Enriching and analyzing method of circulating tumor cells of colorectal cancer
A technology for tumor cells and colorectal cancer, applied in the field of medical detection and analysis, can solve the problems of inability to conduct in-depth analysis of CTC, high cost of instruments and consumables, and limitation of large-scale application, so as to improve the detection rate of CTC, low cost and low cost Effect
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[0054] Example 1
[0055] A method for enrichment and analysis of circulating tumor cells in colorectal cancer, including the following steps:
[0056] A. Collection of peripheral blood mononuclear cells
[0057] 1. Use EDTA anticoagulation tube to collect about 10mL of patient blood sample.
[0058] 2. Using a pipette, measure 8 mL of blood sample into a BD Vacutainer CPT tube with the same mark.
[0059] 3. Centrifuge at 1700g room temperature for 25 minutes.
[0060] 4. After centrifugation, the white layer under the plasma layer is monocytes and platelets. Carefully aspirate about half of the plasma, and transfer the remaining plasma and cell layer (about 2 mL) to a sterile 15 mL centrifuge tube.
[0061] 5. Add RPMI 1640 medium to the above centrifuge tube to make the total volume 10 mL. Tighten the cap of the centrifuge tube and invert the centrifuge tube 5 times to mix the cells.
[0062] 6. Centrifuge at 300g for 15 minutes and remove the supernatant.
[0063] 7. Add 1mL PBS to the...
Example Embodiment
[0129] Example 2
[0130] A method for enrichment and analysis of circulating tumor cells in colorectal cancer, including the following steps:
[0131] A. Collection of peripheral blood mononuclear cells
[0132] 1. Use EDTA anticoagulation tube to collect about 10mL of patient blood sample.
[0133] 2. Using a pipette, measure 8 mL of blood sample into a BD Vacutainer CPT tube with the same mark.
[0134] 3. Centrifuge at 1700g room temperature for 25 minutes.
[0135] 4. After centrifugation, the white layer under the plasma layer is monocytes and platelets. Carefully aspirate about half of the plasma, and transfer the remaining plasma and cell layer (about 3 mL) to a sterile 15 mL centrifuge tube.
[0136] 5. Add RPMI 1640 medium to the above centrifuge tube to make the total volume 10 mL. Tighten the cap of the centrifuge tube and invert the centrifuge tube 5 times to mix the cells.
[0137] 6. Centrifuge at 300g for 15 minutes and remove the supernatant.
[0138] 7. Add 1mL PBS to the...
Example Embodiment
[0204] Example 3
[0205] A method for enrichment and analysis of circulating tumor cells in colorectal cancer, including the following steps:
[0206] A. Collection of peripheral blood mononuclear cells
[0207] 1. Use EDTA anticoagulation tube to collect about 10mL of patient blood sample.
[0208] 2. Using a pipette, measure 8 mL of blood sample into the BD Vacutainer CPT tube with the same mark.
[0209] 3. Centrifuge at 1700g room temperature for 25 minutes.
[0210] 4. After centrifugation, the white layer under the plasma layer is monocytes and platelets. Carefully aspirate about half of the plasma, and transfer the remaining plasma and cell layer (about 2 mL) to a sterile 15 mL centrifuge tube.
[0211] 5. Add RPMI 1640 medium to the above centrifuge tube to make the total volume 10 mL. Tighten the cap of the centrifuge tube and invert the centrifuge tube 5 times to mix the cells.
[0212] 6. Centrifuge at 300g for 15 minutes and remove the supernatant.
[0213] 7. Add 1mL PBS to t...
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