Detoxifying agent for biologically degrading ochratoxin A in feeds and preparation technology of detoxifying agent

Abstract

The invention relates to a detoxifying agent for biologically degrading ochratoxin A in feeds and a preparation technology of the detoxifying agent. The detoxifying agent comprises 40-60 parts of bentonite, 20 parts of immune polysaccharides, 10-20 parts of a free-radical scavenger and 20-30 parts of bacillus subtilis powder for degrading the ochratoxin A, wherein the bacillus subtilis powder is prepared through the following steps of (1) performing seed liquor activation on original strains, and performing mother seed culturing in a first-class fermentation tank loaded with culture mediums; (2) performing amplified culturing on mother seed culturing liquor in a second-class fermentation tank loaded with LB culture mediums; and (3) separating thalli from fermentation liquor, performing concentration, and performing drying so as to prepare the bacillus subtilis powder for biologically degrading the ochratoxin A. The bacillus subtilis powder, a first-time mixture and a second-time mixture in parts by weight are compounded so that a finished product of the detoxifying agent is obtained. According to the detoxifying agent disclosed by the invention, the degradation rate of the detoxifying agent for the ochratoxin A is as high as 92% or above, the degradation efficiency is high, and defects of an adsorbent are overcome; and besides, immune polysaccharide and free-radical removing ingredients are added to products, so that the functions of protecting the liver and enhancing the immunity of organisms can be achieved, and the effects are obvious.

Description

technical field [0001] The invention belongs to feed additive technology, and more specifically relates to a detoxification agent for degrading ochratoxin in animal feed through biotechnology and a preparation process of the detoxification agent. Background technique [0002] Feed mycotoxins: secondary metabolites produced by molds, which have negative effects on animal growth rate, body organs, reproductive performance, production performance, etc. [0003] Ochratoxin (Ochratoxin A, OA): is a toxic metabolite produced by Aspergillus and Penicillium, which has strong nephrotoxicity and liver toxicity, and has teratogenic, mutagenic and carcinogenic effects. [0004] Mycotoxin adsorbent: absorb part, a small amount, and specific toxins physically, and excrete them from the body without changing the properties and chemical structure of the toxins; [0005] At present, the pollution of raw materials in our country is relatively serious, and the pollution of mycotoxins has beco...

AI Technical Summary

Problems solved by technology

However, due to the many types of mycotoxins in this method, the enzyme system is complex and complete, and it is easily affected by the function of the animal's digestive organs; in addition, due to the lack of heat resistance of the enzyme, it is easy to inactivate during the feed processing process, and the cost is high, and it is difficult to Promote the use in actual production

There are many reports on the use of microbial fermentation to remove mycotoxins, such as the fermentation of mycotoxins into other products, but this conversion is usually slow and incomplete, and the safety of the converted products is still inconclusive. To play a role in actual production, systematic and perfect research is needed

Images