Fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops

A technology for Bacillus atrophicus and cruciferous crops is applied in the field of fermentation of Bacillus atrophicus BA-7, which can solve problems such as limiting the practical application of biocontrol strains, and achieve good inhibition and killing effect, good control effect, and rapid decay. Effect
CN106399150AActive Publication Date: 2017-02-15INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
Publication Date
2017-02-15

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Abstract

The invention provides a fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops. The fermentation method comprises the following steps: (1) culturing the bacillus atrophaeus BA-7 in a solid medium, picking single colonies, placing the single colonies in a fluid medium, carrying out shake culture for 10-12 h at the temperature of 30-32 DEG C, thus obtaining the seed solution, wherein the rotation speed is 200-220 rpm; (2) after inoculation, carrying out fermentation for 24-48 h, wherein the temperature of the tank is 28-32 DEG C, the stirring speed of the fermentation tank is 220-250 rpm, the culture medium in the fermentation tank is prepared from the following components in percentage by weight: 4-5 % of soya flour, 0.1-0.15 % of peptone, 0.5-0.7 % of glucose, 2-3 % of starch, 0.1% of manganese sulfate, 0.06% of ferrous sulfate, 0.2% of dipotassium phosphate, 0.1% of calcium carbonate, 0.01% of a defoamer, the balance of water, and the pH is 7.0-7.4. The classification name of the bacillus atrophaeus is bacillus atrophaeus BA-7, and the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection (CCTCC), and is assigned with the accession number of CCTCC NO:M 2015765.
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Description

technical field

[0001] The invention belongs to the technical field of biopesticides, and in particular relates to a fermentation method of bacillus atrophaeus BA-7 for preventing and treating clubroot of cruciferous crops. Background technique

[0002] In the prior art, the biocontrol bacterial strains that are used to control cruciferous crop clubroot are seldom, mainly concentrate on Bacillus subtilis (such as Bacillus subtilis XF-1 and Bacillus subtilis M3) and Bacillus amyloliquefaciens (such as amyloliquefaciens Bacillus Bam22), which greatly limits the practical application of biocontrol strains in the control of clubroot in cruciferous crops. Importantly, researchers are still working hard to explore whether there are other corresponding biocontrol bacteria.

[0003] The inventor of the present invention has successfully isolated a strain of Bacillus atrophaeus BA-7 which has a very good control effect on cruciferous crops (especially rapeseed) after a long period o...

Claims

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