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Method and device used for evaluating acute toxicity of solid fumigant on earthworm

An acute toxicity and fumigation device technology, applied in the agricultural field, can solve problems such as the difficulty in the operation of environmental toxicology registration tests, the threat to the safety of test operators, and inaccurate test results, so as to eliminate the accidental death of earthworms, the evaluation results are accurate and reliable, and the operation Easy and fast effect

Active Publication Date: 2017-03-22
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Soil fumigant is a special pesticide with strong volatility and high toxicity. It is very difficult to operate the environmental toxicology registration test. At present, there is a lack of an accurate, reliable, safe and convenient evaluation method
[0003] Although the Organization for Economic Cooperation and Development (OECD), the U.S. Environmental Protection Agency (USEPA) and the Ministry of Environmental Protection of my country have provided standard methods for the acute toxicity of earthworms to conventional pesticides in order to standardize environmental toxicology evaluation tests, but due to the above methods The suggested test device is a glass beaker, and the highly volatile fumigant will escape a large amount during the test, which will cause inaccurate test results and pose a great safety threat to the test operators
Simultaneously, the soil water content suggested in the above method is about 35% of the artificial dry soil weight (that is to say, the saturated water content or the relative water content is 100%). In the experiment, the earthworms treated with blank control will also die in a large number in a short period of time, which leads to the failure of the experiment

Method used

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  • Method and device used for evaluating acute toxicity of solid fumigant on earthworm
  • Method and device used for evaluating acute toxicity of solid fumigant on earthworm
  • Method and device used for evaluating acute toxicity of solid fumigant on earthworm

Examples

Experimental program
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Effect test

Embodiment 1

[0078] Taking Eisenia foetida as an example, the above-mentioned device was used to evaluate the acute toxicity of the 98% solid soil fumigant mass percentage of the Dimenson microparticles to earthworms, as follows:

[0079] 1) Manually adjust the artificial soil air-dried in advance to 75.0% of the saturated water content, weigh 1000g of the above-mentioned artificial soil and place them in a common dryer with a volume of 2.5L;

[0080] 2) Adjust the temperature to 18°C; the light intensity to 400Lx. Put 15 healthy and consistent annular bands into each container, and put them into the obvious young Eisen earthworms;

[0081] 3) After all the earthworms drill into the artificial soil, follow the set gradient concentration of 12.0, 6.0, 3.0, 1.5, 0.75, 0.38, 0.19mg ai / kg 干土 Evenly mix the corresponding quality of Dianlong microgranules (because the actual weighing of the preparation is too low, it is necessary to use quartz sand to dilute the Dianlong preparation 100 times i...

Embodiment 2

[0091] On the basis of Example 1, the inventor adjusted the volume of the fumigation device to 0.3L, the weight of the artificial soil to 100g, and the number of earthworms to 5. The culture temperature is 22°C, the light intensity is 800Lx, and the fumigation time is 3 days. All the other operations are the same as in Example 1.

Embodiment 3

[0093] On the basis of Example 1, the inventor adjusted the volume of the fumigation device to 5L, the weight of the artificial soil to 4000g, and the number of earthworms to 30. The culture temperature is 20°C, the light intensity is 500Lx, and the fumigation time is 21 days. All the other operations are the same as in Example 1.

[0094] Through the verification of Example 2 and Example 3, similar results to Example 1 can also be obtained. In summary, the device and method of the present invention have the advantages of accurate, reliable and repeatable evaluation results, convenient and fast operation, and safety for operators.

[0095] The inventor further provides the following examples to prove the superiority of the method of the present invention.

[0096] Taking Eisenia foetida as an example, the above device was used to evaluate the effects of different artificial soil moisture on the survival of earthworms, as follows:

[0097] 1) Manually adjust the artificial s...

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Abstract

The invention provides a method and a device used for evaluating the acute toxicity of a solid fumigant on earthworm. The method comprises following steps: 1, artificial soil is introduced into a fumigating apparatus; 2, temperature and illumination intensity are adjusted, and a plurality of earthworms are placed on the surface of the artificial soil in step 1; 3, when all the earthworm enter into the artificial soil, the solid fumigant is uniformly mixed with the artificial soil, and is sealed by the fumigating apparatus for fumigating treatment; and 4, death status of the earthworm is investigated, earthworm death rate is calculated, and the acute toxicity of the solid fumigant on earthworm is evaluated based on the earthworm death rate. The method is accurate in evaluation results, high in repeatability, and simple, convenient and rapid in operation, and is safe for operating personnel.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method and a device for evaluating the acute toxicity of a solid fumigant to earthworms. Background technique [0002] In recent years, due to the rapid development of protected cultivation and continuous cropping in my country, the problem of soil-borne diseases has become more and more serious, and the yield and quality of crops have been seriously affected. Soil fumigants are popular with growers because of their thorough disease prevention and significant yield increase, and their usage is increasing year by year. Soil fumigant is a special kind of pesticide with strong volatility and high toxicity. It is very difficult to operate the environmental toxicology registration test. At present, there is a lack of an accurate, reliable, safe and convenient evaluation method. [0003] Although the Organization for Economic Cooperation and Development (OECD), the U...

Claims

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Application Information

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IPC IPC(8): G01N33/50
CPCG01N33/5014
Inventor 毛连纲蒋红云张兰张燕宁任小双王丽萍李哲
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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