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Diluent applied to blood analyzer and preparation method thereof

A technology of blood analyzer and diluent, which is applied in the field of diluent for blood analyzer and its preparation, can solve the problems of clinical diagnosis error, poor effect, MCV measurement error, etc., achieve low adsorption, prevent aggregation and The effect of adhesion

Inactive Publication Date: 2017-03-22
刘相东
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, with the prolongation of storage time and the change of temperature, MCV will also change, which will cause errors in the measurement of MCV.
[0007] The existing blood diluents generally add a stabilizer to the diluent formula, so that the pre-diluted blood samples can be left for a period of time to further improve the white blood cell classification characteristics and MCV stability. The effect is not good after a period of time, and the existing diluents for hematology analyzers cannot guarantee MCV, white blood cell classification and stability at the same time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A diluent used in blood analyzers, the composition of which is made of raw materials with the following weights:

[0023] Boric acid 0.8g, borax 0.25g, EDTA-2NA 0.3g, sodium azide 0.02g, anhydrous sodium sulfate 0.8g, sodium chloride 7.0g, deionized water 0.95L, gentamicin 0.08mL.

[0024] A method for preparing the above-mentioned diluent for a blood analyzer adopts the following operation steps:

[0025] a. Take the above parts by weight of boric acid, borax, EDTA-2NA, sodium azide, anhydrous sodium sulfate, sodium chloride, deionized water, and add gentamicin to the reactor in turn at room temperature for full dissolution. mixture;

[0026] b. Inject the mixed solution obtained in step a into a vertical melting glass funnel containing 10 layers of G6 filter membranes for filtration and sterilization to prepare a dilution; the pH of the dilution is 7.8.

[0027] Dilute the diluted solution obtained above.

Embodiment 2

[0029] A diluent used in blood analyzers, the composition of which is made of raw materials with the following weights:

[0030] Boric acid 0.9g, borax 0.28g, EDTA-2NA 0.32g, sodium azide 0.022g, anhydrous sodium sulfate 0.9g, sodium chloride 7.2g, deionized water 0.90L, gentamicin 0.09mL.

[0031] A method for preparing the above-mentioned diluent for a blood analyzer adopts the following operation steps:

[0032] a. Take the above parts by weight of boric acid, borax, EDTA-2NA, sodium azide, anhydrous sodium sulfate, sodium chloride, deionized water, and add gentamicin to the reactor in turn at room temperature for full dissolution. mixture;

[0033] b. Inject the mixed solution obtained in step a into a vertical melting glass funnel containing 10 layers of G6 filter membranes for filtration and sterilization to prepare a dilution; the pH of the dilution is 7.7.

[0034] Dilute the diluted solution obtained above.

Embodiment 3

[0036] A diluent used in blood analyzers, the composition of which is made of raw materials with the following weights:

[0037] Boric acid 1.0g, borax 0.30g, EDTA-2NA 0.35g, sodium azide 0.025g, anhydrous sodium sulfate 1.0g, sodium chloride 7.3g, deionized water 0.90L, gentamicin 0.10mL.

[0038] A method for preparing the above-mentioned diluent for a blood analyzer adopts the following operation steps:

[0039] a. Take the above-mentioned parts by weight of boric acid, borax, EDTA-2NA, sodium azide, anhydrous sodium sulfate, sodium chloride, deionized water, and gentamycin and add them to the reactor in sequence at room temperature to fully dissolve them. mixture;

[0040] b. Inject the mixed solution obtained in step a into a vertical melting glass funnel containing 10 layers of G6 filter membranes for filtration and sterilization to prepare a dilution; the pH of the dilution is 7.82.

[0041] Dilute the diluted solution obtained above.

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Abstract

The invention relates to a diluent applied to a blood analyzer and a preparation method thereof. Per liter of the diluent applied to the blood analyzer comprises the following components: 0.8-1.2g of boric acid, 0.25-0.35g of borax, 0.3-0.4g of EDTA-2NA, 0.02-0.03g of sodium azide, 0.8-1.2g of anhydrous sodium sulfate, 7.0-7.5g of sodium chloride, 0.8-1.2L of deionized water and 0.08-0.12mL of gentamicin. Through the adoption of the scheme, the diluent is used as an isotonic solution; the diluent has a specific conductance value and is used as a special diluent of an electrical impedance method blood cell analyzer; the blood diluted by the diluents effectively blocks synthesis of bacterioprotein and prevents aggregation and adhesion of blood cells; a stable detection environment is supplied to blood cell counting, volume measurement and leukocyte classification.

Description

[0001] Technical field: [0002] The invention relates to a diluent for a blood analyzer and a preparation method thereof. [0003] Background technique: [0004] Blood cell analysis is the most commonly used item in clinical testing. Generally, blood samples are taken from the human body and then analyzed and checked for blood cells in the blood samples, including hemoglobin (HGB), red blood cell count (RBC), white blood cell count (WBC), platelet count (PLT), hematocrit (HCT), average red blood cell volume (MCV), average red blood cell hemoglobin volume (MCH), average red blood cell hemoglobin concentration (MCHC) and other parameters. [0005] When the above parameters are measured on the drawn blood sample, the blood sample body is easy to coagulate during the placement process after the extraction, the cell protein or material components are prone to chemical reaction and decomposition, or the death after in vitro death, etc., the blood sample detection cannot be accurately perfor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N1/38
CPCG01N1/38
Inventor 刘相东
Owner 刘相东
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