Preparation method for improving yield of epidemic cerebrospinal meningitis A group capsular polysaccharide

Abstract

The invention discloses a preparation method for improving the yield of epidemic cerebrospinal meningitis A group capsular polysaccharide. The method comprises the following steps of (1) starting strain and passage: starting the epidemic cerebrospinal meningitis A group strain passage onto a hemin epidemic cerebrospinal meningitis half-integral culture medium; putting the materials into a CO2 (carbon dioxide) cell culture box to perform culture by 8-percent CO2 at 36.5 DEG C; using a half-integral solid culture medium for culturing the second generation; using an epidemic cerebrospinal meningitis half-integral liquid culture medium for culturing the third generation in a constant-temperature oscillation oscillating table; (2) inoculating the materials into a seed tank to be cultured: inoculating the epidemic cerebrospinal meningitis A group seeds obtained through culture into the seed tank; performing constant temperature stirring and ventilation culture; (3) performing fermentation in a fermentation tank: after the epidemic cerebrospinal meningitis A group fifth-generation seeds obtained through culture are inoculated into the seed tank, maintaining the constant temperature of 36 DEG C; performing deep layer ventilation and 220rpm stirring culture; culturing the epidemic cerebrospinal meningitis A group bacteria to the later stage of the logarithmic phase or the early period of the still period (at the moment, the bacterium concentration reaches 10000 to 20000 million/mL); after the formaldehyde sterilization, removing the thalli, and collecting supernate; (4) obtaining polysaccharide after purification. The process provided by the invention has the advantage that the yield of the epidemic cerebrospinal meningitis A group capsular polysaccharide can be effectively improved.

Description

technical field [0001] The invention belongs to the field of preparation of meningococcal meningitis group A capsular polysaccharide, in particular to a preparation method for improving the yield of meningococcal meningitis group A capsular polysaccharide. Background technique [0002] Neisseria meningitidis (N. meningitidis), referred to as meningococcus (meningococcus), is the pathogenic bacteria of epidemic cerebrospinal meningitis (referred to as meningitis). For more than 100 years, ECM has been prevalent or sporadic all over the world, and it is one of the main acute respiratory infectious diseases of human beings. After long-term epidemiological monitoring in many countries, it was found that the disease has the characteristics of periodic epidemics. Italy found three epidemics in its country during 1915-1934. There have also been five national pandemics in my country. In 1938, 1949, 1959, 1967 and 1977, epidemic peaks appeared about every 8-10 years. According to ...

AI Technical Summary

Problems solved by technology

[0004] To prepare the meningococcal group A conjugate vaccine, the first step is to obtain the meningococcal meningitis group A capsular polysaccharide. The current traditional production method produce...