A mesenchymal stem cell cryoprotectant

Abstract

A mesenchymal stem cell cryoprotectant is disclosed. Raw materials of the cryoprotectant includes, by volume, 65.5-75.4% of 1640 culture liquid, 9.8-10.1% of dimethyl sulfoxide, 9.8-11.2% of human albumin, 9.9-10.8% of hydroxyethyl starch and 0.5-1.5% of a fibroblast growth factor. The cryoprotectant is more suitable for cryopreservation of mesenchymal stem cells, the anchoring rate of the mesenchymal stem cells after resuscitation reaches 90% or above, dead cells are few, and the quantity of cells obtained after culturing for 7 days is 2*10<7> per flask, thus greatly increasing the quantity of cells. During cell phenotype detection, CD44<+> is 99% or above and CD105<+> is 99% or above.

Description

technical field [0001] The invention relates to the technical field of cryopreservation solution, in particular to a mesenchymal stem cell cryopreservation solution. Background technique [0002] At present, the method of using the stem cell cryopreservation solution is: after the cell culture reaches a certain density, it needs to be frozen and stored in liquid nitrogen to maintain the activity of the cells, and it can be cultured normally after recovery in the future, so it is necessary to add a certain proportion when freezing. The cryopreservation solution keeps the cells intact and minimizes the damage to the cells during cryopreservation. [0003] The cell cryopreservation solution in the prior art is often obtained by mixing fetal bovine serum and dimethyl sulfoxide at a volume ratio of 9:1. Due to the use of animal-derived fetal bovine serum, the cell cryopreservation solution is mixed with non-human source substance, causing residue or pollution, and the existing c...

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Problems solved by technology

[0003] The cell cryopreservation solution in the prior art is often obtained by mixing fetal bovine serum and dimethyl sulfoxide at a volume ratio of 9:1. Due to the use of animal-derived fetal bovine serum, the cell cryopreservation solution is mixed with non-human source substance, causing residue or pollution, and the existing cell cryopreservation medium has a stem cell adhesion rate of about 80% during the subsequent recovery and culture of stem cells, and there are many dead cells, and the final cell number obtained is 1.2×10 7 / bottle or so, the cost is also higher

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