Quick detection method for mastitis

Abstract

The invention discloses a quick detection method for mastitis. The method comprises the following steps that genomic DNA to be detected is extracted; a mutiplex PCR reaction system is set; a designed primer group is added into the mutiplex PCR reaction system for reacting, and the reaction procedures that predegeneration is conducted for 5 min at 94 DEG C, degeneration is conducted for 45 s at 94 DEG C, annealing is conducted for 30 s at 57 DEG C, and extending is conducted for 90 s at 72 DEG C are included and conducted 32 cycles; extending is conducted for 10 min at 72 DEG C, and then a mutiplex PCR amplification product is obtained; gel electrophoresis is conducted on the mutiplex PCR amplification product, and whether the sample to be detected is subjected to single infection or mixed infection of staphylococcus aureus, streptococcus agalactiae, escherichia coli and mycoplasma bovis or not is judged according to electrophoretic bands generated in the sample to be detected. According to the method, the good sensitivity and specificity are achieved, four kinds of cow mastitis caused by the staphylococcus aureus, the streptococcus agalactiae, the escherichia coli and the mycoplasma bovis can be detected simultaneously, and then the cow mastitis is quickly and effectively prevented and controlled.

Description

technical field [0001] The invention relates to the technical field of dairy cow molecular biology, in particular to a rapid detection method for mastitis. Background technique [0002] For decades, many efforts have been made to prevent and treat dairy cow mastitis at home and abroad, but the disease is still one of the diseases that cause the most serious loss in dairy farms. At present, there is still a lack of effective vaccines to prevent mastitis in dairy cows in China. The key factor in the prevention and treatment of this disease is to identify its causative bacteria. Once the pathogenic bacteria are identified, corresponding measures can be taken immediately. [0003] The research found that Staphylococcus aureus, Streptococcus agalactiae, Mycoplasma bovis and Escherichia coli are the four most important pathogens causing mastitis in dairy cows. The multiplex PCR rapid diagnosis method is applied to mycoplasma without cell wall and other pathogenic bacteria simult...

AI Technical Summary

Problems solved by technology

There has been no similar report on the simultaneous application of multiplex PCR rapid diagnostic methods to mycoplasma and other pathogenic bacteria without cell walls

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