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Elastin peptide as well as preparation method and application thereof

A technology of elastin peptide and protease, which is applied in chemical instruments and methods, animal/human protein, specific peptides, etc., can solve the problems that are difficult to be used, and achieve the effects of low production cost, obvious anti-wrinkle effect, and strong anti-aging activity

Inactive Publication Date: 2017-02-08
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, elastin has a stable structure and is insoluble in solvents such as dilute acid, dilute alkali, water and ethanol, so it is difficult to be used

Method used

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  • Elastin peptide as well as preparation method and application thereof
  • Elastin peptide as well as preparation method and application thereof
  • Elastin peptide as well as preparation method and application thereof

Examples

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Effect test

preparation example Construction

[0033] A preparation method of elastin peptide with cosmetic efficacy, comprising the following steps:

[0034] (1) Preparation of elastin peptide: Wash bovine cervical ligament, add 0.1M NaOH solution 2-3 times its mass, soak at 40-50°C for 1-2 hours, remove surface fat and impurities, wash with water until neutral Drain and mince. Add demineralized water, adjust the pH value of the system to 7.0, stir and heat to 50-60°C, add protease at 50-60°C and keep warm for 10-20 hours, inactivate the enzyme, centrifuge and filter to get the supernatant.

[0035] (2) Concentrate the supernatant in vacuum to a solid content of about 35-45%, and spray-dry to obtain elastin polypeptide.

[0036] As preferably, in step (1), adding water is to add the water of 2~3 times of the mass of bovine cervical ligament minced meat;

[0037] As preferably, in step (1), the pH value of the adjustment system is adjusted with 0.5mol / L NaOH solution or HCl solution;

[0038] As a preference, in step (1...

Embodiment 1

[0070] A preparation method of elastin peptide with cosmetic efficacy, comprising the following steps:

[0071] (1) Wash the bovine cervical ligament, add 2 times the mass of 0.1M NaOH solution, soak for 1 hour at 50°C to remove surface fat and impurities, wash with water until neutral, drain and mince.

[0072] (2) Add 2 times of water, adjust the pH value of the system to 7.0, stir and heat in a water bath to 50° C., add Neutrase 0.8L (Novozymes) with 1.0% quality of bovine neck ligament meat paste and 0.4% bovine neck ligament meat paste quality Protamex ( Novozymes), incubate at 50°C for 20 hours, heat at 95°C for 15min to inactivate the enzyme, centrifuge at 5000r / min for 15min and pass through 500 mesh silk cloth for separation, take the permeate to obtain elastin peptide liquid.

[0073] (3) Vacuum-concentrate the elastin peptide solution to a solid content of about 35%, and spray-dry to obtain elastin peptide A.

[0074] The antioxidant value of elastin peptide A (red...

Embodiment 2

[0079] A preparation method of bovine neck ligament elastin peptide with cosmetic effect, comprising the following steps:

[0080] (1) Wash the bovine cervical ligament, add 3 times the mass of 0.1M NaOH solution, soak for 1 hour at 40°C to remove surface fat and impurities, wash with water until neutral, drain and mince.

[0081](2) Add 3 times of water, adjust the pH value of the system to 7.0, stir and heat in a water bath to 60°C, add Alphalase NP 0.8L (Genencor) with a mass of 0.5% minced beef neck ligament meat and 0.8% Protamex with a mass of beef neck ligament mince (Novozymes), heat at 60°C for 10 hours, heat at 95°C for 15min to inactivate the enzyme, centrifuge at 5000r / min for 15min and pass through 500 mesh silk cloth for separation, take the permeate to obtain elastin peptide liquid.

[0082] (3) Vacuum-concentrate the elastin peptide solution to a solid content of about 45%, and spray-dry to obtain elastin peptide B.

[0083] Antioxidant value of elastin peptid...

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Abstract

The invention discloses an elastin peptide with a beautifying effect as well as a preparation method and application thereof. The preparation method of the elastin peptide comprises the following steps: after pre-treating cattle neck ligaments, mixing the cattle neck ligaments with water; after adjusting the pH (Potential of Hydrogen) value, mixing the cattle neck ligaments with proteinase and carrying out enzymolysis; carrying out enzyme denaturing, centrifuging, filtering and collecting filtrate; taking the filtrate, concentrating and drying to obtain the elastin peptide. The method provided by the invention utilizes a biological enzymolysis technology, and a bioactive peptide with the beautifying effect is released from elastin of the cattle neck ligaments. The cattle neck ligament elastin peptide prepared by adopting the method has remarkable anti-wrinkle and moisturizing effects, can remarkably improve the moisture content in skins and the content of hyaluronic acid in blood serum and can also remarkably increase the content of collagen and the elastin in the skins.

Description

technical field [0001] The invention relates to the field of deep processing of elastin, in particular to an elastin peptide and its preparation method and application. Background technique [0002] Elastin is an important structural protein in human tissues and organs (such as arteries, lungs, skin). elasticity. Fibroblasts in the skin can synthesize and secrete elastin before puberty, but almost no longer synthesize elastin after puberty. As we grow older, elastase in the skin is activated, the balance between elastase and elastase inhibitors is broken, and elastin is continuously degraded, so the skin gradually appears sagging and wrinkles. [0003] In addition, 75% of the water in the skin is stored in the dermis, and the collagen fibers and elastic fibers formed by collagen and elastin are important components of the intercellular matrix in the dermis, which is full of rich mucopolysaccharides and proteins Complexes, such as hyaluronic acid, are important for keeping...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78A61K8/64A61Q19/08A23L33/18
CPCA61Q19/08A61K8/64C12P21/06C07K14/78
Inventor 丁刘刚梁明郭晓蕾马忠华张婷苏国万
Owner INFINITUS (CHINA) CO LTD
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