Preparation method and application of live vector vaccine for expressing duck Tembusu virus (DTMUV) prm and E protein recombinant Newcastle disease virus (NDV)

A technology of duck Tembusu virus and Newcastle disease virus, which is applied in the preparation and application of live vector vaccines, can solve the problems of strong virulence and low virus titer, and achieve the effect of reducing detoxification
CN106520710AActive Publication Date: 2017-03-22INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
INST OF ANIMAL HEALTH GUANGDONG ACADEMY OF AGRI SCI
Publication Date
2017-03-22

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Abstract

The invention discloses a preparation method for expressing the duck Tembusu virus (DTMUV) prm and the E protein recombinant Newcastle disease virus (NDV). The preparation method comprises the following steps: 1) constructing full-length plasmids of an attenuated ND GM strain; 2) constructing plasmids for expressing the DTMUV prm and the E protein recombinant NDV; 3) rescuing and identifying the recombinant virus aGM-prm / E. The invention also discloses the virus aGM-prm / E prepared by the method. The virus aGM-prm / E is collected at the China Center for Type Culture Collection (CCTCC), with collection number of CCTCC V201644. A vaccine prepared by utilizing the virus can prevent the ND and the DTMUV disease and conduce to reducing virus removal after virulent NDV infection.
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Description

technical field

[0001] The present invention relates to the preparation and application of a live vector vaccine, in particular to the preparation and application of duck Tembusu virus prm and E protein recombinant Newcastle disease virus live vector vaccine Background technique

[0002] Duck Tembusu virus disease, or duck yellow virus disease, duck hemorrhagic oophoritis, etc., is a new infectious disease that appeared in my country in 2010, and the main victims are ducks and geese. After the onset of laying ducks, the main clinical manifestations are increased body temperature, significantly decreased appetite, and a sharp drop in egg production rate or even complete laying. Symptoms at autopsy mainly manifested as follicular hemorrhage, degeneration, atrophy or dysplasia. The disease incidence rate can reach 100%, and the mortality rate is low. Meat ducks and geese can also get sick, and clinically it is mainly manifested as neurological symptoms, with a death rate of 10...

Claims

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