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Cell non-contact culture method based on micro-fluidic chip

A microfluidic chip and culture method technology, which is applied in the field of non-contact cell culture based on microfluidic chips, can solve the problems of inability to dynamically observe changes in cell behavior, inconvenience in observation and subsequent detection, and difficulty in separating two types of cells. Good application prospects, simple production, and the effect of less sample consumption

Inactive Publication Date: 2017-03-22
SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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AI Technical Summary

Problems solved by technology

Direct contact co-cultivation mixes two or more types of cells according to a certain ratio, but it is difficult to separate the two types of cells, which is not convenient for observation and subsequent detection
Non-direct contact co-cultivation refers to inoculating two or more types of cells on different carriers, and then placing these carriers in the same culture environment, such as the Transwell chamber co-cultivation method, but this method cannot dynamically Observing changes in cell behavior, the amount of cells used is too large to implement fluid control

Method used

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  • Cell non-contact culture method based on micro-fluidic chip
  • Cell non-contact culture method based on micro-fluidic chip
  • Cell non-contact culture method based on micro-fluidic chip

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Embodiment Construction

[0026] As a new type of analysis and detection platform, microfluidic chips have the characteristics of miniaturization, integration, high throughput, low energy consumption, and fast analysis, and have been widely used in many fields. Using the microstructure of the microfluidic chip to simulate the living environment of cells and realize rapid and efficient co-cultivation of multiple cells will become an important platform for cell biology research. The invention uses the microfluidic chip as a platform to establish a non-contact co-cultivation platform for heterogeneous cells, which is combined with electronic equipment such as a microscope to distinguish different types of cells without dyeing and other operations and monitor the biological status of cells under interactive conditions in real time. learning behavior changes. Its micron-scale channel can economically and rationally use resources such as cells and reagents, and is very suitable for cell function research.

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Abstract

The invention discloses a cell non-contact culture method based on a micro-fluidic chip. According to the method, the pressure, which is generated by the height difference between a sample inlet and a sample outlet, is utilized to drive cells into a main channel; due to the laminar flow characteristic of micro fluid, different species of cells are individually fixed on specific areas of the main channel, different cells are not contacted with each other, but in a same culture environment; the cell biological behaviors such as morphologic change of cells, cell migration, and the like, can be directly observed by a microscope; and novel thoughts and a novel technology are provided for the research on the interactions between cells. The provided micro-fluidic chip cell co-culture model realizes the different species cell non-contact co-culture, and has the characteristics of simpleness, practicality, visualization, microminiaturization, low manufacturing cost, and little using amount of reagents and samples.

Description

technical field [0001] The present application belongs to the technical field of cell culture, and in particular relates to a non-contact cell culture method based on a microfluidic chip. Background technique [0002] Cells are the basic unit of the structure and function of organisms. Cells communicate with each other to form a complete individual. The study of intercellular interactions is a research hotspot in today's medical and biological circles, and is the key to exploring the mysteries of life and treating diseases. . [0003] Cell co-cultivation is an important method to study the interaction between cells. Traditional cell co-cultivation methods can be divided into direct contact (mixed) co-culture and non-direct contact co-culture. Direct contact co-cultivation involves mixing and culturing two or more types of cells according to a certain ratio, but it is difficult to separate the two types of cells, which is inconvenient for observation and subsequent detection...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/09C12N5/071C12M3/00C12M1/00
CPCC12M23/16C12N5/0688C12N5/0693
Inventor 刘雯婷聂富强
Owner SUZHOU WENHAO MICROFLUIDIC TECH CO LTD
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