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Kit for detecting homocysteine

A homocysteine ​​and kit technology, which is applied in the field of homocysteine ​​detection kits, can solve the problems of cumbersome operation and long time consumption, and achieve the effects of improving sensitivity, fast speed and wide application range

Inactive Publication Date: 2017-02-22
BEIJING 3S CENTURY TECH CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But the operation is cumbersome and time-consuming

Method used

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  • Kit for detecting homocysteine
  • Kit for detecting homocysteine
  • Kit for detecting homocysteine

Examples

Experimental program
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Effect test

Embodiment 1

[0066] The homocysteine ​​kit consists of the following R1 and R2, and the pH value after mixing is 8.0-8.5.

[0067] 1. The concentration and name of each component of R1 are shown in Table 1.

[0068] Table 1 Concentration and name of each component of R1

[0069]

[0070] 2. The concentration and name of each component of R2 are shown in Table 2.

[0071] Table 2 Concentration and name of each component of R2

[0072]

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Abstract

The invention discloses a kit for detecting homocysteine. The kit comprises a reagent R1 and a reagent R2, wherein the reagent R1 contains the following components and concentrations: 10-200mmol / L of buffer solution, 1-10mmol / L of homocysteine reducing agent, 0.1-15mmol / L of serine, 0.2-5.8mg / L of coenzyme, 5-100KU / L of lactic dehydrogenase, 0.01-0.8g / L of reducing coenzyme I, 5-150g / L of enzyme stabilizer, 1-100g / L of surface active agent and 0.1-5g / L of preservative; the reagent R2 contains the following components and concentrations: 10-200mmol / l of buffer solution, 0.2-5.8mg / L of coenzyme, 1-100KU / L of cystathionine beta-synthetase, 1-100KU / L of cystathionine beta-lyase, 5-150g / L of enzyme stabilizer, 1-100g / L of surface active agent and 0.1-5g / L of preservative. The kit disclosed by the invention has the advantages that the use amount of enzyme can be reduced, the stability of detection can be improved, the sensitivity of cyclic detection can be improved, the accuracy is high and the application range is wide. The method is good in correlation with an HPLC (High Performance Liquid Chromatography) method, pretreatment for a sample is not needed, the detection on a full-automatic biochemical analyzer is automatic and the speed is high.

Description

technical field [0001] The invention relates to a kit for detecting homocysteine. Background technique [0002] Homocysteine ​​(HCY) is a sulfur-containing amino acid and a metabolite of methionine. Recent studies have found that HCY can cause birth defects such as neural tube defects (NTDs) and congenital heart defects (CHD), and HCY is also closely related to the pathogenesis of atherosclerosis. [0003] As early as 40 years ago, Dr. McCully first discovered and discussed the relationship between homocysteine ​​and the occurrence and development of cardiovascular diseases. Patients with homocystinuria are often in their teens to twenties. Severe cardiovascular disease occurs in adolescence. Recent studies have found that 10-20% of patients with cardiovascular disease are not related to risk factors such as cholesterol levels, smoking and high blood pressure, but are caused by elevated homocysteine. Genetics and eating habits are the main reasons for the increase of homo...

Claims

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Application Information

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IPC IPC(8): C12Q1/527C12Q1/32
Inventor 黄妍刘学仁江水
Owner BEIJING 3S CENTURY TECH CORP
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