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Tumor therapy medicine targeting SCNN1A and application

A drug and tumor technology, applied in anti-tumor drugs, gene therapy, drug combination, etc., can solve problems such as unsatisfactory treatment effect and prognosis, and endanger human health

Inactive Publication Date: 2017-02-22
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Tumor is a killer that endangers human health. At present, the main treatment methods are surgical resection, radiotherapy and chemotherapy, but the treatment effect and prognosis are not ideal

Method used

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  • Tumor therapy medicine targeting SCNN1A and application
  • Tumor therapy medicine targeting SCNN1A and application
  • Tumor therapy medicine targeting SCNN1A and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Expression Analysis of SCNN1A in Tumor Tissue

[0028] PCR technology was used to detect the expression of SCNN1A in human colon cancer and its adjacent cancer, liver cancer and its adjacent cancer, lung cancer and its adjacent cancer. The cases in each group included 355 cases of colon cancer tissue, 19 cases of colon cancer tissue and 13 cases of liver cancer tissue. , 7 cases of liver cancer tissue; 112 cases of lung cancer tissue, 10 cases of lung cancer tissue. The results showed that the expression of SCNN1A in colon cancer, liver cancer, and lung cancer tissues was significantly lower than that in adjacent tissues, such as figure 1 a.

[0029] Further verification in human liver cancer cells: extract the mRNA of human normal liver cell line LO2, liver cancer cell lines Hep3B, Bel7407, Huh7, HepG2, and use real-time fluorescent quantitative PCR to detect the expression level of SCNN1A, the results show that Hep3B, Bel7407, Huh7 and other cell lines The...

Embodiment 2

[0033] Example 2 In vitro inhibitory effect of SCNN1A on liver cancer cells Hepa1-6

[0034] The cDNA sequence of the murine SCNN1A gene was constructed into the eukaryotic overexpression backbone vector pcDNA3.1, and the Hepa1-6 cell line was transfected by liposome method, and the pcDNA3.1 empty vector was transfected at the same time as a control. G418 screened to obtain resistant clones, and then detected the expression of SCNN1A by real-time quantitative PCR, and selected SCNN1A-pcDNA3.1#1 clones with similar expression levels of SCNN1A in liver tissue and pcDNA3.1#1 with similar expression levels to Hepa1-6 for in vitro experiments . Among them, quantitative results such as figure 2 a.

[0035] MTT, cell scratch, colony and flow cytometry were performed on Hepa1-6, pcDNA3.1-Hepa#1 (referred to as pcDNA3.1), SCNN1A-pcDNA3.1-Hepa#1 (referred to as SCNN1A-pcDNA3.1) cell lines respectively The cycle assay detects the growth inhibition of SCNN1A on the Hepa1-6 cell line. ...

Embodiment 3

[0040] Example 3 Subcutaneous tumor-bearing results of nude mice overexpressing Hepa1-6 in SCNN1A

[0041] 15 male nude mice were randomly divided into 3 groups, 5 in each group, and each mouse was inoculated with 6×10 6 The three groups of cell lines SCNN1A-pcDNA3.1, pcDNA3.1, and Hepa1-6 were inoculated subcutaneously in the left upper limb of nude mice respectively. Tumor formation was observed every day after inoculation. From the 7th day after injection, the tumor volume was measured according to the prescribed time. A tumor with a diameter of 3 mm was regarded as a tumor, and the tumor size was recorded. The longest and shortest diameters of the transplanted tumors were measured with a vernier caliper, once every 3 days, and the tumor volume was calculated according to the formula "tumor volume V = long diameter × short diameter × short diameter / 2". According to the average tumor volume, the tumor growth curve was drawn. On the 15th day, the nude mice were sacrificed...

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Abstract

The invention relates to tumor therapy medicine targeting SCNN1A and application and belongs to the technical field of medicine. On one hand, the medicine for effectively treating tumors is provided and can target molecular SCNN1A; on the other hand, the invention provides application of SCNN1A gene medicine and / or SCNN1A agonist in tumor treatment. A new conception is provided for tumor treatment.

Description

technical field [0001] The invention belongs to the technical field of drugs, and in particular relates to a drug for treating tumors targeting SCNN1A and its application. Background technique [0002] Tumor is a killer that endangers human health. At present, the main treatment methods are surgical resection, radiotherapy and chemotherapy, but the treatment effect and prognosis are not ideal. Molecular targeted drugs are a new treatment method, mainly because the occurrence and development of tumors is a process involving multiple steps and multiple genes. A series of oncogenes and tumor suppressor genes (β-catenin, Forkhead Box, p53, etc.) are dysregulated in tumors, and participate in the malignant transformation of tumors through various signaling pathways such as promoting cell proliferation, enhancing cell invasion and migration, and inhibiting cell apoptosis. These abnormally expressed genes are potential target molecules for the treatment of tumors. [0003] ENaC i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K48/00A61K38/17A61K31/137A61P35/00
CPCA61K31/137A61K38/1709A61K45/00A61K48/0058
Inventor 余陈欢张欢欢方杰马月陈勤应华忠
Owner ZHEJIANG ACAD OF MEDICAL SCI