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Application of cetuximab and collagen scaffold loaded with cetuximab to preparation of drug for repairing spinal cord injury

A collagen scaffold, a technology for spinal cord injury, applied in the medical field, can solve problems such as no clinical reports of spinal cord injury repair

Active Publication Date: 2017-01-04
WUQI BIOMEDICAL TECH (JIANGSU) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the EGFR antibody that has been used clinically is Cetuximab, but this drug is mainly used for the clinical treatment of tumor diseases (such as rectal cancer, esophageal cancer, etc.) [Malik H, Khan AZ, Berry DP, Cameron IC, Pope I, Sherlock D , Helmy S, Byrne B, Thompson M, Pulfer A, Davidson B. Liver resection rate following downsizing chemotherapy with cetuximab in metastatic colorectal cancer: UK retrospective observational study. Eur J Surg Oncol. 2015; 41(4): 499-505; Tian X, Zhou JG, Zeng Z, Shuai T, Yi LJ, Ma L, Wang Y, Cao H, Song GM. Cetuximab in patients with esophageal cancer: a systematic review and meta-analysis of randomized controlled trials. Med Oncol. 2015 ; 32(4):127.], but there is no clinical report for spinal cord injury repair

Method used

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  • Application of cetuximab and collagen scaffold loaded with cetuximab to preparation of drug for repairing spinal cord injury
  • Application of cetuximab and collagen scaffold loaded with cetuximab to preparation of drug for repairing spinal cord injury
  • Application of cetuximab and collagen scaffold loaded with cetuximab to preparation of drug for repairing spinal cord injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1. EGFR (epidermal growth factor receptor) Cetuximab (cetuximab) can promote the elongation of neurofilaments of cerebellar granule neuron cells in vitro under the condition of myelin protein inhibition.

[0041] With the cerebellar granule neuron cells of newborn 7-day rats (isolation and culture method: immerse the animal in 75% ethanol for 2-5min. In an ultra-clean bench, decapitate, separate the cerebellum, take it out and put it in pre-cooled PBS, Carefully remove blood vessels and meninges. Cut them into pieces with curved scissors, add DMEM high-glucose culture medium, and gently blow with a blunt-tipped thick-mouth dropper until the tissue clumps disappear. Then use high temperature Press-sterilized 400-mesh nylon membrane filter, centrifuge at 1000rpm for 5min, discard the supernatant, inoculate 20000-30000 cells per well into a 48-well plate containing 20% ​​fetal bovine serum in DMEM high-glucose medium, and culture in advance Coated with 200ng myeli...

Embodiment 2

[0051] Embodiment 2, prepare the spinal cord injury functional nerve regeneration collagen scaffold material containing EGFR (epidermal growth factor receptor) Cetuximab (cetuximab):

[0052] The ordered nerve regeneration collagen scaffold material (5 mm in length and 2 mm in diameter) prepared from bovine fascia was soaked in 30 μL of Cetuximab liquid (purchased from Mercurion, Germany) with a concentration of 5 μg / μL (containing 150 μg Cetuximab). Pharmaceutical company, the commercial specification is 100 mg / 20 ml / bottle), so that it is completely absorbed by the nerve regeneration collagen scaffold, and incubated on ice for 30 minutes to prepare the spinal cord injury functional nerve regeneration collagen scaffold material containing EGFR Cetuximab.

[0053] The corresponding form is as picture 2, picture 2(A) is the morphology of ordered nerve regeneration collagen scaffoldpicture ; picture 2(B) is the morphology of collagen scaffolds for spinal cord injury functional...

Embodiment 3

[0054] Example 3. Treatment of 5mm canine T8 total spinal cord transection injury with spinal cord injury functional nerve regeneration collagen scaffold material containing EGFR Cetuximab:

[0055] 1) Prepare a 5mm canine T8 spinal cord total transection injury model, and transplant functional collagen scaffold materials to the injured area:

[0056] Firstly, a canine spinal cord T8 total transection injury model was prepared (such as picture 3A), select 6 Beagle dogs about 1 year old, weighing about 7-9 kg, after anesthesia, locate the T8 segment of the spinal cord with magnetic resonance, open the T7-T9 lamina to expose the spinal cord tissue, and dissociate the T8 segment Transect 5mm to make canine acute T8 full transection injury model; then bridge the prepared functional nerve regeneration collagen scaffold material in the injury area. Then connect seamlessly. Intraoperative electrophysiological detection of the disappearance of somatosensory evoked potentials and act...

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Abstract

The invention discloses the application of cetuximab and a collagen scaffold loaded with cetuximab to preparation of a drug for repairing spinal cord injury, and also provides a nerve regeneration collagen scaffold material loaded with cetuximab. The preparing method comprises the steps of 1, making the nerve regeneration collagen scaffold material absorb cetuximab to obtain the nerve regeneration collagen scaffold material containing cetuximab; 2, incubating the nerve regeneration collagen scaffold material loaded with cetuximab to obtain the drug. Tests prove that both cetuximab and the collagen scaffold loaded with cetuximab can repair spinal cord injury, promote nerve regeneration and / or reduce spinal cord injury part gliocyte proliferation, and have great practical guiding significance in application to clinical spinal cord injury repair in future.

Description

technical field [0001] The invention belongs to the medical field, and in particular relates to the application of cetuximab and a collagen scaffold loaded with the substance in the preparation of medicines for repairing spinal cord injuries. Background technique [0002] The spinal cord is an important part of the central nervous system. Its main function is to transmit nerve information between the brain and the periphery, and it is also the low-level center of many simple reflex activities. Spinal cord injury (SCI) is more common in bruises, falls, traffic accidents, sports injuries and some natural disasters such as earthquakes and mine accidents. Spinal cord injury usually manifests as a loss of sensory and voluntary motor functions below the injured segment, and unlike peripheral nerves, it is difficult for spinal cord neurons to regenerate spontaneously into or across the injured area after injury [Neumann S, Bradke F, Tessier-Lavigne M, Basbaum AI. Regeneration of s...

Claims

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Application Information

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IPC IPC(8): A61L27/54A61L27/24
Inventor 戴建武肖志峰陈冰赵燕南李星
Owner WUQI BIOMEDICAL TECH (JIANGSU) CO LTD
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