Germination-promoting bacillus and application thereof

A technology of Bacillus and Bacillus, which is applied in the field of Bacillus that promotes the growth of Radix Radix, can solve the problems of aggravation of diseases and insect pests, economic loss of pharmaceutical farmers, reduction of yield and quality, etc.

Inactive Publication Date: 2017-03-22
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In response to the above problems, in order to ensure the output of heterophylla heterophylla, it is often necessary to use some chemical fertilizers (such as nitrogen, phosphorus and potassium compound fertilizers) and pesticides (such as carbendazim, kejunkang, tuendazim, guangkuling, and base thiophanate, etc.), and these chemical products will affect the quality of Pseudostellaria Pseudostellariae plantation and Pseudostellariae Pseudostellariae, thereby affecting the environment and clinical drug safety
[0004] At present, a lar

Method used

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  • Germination-promoting bacillus and application thereof
  • Germination-promoting bacillus and application thereof
  • Germination-promoting bacillus and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0083] A kind of bacillus that promotes growth, and described bacillus is isolated and cultivated and obtained like this: specifically comprise the following steps:

[0084] (1) Isolation and screening of autogenous nitrogen-fixing bacteria: Weigh the yellow soil, add 19 times the amount of sterile water, stir for 15 minutes and then let it stand for 10 minutes; take the above supernatant and dilute it to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Soil diluent, then imbibe 10 -3 、10 -4 、10 -5 The soil dilutions were spread on Ashby medium plates with a diameter of 9cm, and each concentration was spread on 3 dishes, and then placed upside down in a constant temperature incubator at 28°C for cultivation. When colonies appeared, the colonies in the repeated dishes were selected and transferred. Put it into a PDA slant test tube, incubate at 25-30°C for 4 days, and set aside;

[0085] (2) Isolation and screening of nitrogen-fixing and potassium-decomposing bacteria: the above-mentio...

Embodiment 2

[0093] A kind of bacillus that promotes growth, and described bacillus is isolated and cultivated and obtained like this: specifically comprise the following steps:

[0094] (1) Isolation and screening of autogenous nitrogen-fixing bacteria: Weigh the mixed soil sample, add 19 times the amount of sterile water, stir for 15 minutes and then let it stand for 10 minutes; take the above supernatant and dilute it to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Soil diluent, then imbibe 10 -3 、10 -4 、10 -5 The soil dilutions were spread on Ashby medium plates with a diameter of 9cm, and each concentration was spread on 3 dishes, and then placed upside down in a constant temperature incubator at 28°C for cultivation. When colonies appeared, the colonies in the repeated dishes were selected and transferred. Into the PDA inclined test tube culture, 4 ℃ constant temperature storage, standby;

[0095] (2) Isolation and screening of nitrogen-fixing and potassium-decomposing bacteria: the abov...

Embodiment 3

[0103] A kind of bacillus that promotes growth, and described bacillus is isolated and cultivated and obtained like this: specifically comprise the following steps:

[0104] (1) Isolation and screening of autogenous nitrogen-fixing bacteria: Weigh the mixed soil sample, add 19 times the amount of sterile water, stir for 15 minutes and then let it stand for 10 minutes; take the above supernatant and dilute to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 Soil diluent, then imbibe 10 -3 、10 -4 、10 -5 The soil dilutions were spread on Ashby medium plates with a diameter of 9cm, and each concentration was spread on 3 dishes, and then placed upside down in a constant temperature incubator at 28°C for cultivation. When colonies appeared, the colonies in the repeated dishes were selected and transferred. Into the PDA inclined test tube culture, 4 ℃ constant temperature storage, standby;

[0105] (2) Isolation and screening of nitrogen-fixing and potassium-decomposing bacteria: the above-m...

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Abstract

The invention discloses a germination-promoting bacillus and an application thereof. The bacillus can be obtained through separating culture: taking a soil sample, employing an Ashby medium, an Alexander silicate bacteria medium, a Mengjinna organophosphorus medium, and a tricalcium phosphate inorganic phosphorus medium, and separating azotobacter, potassium bacteria, organic-phosphorus solubilizing bacteria and inorganic phosphorus solubilizing bacteria. The germination-promoting bacillus has effects for nitrogen fixation, inorganic phosphorus solubilizing, potassium releasing and inhibition of pseudostellaria root root rot fungus, can promote the growth of pseudostellaria root, increases the yield, increases the control effect of the pseudostellaria root root rot, and has promotion effect for content of a root tuber active component saponin. The influence on environment and root tuber quality for plantation of pseudostellaria root by a chemical fertilizer and pesticide cannot be reduced, production investment is not reduced, root tuber output and medicinal value are increased, and the germination-promoting bacillus is in favor of large-area plantation of pseudostellaria root in field.

Description

technical field [0001] The invention relates to a growth-promoting bacillus and application thereof, in particular to a bacillus for promoting the growth of heterophylla. [0002] technical background [0003] Pseudostellaria heterophyla is a commonly used traditional Chinese medicine, which is the dried root of Pscudostaria heterophyla (Miq.). It is a commonly used tonifying medicine in traditional Chinese medicine. It has the effects of nourishing qi and invigorating the spleen, promoting body fluid and moistening the lungs. It can be used for spleen deficiency, fatigue, weakness after illness, spontaneous sweating and thirst, and dry cough due to dryness of the lungs. After Pseudostellaria Pseudostellaria was introduced from Zherong County, Fujian Province, Shibing County, Guizhou Province has developed rapidly and has gradually become one of the economic crops for local farmers to get rid of poverty and become rich. At present, the planting area of ​​Pseudostellaria heter...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00A01G7/06A01G13/00C12R1/125
CPCA01G7/06A01G13/00A01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 王俊丽任建国欧阳桂炉彭洁
Owner GUIZHOU MEDICAL UNIV
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