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Bioassay system and method for detecting analytes in body fluids

A technology for biological assay and analyte, which is applied in the field of detecting analytes in body fluids and detecting analytes in body fluids, and can solve problems such as success limitation

Active Publication Date: 2017-02-22
INSTR LAB
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Problems solved by technology

One approach for improving upconversion luminescence is to chemically modify the nanoparticle surface, but this approach has had limited success

Method used

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  • Bioassay system and method for detecting analytes in body fluids
  • Bioassay system and method for detecting analytes in body fluids
  • Bioassay system and method for detecting analytes in body fluids

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Embodiment Construction

[0018] One way to increase the sensitivity of bioassays is to enhance the luminescence by using polymer-based grating substrates, thereby increasing the light level irradiated, but this can lead to certain problems, such as high background and increased sample temperature and sample damage. For example, in such bioassay systems, polymer-based grating substrates start to exhibit excess background due to autofluorescence. Such interference from the background is unsuitable for high-sensitivity biosensing applications such as immunoassays. Although high-quality and high-purity quartz substrates have been used to solve this problem, these materials lead to prohibitive material costs and more complex manufacturing processes. Therefore, the present invention employs an upconversion nanoparticle that absorbs near-infrared excitation combined with a resonant grating structure to achieve high sensitivity in a biosensing platform for detecting analytes.

[0019] see Figure 1A and 1B ...

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Abstract

A biosensor platform is configured with upconverting nanoparticles on the surface of a resonant grating structure with enhanced sensitivity. The grating structure is illuminated with a light beam at one of its resonance modes to form a strong evanescent field at the surface and used for high sensitivity assays. The strong evanescent field triggers the upconverting nanoparticles to generate enhanced and localized emission on the grating surface, with lower background and lower auto-fluorescence from the grating substrate. This leads to improved performance in detecting analytes in bioassays.

Description

technical field [0001] The present invention relates to a bioassay system and a method for detecting analytes in body fluids. More specifically, the present invention relates to a bioassay device (such as an immunoassay device) comprising a resonant grating structure, and a device for detecting analytes in body fluids using upconverting nanoparticles and the resonant grating structure. method. Background technique [0002] Traditionally, fluorophores have been used as labels in bioassay devices. However, fluorophores undergo photobleaching over time. Various attempts have been made to use upconverting nanoparticles (i.e., particles between 1 nm and 100 nm in diameter and emitting light at wavelengths shorter than the excitation light wavelength) as labels in bioassay devices because upconverting nanoparticles do not Photobleaching occurs. However, the application of upconverting nanoparticles raises other issues. For example, the luminescence of upconverting nanoparticl...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65G01N21/77G01N33/543G01N33/58
CPCG01N21/658G01N21/7743G01N33/54373G01N33/588G01N33/54346
Inventor 约瑟夫·克里莫曾寒松罗恩·沙尔拉克格特·布兰肯施泰因
Owner INSTR LAB
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