Lincomycin detection kit

Abstract

The invention discloses a lincomycin detection kit. The lincomycin detection kit comprises test paper and a micro-porous reagent. The test paper comprises a reaction film, a sample absorption pad, a water absorption pad, a protection film and a bottom board and is formed by adhering the ample absorption pad, the reaction film, the water absorption pad and the protection film on the bottom borad sequentially, wherein the reaction film is provided with a detection area and a quality control area, a lincomycin hapten-carrier protein conjugate wraps the detection area, and an antiantibody wraps the quality control area. Lincomycin specificity antibody-colloidal gold marker is freeze-dried on the micro-porous reagent, and the micro-porous reagent is provided with micro-porous plugs. The lincomycin detection kit has the advantages of wide linear dynamics range, long optical signal duration time, simplicity and rapidity in analysis method, stable results, small errors, high safety and long service life. Through chemiluminescence immunoassay, the lincomycin detection kit succeeds in detecting substances which cannot be detected by the methods such as radioimmunoassay and enzyme-linked immunoassay, thereby being of great significance to early clinical diagnosis.

Description

technical field [0001] The invention relates to a kit, in particular to a lincomycin detection kit. Background technique [0002] At present, the techniques for detecting lincomycin antibiotic residues in milk mainly include the following methods: (1) Microbial inhibition test, also known as microbiological inhibition test (MIT for short), according to the physiological function, reproduction and metabolism of specific microorganisms by antibiotics It is a classic and widely used method to detect antibiotics in milk to qualitatively or quantitatively determine the amount of antimicrobial drug residues in the sample through the inhibitory effect of the method. my country's standard for the detection of antibiotic residues in fresh milk (GB4789.27-2003), the antibacterial zone test and turbidity test commonly used abroad in the 1970s and 1980s, and the discoloration detection kits currently on the market are all such. Different types of indicator bacteria have different sensi...

AI Technical Summary

Problems solved by technology

However, the microbiological detection operation is time-consuming and laborious, and is not suitable for large-scale production

(2) Physical and chemical detection methods After the 1990s, most physical and chemical detection methods for the determination of lincomycin residues in food mainly rely on liquid chromatography for separation, followed by chromatography / mass spectrometry, gas chromatography , high-performance thin-layer chromatography and other detection methods, because of their unique performance, are slightly applied in the detection of antibiotic residues

However, because antibiotics are haptens, the antigen construction technology is difficult, the immune specificity is strong, and the detection cost is high. At present, IDEXX Laboratory Company, Chahu Sciences and other companies have relatively mature research on the free analysis method, and this technology is still in research and development in China. middle

At present, enzyme-linked immunosorbent assay, radioimmunoluminescence assay, fluorescence immunoassay, chemiluminescence method, etc. are mainly used. Comparing the sensitivity of several marker immunological techniques shows that enzyme immunoassay has a lower sensitivity than luminescence.