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Fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops

A technology for Bacillus atrophicus and cruciferous crops is applied in the field of fermentation of Bacillus atrophicus BA-7, which can solve problems such as limiting the practical application of biocontrol strains, and achieve good inhibition and killing effect, good control effect, and rapid decay. Effect

Active Publication Date: 2017-02-15
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] In the prior art, the biocontrol bacterial strains that are used to control cruciferous crop clubroot are seldom, mainly concentrate on Bacillus subtilis (such as Bacillus subtilis XF-1 and Bacillus subtilis M3) and Bacillus amyloliquefaciens (such as amyloliquefaciens Bacillus Bam22), which greatly limits the practical application of biocontrol strains in the control of clubroot in cruciferous crops

Method used

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  • Fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops
  • Fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops

Examples

Experimental program
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Effect test

Embodiment 1

[0023] 1. Culture of strains

[0024] (1) After culturing Bacillus atrophaeus BA-7 on a solid medium, pick a single colony, place it in a liquid medium, and cultivate it with shaking at 30-32° C. for 12 hours, with a rotating speed of 220 rpm, as a seed liquid; the liquid The components of the medium include: 0.3% of yeast extract, 0.2% of beef extract, 0.8% of peptone, 0.3-0.6% of NaCl, the balance is water, pH=7.0-7.2;

[0025] (2) Put the product of step (1) into a fermenter to ferment for 24 hours, the tank temperature is 30° C., and the stirring speed of the fermenter is 250 rpm; the composition of the culture medium in the fermenter includes by mass percentage: soybean powder 4.5% , peptone 0.12%, glucose 0.6%, starch 2.5%, manganese sulfate 0.1%, ferrous sulfate 0.06%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1%, defoamer 0.01%, the balance is water, pH=7.0 -7.4.

[0026] 2. Biocontrol effect experiment

[0027] Dilute the culture obtained in the abov...

Embodiment 2

[0041] In terms of bacterial strain cultivation, except for step (1), the liquid medium contains 0.1% yeast extract, 0.1% beef extract, 0.6% peptone, 0.6% NaCl, and the balance is water, pH=7.0-7.2; fermentation medium The ingredients are soybean powder 4%, peptone 0.1%, glucose 0.5%, starch 2%, manganese sulfate 0.1%, ferrous sulfate 0.06%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1%, defoamer 0.01%, and The amount is water, pH=7.0-7.4; the fermentation temperature is 28° C., and the fermentation time is 48 hours; the rest are consistent with Example 1. The concentration of the obtained bacterial solution is 4-5×10 9 cfu / ml.

Embodiment 3

[0043] In terms of bacterial strain cultivation, except for step (1), the liquid medium contains 0.1-0.4% of yeast extract, 0.3% of beef extract, 1.0% of peptone, 0.3% of NaCl, and the balance is water, pH=7.0-7.2; The medium components are soybean powder 5%, peptone 0.15%, glucose 0.7%, starch 3%, manganese sulfate 0.1%, ferrous sulfate 0.06%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1%, defoamer 0.01%, The balance is water, pH=7.0-7.4; the fermentation temperature is 32° C., and the fermentation time is 36 hours; the rest is consistent with Example 1. The concentration of the obtained bacterial solution is 7-8×10 8 cfu / ml.

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Abstract

The invention provides a fermentation method of bacillus atrophaeus BA-7 capable of preventing and treating clubroot of cruciferae crops. The fermentation method comprises the following steps: (1) culturing the bacillus atrophaeus BA-7 in a solid medium, picking single colonies, placing the single colonies in a fluid medium, carrying out shake culture for 10-12 h at the temperature of 30-32 DEG C, thus obtaining the seed solution, wherein the rotation speed is 200-220 rpm; (2) after inoculation, carrying out fermentation for 24-48 h, wherein the temperature of the tank is 28-32 DEG C, the stirring speed of the fermentation tank is 220-250 rpm, the culture medium in the fermentation tank is prepared from the following components in percentage by weight: 4-5 % of soya flour, 0.1-0.15 % of peptone, 0.5-0.7 % of glucose, 2-3 % of starch, 0.1% of manganese sulfate, 0.06% of ferrous sulfate, 0.2% of dipotassium phosphate, 0.1% of calcium carbonate, 0.01% of a defoamer, the balance of water, and the pH is 7.0-7.4. The classification name of the bacillus atrophaeus is bacillus atrophaeus BA-7, and the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection (CCTCC), and is assigned with the accession number of CCTCC NO:M 2015765.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a fermentation method of bacillus atrophaeus BA-7 for preventing and treating clubroot of cruciferous crops. Background technique [0002] In the prior art, the biocontrol bacterial strains that are used to control cruciferous crop clubroot are seldom, mainly concentrate on Bacillus subtilis (such as Bacillus subtilis XF-1 and Bacillus subtilis M3) and Bacillus amyloliquefaciens (such as amyloliquefaciens Bacillus Bam22), which greatly limits the practical application of biocontrol strains in the control of clubroot in cruciferous crops. Importantly, researchers are still working hard to explore whether there are other corresponding biocontrol bacteria. [0003] The inventor of the present invention has successfully isolated a strain of Bacillus atrophaeus BA-7 which has a very good control effect on cruciferous crops (especially rapeseed) after a long period o...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01P3/00C12R1/07
CPCC12N1/20
Inventor 刘勇伍文宪黄小琴张蕾尹全刘红雨周西全
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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