A molecular identification method and application of Dendrobium candidum dry medicinal material
A technology of Dendrobium officinale and medicinal materials, which is applied in biochemical equipment and methods, microbiological measurement/inspection, DNA preparation, etc., can solve the problems of long time, low amplification success rate, sample DNA degradation, etc., and achieve high accuracy, Widely applicable, easy-to-master effects
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Embodiment 1
[0020] The identification of embodiment 1 Dendrobium candidum medical material
[0021] 1. Experimental materials
[0022] In this case, a total of 173 samples of 40 species of Dendrobium were collected, and 3 samples of fake Dendrobium dendrobii and Dendrobium fringe were collected. Among them, there are 14 original plant samples of Tiepi Fengdouji and 65 samples of medicinal materials. The specific information is shown in Table 1.
[0023] Table 1 Sample information table of Tiepi Fengdou and its counterfeit products
[0024]
[0025]
[0026]
[0027] 2. Experimental equipment
[0028] Desktop high-speed centrifuge, PTC-100 gene amplification instrument, electrophoresis instrument, ultraviolet gel imaging analysis system, MM400 ball mill (Germany Retsch).
[0029] 3. DNA extraction
[0030] Take 20-30 mg of dried Tiepi Fengdou herbs, chop them up and crush them with MM400 ball mill, add 500-1000 μL of nuclear separation solution, mix well, centrifuge at 7000 r / ...
Embodiment 2
[0042] The identification of embodiment 2 Dendrobium officinale base plant
[0043] Basically the same as Example 1, except that the original plant of Dendrobium candidum was used as the sample, DNA was extracted according to the above method, and the ITS2 sequence was amplified. As a result, the target sequence was also successfully obtained and the identification was completed.
Embodiment 3
[0044] The identification of embodiment 3 dendrobium officinale seed seedlings
[0045] Basically the same as Example 1, the difference is that Dendrobium officinale seeds and seedlings are used as samples, DNA is extracted according to the above method, and the ITS2 sequence is amplified. As a result, the target sequence can also be successfully obtained and the identification is completed.
[0046]
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