Kit for detecting high density lipoprotein cholesterol

Abstract

The invention discloses a kit for detecting high density lipoprotein cholesterol. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution I, 0.1-10 mmol / L polyanionic compound, 0.1-100 mmol / L bivalent cation, 0.1-10 mmol / L developer, 1-100 g / L surfactant I, 1-100 g / L surfactant II and 1-50 g / L preservative agent; and the reagent R2 comprises the following components of the corresponding concentrations: 10-100 mmol / L buffer solution II, 0.1-10 mmol / L 4-aminoantipyrine, 1-100 g / L surfactant III, 0.1-10 KU / L cholesterase, 0.1-10 KU / L cholesterol oxidase, 0.1-10 KU / L peroxidase and 1-50 g / L preservative agent. According to the invention, improvement is made on the basis of a selective inhibition method; the preferable compound surfactant pairs are added to inhibit and eliminate lipemia interference in the first reaction step, so that lipemia samples can be directly detected by a full-automatic biochemical analysis instrument without being pretreated; and the kit is simple to operate, improves the detection efficiency and lowers the detection cost.

Description

technical field [0001] The invention relates to a kit for detecting high-density lipoprotein cholesterol, which belongs to the field of high-density lipoprotein cholesterol measurement. Background technique [0002] The existence and transport form of cholesterol in human plasma is plasma lipoprotein, that is, non-polar lipids including cholesterol and hydrophilic apolipoprotein binding. Plasma lipoproteins can be divided into four main types according to their density: high-density lipoprotein (HDL), low-density lipoprotein (LDL), very low-density lipoprotein (VLDL) and chylomicrons (CM). Among them, high-density lipoprotein is composed of various protein-rich particles. It is a group of heterogeneous lipoproteins and is the lipoprotein with the highest density (d=1.063~1.210kg / L). Among its components, protein (Pro), phospholipid (PL), cholesterol (CHO) and triglyceride (TG) account for about 50%, 25%, 20% and 5% respectively. The main proteins in HDL are apolipoproteins...

AI Technical Summary

Problems solved by technology

Among them, the selective inhibition method is widely used clinically. This method has the advantages of good accuracy, high precision, and wide linear range, but it has defects in antilipidemia interference.

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