting pieces. The device can portably and detachably achieve servo loading and constant pressure loading of different parts of the upper part of the rock simulator and side pressure loading and upper and lateral co-loading of the rock simulator.

The invention discloses a delivery system capable of realizing co-loading a gene and a drug, a preparation method of the delivery system, and application. The invention belongs to the technical field of nano-drug preparations. The delivery system capable of realizing co-loading the gene and the drug is formed by combining the gene and positive ion pharmacosomes formed by self-assembly of an environmental response type lipophilic prodrug. The lipophilic prodrug is formed by connecting the head of a hydrophilic dendrimer to a hydrophobic antineoplastic drug or a prodrug of the hydrophobic antineoplastic drug by using an environmentally sensitive bond. The environmentally sensitive bond can break under specific environmental conditions, so that the hydrophobic antineoplastic drug or the prodrug of the hydrophobic antineoplastic drug can be separated from the hydrophilic dendrimer, and the disassembly of the positive ion pharmacosomes is caused to release the gene. The gene can be compressed by the pharmacosomes to form the drug co-delivery system while the encapsulation rate and the drug loading capacity are improved by the pharmacosomes. The effects of gene and drug synergy therapy or sequential therapy can be realized.

The invention relates to a poly-lipidosome co-loading SERS (Surface-Enhanced Raman Scattering) substrate with reactivity, and a construction method and application thereof. The biocompatibility of a poly-lipidosome material and the ion exchangeability of an ion liquid hydrophilic component are mainly used for co-loading gold nanoparticles and an organic and organic component on a poly-lipidosome substrate, and a novel SERS detection system with the biocompatibility is established. By use of the novel SERS detection system which is established by the invention and combines the ion liquid base poly-lipidosome with the gold nanoparticles, an organic and inorganic hybridization way is different from a conventional Raman system, a probe does not need to be loaded on the metal substrate, and instead, the probe and the metal are jointly loaded on the same substrate to realize SERS detection. By use of the surface ion exchangeability of the substrate, a probe molecule does not need to be subjected to sulfhydrylation preprocessing to realize the joint immobilization of the gold nanoparticles and the probe molecule, and probe molecule intrinsic Raman signal enhancement can be obtained.

The invention relates to the technical field of medicine, in particular to protein co-loading a chemotherapy drug and a radiotherapy drug and application of the protein. In some embodiments, I-131 and PTX are loaded to albumin. The protein co-loading the chemotherapy drug and the radiotherapy drug has the good water solubility and biocompatibility and can achieve combined therapy of chemotherapy and radiotherapy, effectively reduce pressure among tumor tissue, improve anoxia of tumors and improve homogeneous-phase distribution of materials in the tumors to improve the therapy effect of radiotherapy. In addition, a preparation method of the protein is very simple.