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Tea polysaccharide extraction method

An extraction method and technology of tea polysaccharides, which are applied in the field of tea polysaccharide extraction, can solve the problems of high energy consumption, high extraction rate, and loss of tea polysaccharides in microwave extraction, and achieve the goal of maintaining the activity of tea polysaccharides, improving the extraction purity, and increasing the extraction rate Effect

Inactive Publication Date: 2017-03-08
广西钱隆投资管理有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current tea polysaccharide extraction methods mainly include water extraction and alcohol precipitation extraction, ultrasonic extraction, microwave extraction, enzyme extraction, etc. Among them, water extraction and alcohol precipitation extraction can also be divided into hot water alcohol precipitation extraction, cold water alcohol precipitation extraction, acid leaching and extraction. Alkaline leaching method, hot water alcohol precipitation extraction has a higher extraction rate than cold water alcohol precipitation extraction, but Wang Dongfeng and other studies have confirmed that the thermal stability of tea polysaccharides is poor; acid leaching and alkali leaching methods are easy to destroy the structure of polysaccharides , resulting in the loss of tea polysaccharides; ultrasonic extraction will lead to the reduction or inactivation of the activity of tea polysaccharides; high energy consumption and high temperature of microwave extraction; the high cost of enzyme extraction due to the high price of enzymes; the highest extraction rate of existing tea polysaccharides is about 5%

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] A method for extracting tea polysaccharides, which specifically includes the following steps: 1) After pulverizing the tea leaves with liquid nitrogen gas, passing through a 60-mesh sieve, the tea powder is obtained, and the tea powder is soaked in absolute ethanol for 2 hours. The weight ratio of water ethanol to tea powder is preferably 5:1; 2) The ethanol is recovered under reduced pressure. After freezing the tea powder at minus 30°C for 1 hour, it is warmed to 30°C and placed in a radiation field for a dose of 20 kilocuries. The irradiation time is 20 minutes; 3) The irradiated tea powder and water are mixed at a ratio of 1g:20ml and placed in a high-voltage pulsed electric field for extraction, where the electric field strength is 50kv, the number of pulses is 20, and the high-voltage pulsed electric field The extraction time is 30 minutes. The liquid is obtained by filtration. The liquid is placed in a rotary evaporator and concentrated under reduced pressure to 25...

Embodiment 2

[0018] A method for extracting tea polysaccharides, which specifically includes the following steps: 1) After pulverizing the tea leaves with liquid nitrogen gas, passing through a 60-mesh sieve, the tea powder is obtained, and the tea powder is soaked in absolute ethanol for 1 hour. The weight ratio of water ethanol to tea powder is preferably 5:1; 2) The ethanol is recovered under reduced pressure. After freezing the tea powder at minus 20°C for 1 hour, it is warmed to 30°C and placed in a radiation field for a dose of 2 kilocuries. The irradiation time is 10 minutes; 3) The irradiated tea powder and water are mixed at a ratio of 1g:10ml and placed in a high-voltage pulsed electric field for extraction, where the electric field strength is 30kv, the number of pulses is 20, and the high-voltage pulsed electric field The extraction time is 20 minutes. The liquid is filtered. The liquid is put into a rotary evaporator and concentrated under reduced pressure to 25% of the original...

Embodiment 3

[0020] A method for extracting tea polysaccharides, which specifically includes the following steps: 1) After pulverizing the tea leaves with liquid nitrogen gas, passing through a 60-mesh sieve, the tea powder is obtained, and the tea powder is soaked in absolute ethanol for 1 hour. The weight ratio of water ethanol to tea powder is preferably 5:1; 2) The ethanol is recovered under reduced pressure. After freezing the tea powder at minus 20°C for 1 hour, it is warmed to 30°C and placed in a radiation field for a dose of 25 kilocuries. Irradiation, the irradiation time is 30 minutes; 3) Mix the irradiated tea powder and water at a ratio of 1g:10ml and place it in a high-voltage pulsed electric field for extraction, where the electric field strength is 30kv, the number of pulses is 20, and the high-voltage pulsed electric field The extraction time is 20 minutes. The liquid is filtered. The liquid is put into a rotary evaporator and concentrated under reduced pressure to 25% of th...

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Abstract

The invention discloses a tea polysaccharide extraction method, which comprises the step of putting tea powder into a radiation field for radiation. In the process of extracting the tea polysaccharide by the method provided by the invention, the extraction is performed after the tea powder is radiated, so that the extraction rate of tea polysaccharide is 2 to 4 times of the original extraction rate. The extraction rate of the tea polysaccharide is obviously improved; no influence is caused on the activity of the extracted tea polysaccharide. In the optimal scheme, through the high-voltage pulse electric field extraction, the extraction purity of the tea polysaccharide can be improved; before the extraction, absolute ethyl alcohol is used for soaking the tea powder; by using the characteristic that the tea polysaccharide cannot be dissolved in the ethyl alcohol, impurities capable of being dissolved into the ethyl alcohol can be removed through soaking by the ethyl alcohol; the tea polysaccharide purification effect is achieved; after the freezing, the radiation is performed; the radiation efficiency can be improved. In conclusion, the tea polysaccharide extraction method has the general beneficial effects that the extraction rate of the tea polysaccharide and the purity of the tea polysaccharide are improved; the activity of the tea polysaccharide is maintained.

Description

Technical field [0001] The invention relates to the technical field of polysaccharide extraction, in particular to a method for extracting tea polysaccharides. Background technique [0002] Tea polysaccharides are another important physiologically active substance discovered after tea polyphenols. China's research on polysaccharides began in the 1970s and developed rapidly. Due to the diverse biological activity functions of polysaccharides and their extensive use in functional foods and clinical applications, the development, utilization and research of polysaccharide biological resources have become increasingly active, becoming a research hotspot in natural medicine, biochemistry, and life sciences. Tea polysaccharide is the abbreviation of tea compound polysaccharide, composed of sugars, pectin, protein, etc. The polysaccharide part includes arabinose, xylose, glucose, galactose, galactose, and other water-soluble polysaccharides. In recent years, it has been discovered tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/006C08B37/0003
Inventor 黄标黄彬
Owner 广西钱隆投资管理有限公司