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Rapid production method of paper-based microfluidic chip

A technology of a microfluidic chip and a manufacturing method, which is applied in the directions of analyzing materials and instruments, can solve the problems of easy clogging of the pen tip, affecting the detection, discontinuous hydrophobic boundary of the chip, etc., and achieves a fast and simple manufacturing process, cost reduction, and easy availability of materials. Effect

Inactive Publication Date: 2017-02-22
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this technical method is that the pen tip is easy to block during the drawing process, and the hydrophobic boundary of the chip produced according to this method is discontinuous, which affects subsequent detection

Method used

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  • Rapid production method of paper-based microfluidic chip
  • Rapid production method of paper-based microfluidic chip
  • Rapid production method of paper-based microfluidic chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Fabrication of paper-based microfluidic chips:

[0034] (1) Take an ordinary writing water pen and use a syringe to absorb the ink in the refill.

[0035] (2) Take 0.15g of vacuum silicone grease and add it to 12.5mL n-heptane, ultrasonically dissolve the vacuum silicone grease, then add 0.5g of alkyl ketene dimer (AKD) to the above solution, ultrasonically dissolve, and dissolve the The mixture is used as hydrophobic agent. Use a syringe to draw 0.1 mL of the above hydrophobic agent solution and inject it into a modified pen.

[0036] (3) Use the modified pen above to make a mold with an orifice plate with a diameter of 8mm, draw the pattern directly on Whatman#1 filter paper, then put it in an oven, and dry it at 100°C for 3 minutes to make a paper-based microfluidic chip Successfully, the properties of this chip are characterized by Figure 1a , 1b and Figure 2a , as shown in 2b.

Embodiment 2

[0038] Fabrication of paper-based microfluidic chips:

[0039] (1) Take an ordinary writing water pen and use a syringe to absorb the ink in the refill.

[0040] (2) Add 0.15g of vacuum silicone grease to 12.5mL of n-hexane, ultrasonically dissolve the vacuum silicone grease, then add 0.5g of paraffin wax to the above solution, ultrasonically dissolve, and use this mixture as a hydrophobic agent. Draw 0.1 mL with a syringe and inject into a modified fountain pen.

[0041] (3) Use the above-mentioned modified pen to draw patterns directly on the paper, and then dry it with a hot blower until the solvent is completely evaporated, and the paper-based microfluidic chip is successfully fabricated.

Embodiment 3

[0043] Paper-based microfluidic chip for the detection of uric acid samples:

[0044] (1) Take uric acid detection reagents uric acid oxidase (80U / mL), horseradish peroxidase (340U / mL), 4-aminoantipyrine (4mM / L), 3,5-dichloro-2- 1 μL of sodium hydroxybenzenesulfonate (8 mM / L) was sequentially added dropwise to the center of the detection area, and dried.

[0045] (2) Add uric acid standard solutions with concentrations of 0, 15, 35, 50, 75, and 100 ppm respectively and the processed serum samples S1 and S2 dropwise to the central positions of different detection areas, and dry.

[0046] (3) Use a mobile phone to take pictures of the detection area of ​​the paper chip. The photos are as follows: image 3 As shown, and the photo is processed with ImageJ software to obtain the gray value, the gray value is the ordinate, and the concentration of each solution is the abscissa to make a standard curve, such as Figure 4 shown. Substituting the gray value of the measured sample, t...

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Abstract

The present invention discloses a rapid production method of a paper-based microfluidic chip, wherein a hydrophobic material solution is filled in the empty pen cartridge of conventional perfusable writing pens, drawing pens and the like, the required pattern is directly drawn on paper or is drawn by combining the pen and a template according to the actual determination demand, and different configurations of hydrophilic and hydrophobic areas are formed after drying so as to obtain the paper-based microfluidic chip capable of achieving multiple detection functions. According to the present invention, the production method has characteristics of simpleness, rapidness, low cost, good stability and the like, and the paper-based microfluidic chip is particularly suitable for being used in the conventional laboratories or underdeveloped areas, and is expected to be used for medical diagnosis, environmental monitoring, and rapid food quality detection.

Description

[0001] 【Technical field】 [0002] The invention relates to the technical field of microfluidic chips, in particular to a rapid manufacturing method for paper-based microfluidic chips. [0003] 【Background technique】 [0004] The traditional chemical or biological experiment process is cumbersome and complicated, and usually requires several different instruments to cooperate with each other to complete the entire experimental process, and the emergence of microfluidic chips has solved this problem well. Microfluidic chip refers to the integration of basic operating units such as sample preparation, biological and chemical reactions, and separation and detection involved in the fields of biology and chemistry into the chip, which is used to complete different biological or chemical reaction processes and analyze the products. for analysis. The substrate materials for making chips include monocrystalline silicon wafers, quartz glass, high molecular polymers, etc. Paper-based ch...

Claims

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Application Information

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IPC IPC(8): G01N35/00
Inventor 胡坪赵甜甜张敏王月荣章弘扬
Owner EAST CHINA UNIV OF SCI & TECH
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