Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Spleen mesenchymal stem cell with immune adjustment function, preparation method of cell and application of cell

A mesenchymal stem cell and spleen technology, applied in the field of spleen mesenchymal stem cells and their preparation, can solve problems affecting scientific research and application, removal, etc., and achieve the effect of inhibiting T lymphocyte proliferation, high purity, and simple operation

Pending Publication Date: 2017-01-04
CHINESE PEOPLES LIBERATION ARMY AIR FORCE GENERAL HOSPITAL
View PDF4 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when this strategy is applied to the culture of Sp-MSCs, it encounters difficulties. The reason is that the spleen is rich in a large number of hematopoietic cells such as macrophages, which have a strong ability to attach to culture bottles and are difficult to pass passage. culture removal
Hematopoietic cells doped in Sp-MSCs often cause immune responses, seriously affecting related scientific research and applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Spleen mesenchymal stem cell with immune adjustment function, preparation method of cell and application of cell
  • Spleen mesenchymal stem cell with immune adjustment function, preparation method of cell and application of cell
  • Spleen mesenchymal stem cell with immune adjustment function, preparation method of cell and application of cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Embodiment 1, a kind of method for separating and cultivating Sp-MSCs

[0068] 1. Isolation and culture of Sp-MSCs

[0069] 1. Separation of the spleen

[0070] Healthy female C57 mice aged 6-8 weeks were killed by neck dislocation. After soaking in 75% alcohol for 3-5 minutes, the spleen was isolated under aseptic conditions. The surface tissue was removed with scissors and tweezers, and the complete spleen was removed with tweezers.

[0071] 2. Removal of hematopoietic cells in the spleen

[0072] Fully grind it in a culture dish with a syringe handle for 5 minutes in an ultra-clean bench, rinse it with PBS 3-5 times, remove most of the hematopoietic cells in the spleen, and obtain the spleen stromal tissue ( figure 1 A).

[0073] 3. Collagenase digestion of spleen matrix tissue and primary culture

[0074] Place the spleen matrix tissue isolated in step 2 into collagenase solution for digestion (put 3-5 mouse spleen matrix tissues in one milliliter of collagenase...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a spleen mesenchymal stem cell with an immune adjustment function, a preparation method of the cell and an application of the cell. By the aid of the strategy of removing hematopoietic cell-digestion matrix tissues to obtain Sp-MSCs-passage purification, MSCs are cultured in an isolated manner from the spleen, and a large number of Sp-MSCs with high cell purify are obtained by isolation. Further, the Sp-MSCs have high immune adjustment function, heterogenetic antigen and homologous antigen activated T lymphocyte proliferation and activation can be inhibited, and inflammatory cytokine secretion of T lymphocyte is inhibited.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a spleen mesenchymal stem cell with immune regulation function and its preparation method and application. Background technique [0002] Mesenchymal stem cells (MSCs) are stem cells with multilineage differentiation potential and high self-renewal ability. MSCs were first discovered in bone marrow by Friedenstein et al., and then researchers successively isolated them from bone, umbilical cord, fat and other tissues. Under suitable conditions, MSCs can be expanded in large quantities and differentiated into cells of various tissue types such as osteoblasts, adipocytes, endothelial cells, and nerve cells. They are currently one of the most commonly used seed cells in the field of tissue engineering. In addition, MSC also has the ability to support hematopoiesis, can regulate the differentiation of hematopoietic stem cells into hematopoietic cells of various lineages, and is an import...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0775C12N5/0783
CPCC12N5/0668C12N5/0636C12N2509/00
Inventor 王恒湘丁丽郭子宽朱恒
Owner CHINESE PEOPLES LIBERATION ARMY AIR FORCE GENERAL HOSPITAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com