Method for extracting DNA of fertilized egg genome of ascaris suum

Abstract

The invention provides a method for efficiently extracting DNA of a fertilized egg genome of ascaris suum. The method comprises the following five steps: washing eggs with distilled water; pre-treating the eggs; digesting protease K; removing impurities; and eluting DNA with high-purity water. The extracted DNA of the fertilized egg genome of ascaris suum has stable quality and can be used for effectively performing downstream PCR amplification, and an important technical method is provided to studying of molecular epidemiology, genetic diversity, genetic structure, systemic embryology and phylogeography of ascaris suum. The method has an important meaning to molecular diagnosis, prevention and control on porcine ascariasis.

Description

technical field

[0001] The invention relates to the field of biotechnology, and relates to a method for extracting genomic DNA from fertilized eggs of Ascaris suum. Background technique

[0002] Ascaris suum ( Ascaris suum Goeze, 1782) belongs to the phylum Nematodes, class Caudosensilla, order Ascaria, and genus Ascaris. It is the largest nematode parasitic in the small intestine of pigs, and the fresh worm body is light red or light yellow. The development of Ascaris suum includes three different developmental stages: eggs, larvae, and adults. The newly hatched eggs belong to the single-cell stage and are discharged to the outside with the feces. , forming infective eggs (ie third stage larvae). After the infectious eggs are swallowed by pigs, larvae hatch in the small intestine. Most of the larvae enter the blood vessels through the intestinal wall, enter the liver with the blood flow, and then migrate to the lungs. Finally, they undergo two molts in the small intesti...

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