Koelreuteria paniculata breeding method

Abstract

The invention discloses a koelreuteria paniculata breeding method and relates to the technical field of koelreuteria paniculata seedling production. The method includes a) a step of implant inoculating and culturing, namely a step of selecting a tender stem segment with axillary buds or a semi-lignified stem segment, disinfecting the stem segment, cutting the stem segment into stem segments the length of which is 1.0-1.5 cm, and inoculating the stem segments to a culture medium with the temperature of a culture room being 23-25 DEG C; a step of multiplication culture, namely a step of cutting off blocks with buds after inoculation when the sprouts of the axillary buds are 1.0-2.0 cm tall, inoculating the blocks with buds to a differential medium and performing multiplication culture; a step of root induction culture in which robust test-tube single plantlets having height of 1-3 cm are selected and a 1 / 3MS culture medium is adopted as a base culture medium; and a step of plantlet hardening and transplanting, namely a step of, after developed roots of the test-tube single plantlets are formed, opening a bottle for hardening the plantlets for 5-8 days, taking the plantlets out, then cleaning, disinfecting and transplanting the plantlets to a cultivation medium. The method preserves excellent characters of koelreuteria paniculata and seedling uniformity, reduces dependence on a plurality of natural factors, has an advantage of a capability of rapidly breeding excellent varieties in a large scale, and has a propagation coefficient three or more times as much as the propagation coefficient of traditional seedling production.

Description

[0001] Technical field: [0002] The invention relates to the technical field of raising seedlings of eucalyptus, in particular to a breeding method of eucalyptus. [0003] Background technique: [0004] Luan tree (Koelreuteria paniculata), alias: Mu Luan, Luan Hua, etc., is a plant of Sapindaceae and the genus Luan. It is a deciduous tree or shrub; the bark is thick, gray-brown to gray-black, longitudinally cracked when old; the lenticels are small, gray to dark in color; the branchlets have warts, and the leaf shafts and petioles are covered with wrinkled pubescence or hairless. In the prior art, the seedlings of eucalyptus all adopt seeds to sow and raise seedlings. Because the seed coats of eucalyptus seeds are hard and difficult to permeate, if they are not managed by accelerating germination, they often do not germinate or the germination rate is very low in the second year of spring sowing. The germination rate is low, and the amount of seeds should be large, generally...

AI Technical Summary

Problems solved by technology

Therefore, there are many problems in the existing methods, such as long breeding period, low seed germination rate, large variation of offspring, and limitation of reproduction by natural conditions.