Method for determining water-soluble vitamins with semi-automatic sample treatment HPLC (high performance liquid chromatography) technology

Abstract

The invention discloses a method for simultaneously determining multiple water-soluble vitamins in serum with a semi-automatic sample treatment HPLC (high performance liquid chromatography) technology. The HPLC method is adopted, and chromatographic conditions are as follows: a mobile phase A is a monopotassium phosphate solution, a mobile phase B is methanol, gradient elution is adopted, and the multiple water-soluble vitamins are selected from the vitamin B1, the vitamin B2, the vitamin B6, the vitamin B9, the vitamin B12, the vitamin C, nicotinic acid or nicotinamide. The pretreatment of the serum comprises steps as follows: methanol or acetonitrile is firstly adopted to precipitate protein, a supernatant is taken after centrifugation and extracted by a semi-automatic solid-phase extraction instrument, an eluent adopts an aqueous methanol solution, and a collected post-column solution is subjected to nitrogen blow-drying until reaching the appropriate volume. According to the method, the multiple water-soluble vitamins in the serum are detected with the HPLC method, qualitative and quantitative detection can be realized, the specificity and the sensitivity are high, the flux is high, the operation is simple, the cost is low, a result is objective and easy to analyze, and the method is particularly suitable for clinical popularization and application.

Description

technical field [0001] The invention relates to a method for simultaneously measuring multiple water-soluble vitamins in serum by using a semi-automatic sample processing liquid chromatography technology, and belongs to the technical field of vitamin detection. Background technique [0002] Vitamins are a class of low-molecular compounds necessary for the body to maintain normal metabolism and functions. They are one of the seven major nutritional elements of the human body. They are divided into fat-soluble and water-soluble vitamins. Vitamins in the human body must be kept in an appropriate range to be healthy for the human body. Beneficial, whether it is lacking or increasing, it will bring harm to the human body. Vitamins B1, B2, B6, B9, B12, vitamin C, niacin, and niacinamide are all water-soluble vitamins. When the human body lacks these vitamins, it will show different symptoms: B1 deficiency can easily cause beriberi, etc., B2 deficiency can cause fat B6 deficiency ...

AI Technical Summary

Problems solved by technology

However, due to the lack of scientific guidance, the current status of the use of vitamin products is very chaotic. In order to improve various functions of the body, some people spend a lot of money on health care products and blindly supplement vitamins. According to the survey of the Chinese Nutrition Society in 2014, Chinese residents Diseases caused by excess or unbalanced nutrition are increasing year by year

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