Whole range C-reactive protein test kit and detection method thereof

A reactive protein and kit technology, applied in biological tests, measuring devices, material inspection products, etc., can solve the problems of timely diagnosis of unwell emergency and clinical patients, long time for C-reactive protein detection, and complicated operation, and achieves reduction of non-specific The effect of heterosexual adsorption, high affinity and high sensitivity

Pending Publication Date: 2017-01-04
TAIZHOU ZECEN BIOTECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0009] The technical problem to be solved by the present invention is to overcome the problems in the prior art that the detection time of C-reactive protein is long, the operation is complicated, the repeatability is poor, and it is not suitable for the needs of emergency and clinical patients for timely diagnosis, and to provide a high-accuracy Kit for determination of BGP content in human serum by magnetic particle chemiluminescence

Method used

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  • Whole range C-reactive protein test kit and detection method thereof
  • Whole range C-reactive protein test kit and detection method thereof

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Experimental program
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Embodiment

[0056] 1. Operating procedures for preparing magnetic bead buffer: Take the preparation of 1L as an example:

[0057] 1. According to the preparation amount, select a suitable container and add 0.7kg / 700ml of pure water;

[0058] 2. Weigh 6.02g of Tris and 0.99g of NaN3 in a container, mix well, and stir at room temperature for 2 hours;

[0059] 3. Check the pH of the solution, it should be around 10;

[0060] 4. Adjust the pH value to 8.0;

[0061] 5. Measure 2.76ml of Tween-20; weigh 0.99g of neomycin sulfate; 0.03g of tetracycline; 4.97g of BSA in a container, stir for 1 hour, and adjust the pH value to 8.0±0.05;

[0062] 6. Set the volume to 1L, adjust the pH value to 8.0±0.05;

[0063] 7. Filter with a 0.22um filter and store at 4°C.

[0064] 2. Preparation process of magnetic particle reagent

[0065] Activate ferric oxide microspheres with a diameter of 0.1 micron with glutaraldehyde, mix at room temperature for 4 hours, wash with 0.01mol / L PBS pH7.4 buffer solutio...

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Abstract

The invention discloses a whole ranged C-reactive protein test kit. The test kit comprises a main reagent bag, a standard curve card and a quality control serum quality-control list; the main reagent bag comprises a calibrator, a CRP antibody reagent A, a CRP antibody reagent B, a magnetic particle reagent and a quality control serum. A reaction system close to a homogeneous phase is provided by combining a chemiluminescence technique and immune magnetic particles, by adopting a one-step reaction mode, the detection sensitivity and precision are greatly improved, the detection range is expanded, the reaction time is greatly shortened, the time from sample adding to test result obtaining is less than 35 min, and the time is obviously shorter than similar kits; multiple samples can be determined simultaneously on a full-automatic chemiluminescence instrument, high-throughput and rapid determination of C-reactive protein is achieved, the accuracy and the specificity are high, and the precision and the detection efficiency are greatly improved.

Description

technical field [0001] The invention belongs to the technical field of immune detection and analysis, and relates to a magnetic particle chemiluminescent immunoassay kit for detecting the content of C-reactive protein (CRP) in serum and a detection method thereof. Background technique [0002] C-reactive protein (C-reactiveprotein, CRP) is synthesized by hepatocytes, produced during fetal period, and transmitted by non-maternal placenta. Its production mechanism is: when the body is infected or the tissue is damaged, macrophages and other white blood cells are activated to produce interleukin-6 (IL-6), interleukin-1 (IL-1), tumor necrosis factor Cytokines such as TNF-a and other mediators, these cytokines and mediators reach the liver and stimulate hepatocytes and epithelial cells to synthesize CRP. Structurally, CRP contains 5 polypeptide chain subunits, which are non-covalently combined into a disc-shaped polymer with a molecular weight of 115,000 to 140,000. CRP is a typ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/543
CPCG01N33/6854G01N33/54326G01N2333/4737G01N2800/32G01N2800/7095
Inventor 丁建华刘振国李圆沈怡陈秋霞王鼎军
Owner TAIZHOU ZECEN BIOTECH CO LTD
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