Detoxication agent for zearalenone in biodegradable feedstuff and preparation process of detoxication agent

Abstract

The invention relates to a detoxication agent for zearalenone in biodegradable feedstuff and a preparation process. The detoxication agent comprises the following components: 40-60 parts of bentonite, 20 parts of immunopolysaccharide, 10-20 parts of a free-radical scavenger, and 20-30 parts of bacillus subtilis powder for degrading the zearalenone. A method for preparing the bacillus subtilis powder comprises the following steps: (1) carrying out seed solution activation on original strains, and carrying out mother culture in a primary fermenting tank containing a culture medium; (2) carrying out scale-up culture on mother culture liquid in a secondary fermenting tank containing an LB culture medium; and (3) separating thallus from fermentation liquor, concentrating and drying to obtain the bacillus subtilis powder for degrading the zearalenone. The powder is compounded with the two-time mixture in part by weight to obtain the finished product. The degradation rate of the zearalenone reaches up to 95%, the degradation efficiency is high, and defects of an adsorbent are avoided. In addition, the immunopolysaccharide and the free-radical scavenger are added in the product, therefore, functions of protecting the liver and improving the immunity of the organism can be fulfilled, and effects are obvious.

Description

technical field [0001] The invention belongs to feed additive technology, and more specifically relates to a detoxification agent for degrading zearalenone in animal feed through biotechnology and a preparation process of the detoxification agent. Background technique [0002] Feed mycotoxins: secondary metabolites produced by molds, which have negative effects on animal growth rate, body organs, reproductive performance, production performance, etc. [0003] Zearalenone: It has estrogenic effects, mainly acting on the reproductive system, and can cause hyperestrogenism in livestock and poultry. The consumption of zearalenone-containing feed by female animals (including humans) can cause miscarriage, stillbirth and teratogenicity; [0004] Mycotoxin adsorbent: absorb part, a small amount, and specific toxins physically, and excrete them from the body without changing the properties and chemical structure of the toxins; [0005] At present, the pollution of raw materials in...

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Problems solved by technology

However, due to the many types of mycotoxins in this method, the enzyme system is complex and complete, and it is easily affected by the function of the animal's digestive organs; in addition, due to the lack of heat resistance of the enzyme, it is easy to inactivate during the feed processing process, and the cost is high, and it is difficult to Promote the use in actual production

There are many reports on the use of microbial fermentation to remove mycotoxins, such as the fermentation of mycotoxins into other products, but this conversion is usually slow and incomplete, and the safety of the converted products is still inconclusive. To play a role in actual production, systematic and perfect research is needed

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