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Poly-lipidosome co-loading SERS (Surface-Enhanced Raman Scattering) substrate with reactivity, and construction method and application thereof

A construction method, a technique for polylipid, applied in nanotechnology for sensing, nanotechnology for materials and surface science, nanotechnology, etc., capable of addressing reactivity limitations, surface binding of organic or inorganic components Weak ability and other problems, to achieve the effect of good stability

Inactive Publication Date: 2019-09-06
LIAONING UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the structure of conventional liposomes is difficult to maintain stability, and its surface has relatively weak binding ability to organic or inorganic components, which limits the reactivity and fails to play a better role.

Method used

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  • Poly-lipidosome co-loading SERS (Surface-Enhanced Raman Scattering) substrate with reactivity, and construction method and application thereof
  • Poly-lipidosome co-loading SERS (Surface-Enhanced Raman Scattering) substrate with reactivity, and construction method and application thereof
  • Poly-lipidosome co-loading SERS (Surface-Enhanced Raman Scattering) substrate with reactivity, and construction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) The preparation method of polysome-gold nanoparticles (Polysome-Au) is as follows: the synthetic method is as follows: figure 1 shown.

[0049] 1. Ionic liquid-based liposome (Liposome)

[0050] Take 30.00mL toluene in a 100mL round-bottomed flask, add 2-methylimidazole (3.05mmoL, 0.25g), triethylamine (3.65mmoL, 0.51mL) and bromo-11-decene (6.10mmoL, 1.33mL), reacted at 90°C for 48h. Cool to room temperature after completion of the reaction, remove the amine salt solids by suction filtration, evaporate the solvent to dryness from the obtained filtrate, wash with n-hexane several times, and evaporate the solvent to dryness again, the resulting product is mixed with acetonitrile-ethyl acetate (volume ratio about 1:3 ) recrystallization, and vacuum drying to obtain a yellow-white powder, which is a liposome monomer.

[0051] Dissolve 100.0 mg of liposome monomer in 100.0 mL of deionized water, and disperse it ultrasonically for 1 hour to obtain a clear and transpar...

Embodiment 2

[0081] Example 2 SERS detection of Polysome-Au-MO

[0082] (1) Sample preparation:

[0083] Add excess methyl orange (MO) to a certain concentration of Polysome-Au aqueous solution, and carry out ion exchange reaction under magnetic stirring. After the reaction, the product was centrifuged and washed, and dried to obtain Polysome-Au (Polysome-Au-MO) modified by the organic anion Raman probe methyl orange (MO).

[0084] The prepared Polysome-Au-MO was smeared on a glass slide and flattened for SERS detection. The test conditions are as follows, excitation wavelength: 633nm, power: 1%, instrument: Renishaw in Via (UK). For relevant SERS characterization see Figure 18 .

[0085] As an important azo dye, methyl orange is not only widely used as an indicator, but also often used as a probe molecule in Raman detection systems due to its structural characteristics and vibration modes. Based on this, in order to detect the detection performance of the ionic liquid-based polylipo...

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Abstract

The invention relates to a poly-lipidosome co-loading SERS (Surface-Enhanced Raman Scattering) substrate with reactivity, and a construction method and application thereof. The biocompatibility of a poly-lipidosome material and the ion exchangeability of an ion liquid hydrophilic component are mainly used for co-loading gold nanoparticles and an organic and organic component on a poly-lipidosome substrate, and a novel SERS detection system with the biocompatibility is established. By use of the novel SERS detection system which is established by the invention and combines the ion liquid base poly-lipidosome with the gold nanoparticles, an organic and inorganic hybridization way is different from a conventional Raman system, a probe does not need to be loaded on the metal substrate, and instead, the probe and the metal are jointly loaded on the same substrate to realize SERS detection. By use of the surface ion exchangeability of the substrate, a probe molecule does not need to be subjected to sulfhydrylation preprocessing to realize the joint immobilization of the gold nanoparticles and the probe molecule, and probe molecule intrinsic Raman signal enhancement can be obtained.

Description

technical field [0001] The invention belongs to the field of liposome nanocomposite materials, and in particular relates to the construction and application of an ionic liquid-based polyliposome co-loaded SERS substrate. Background technique [0002] Surface-enhanced Raman scattering (SERS) has shown unique advantages in many fields such as chemistry, physics and biology, especially in The application of detection in aqueous solution of biological system is more and more important. Different from other spectroscopic techniques, SERS spectroscopic detection has higher requirements on the substrate. First, for the detection of substances in vivo, the substrate should have good biocompatibility, high sensitivity and low detection limit. In addition, in order to adapt to the complex biological environment in the body, substrates with better selectivity or solid-phase extraction function will greatly promote the improvement of Raman detection performance. [0003] The "contact...

Claims

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Application Information

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IPC IPC(8): G01N21/65B82Y30/00B82Y15/00B82Y40/00
CPCG01N21/658B82Y30/00B82Y15/00B82Y40/00
Inventor 张谦张海冉夏立新裴婷婷王慧婷
Owner LIAONING UNIVERSITY
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