Preparation method of galactose type salidroside and derivative thereof

Abstract

The invention relates to a preparation method of galactose type salidroside and a derivative thereof. The method comprises the following steps that 1, a reaction system with the lactose concentration being 0.1-1 M, the p-hydroxyphenylethyl alcohol concentration being 0.05-0.5 M and the beta-galactosidase concentration being 0.004-4 mg / mL is prepared a phosphate buffer; 2, the reaction system reacts for 5-30 min under the condition that the temperature ranges from 30 DEG C to 50 DEG C, a reaction is terminated, solid-liquid separation is conducted, and supernatant is taken; 3, the supernatant is separated and dried, and the galactose type salidroside and the derivative thereof are prepared. According to the method, a substrate is cheap, reacting is rapid, operation is easy and convenient, time and labor are saved, the production cost is low, and the application prospect is wide.

Description

technical field

[0001] The invention relates to a preparation method of galactosyl-type salidroside and its derivatives, in particular to a method for synthesizing p-hydroxyphenylethylgalactoside and its derivatives by using β-galactosidase, belonging to the technical field of sugar engineering .

[0002] technical background

[0003] Salidroside naturally exists in the medicinal plant Rhodiola rosea, and its chemical structure is p-hydroxyphenylethyl-O-β-D-glucoside, which has important pharmacological functions, such as neuroprotection, cardiovascular protection, delaying Anti-aging, anti-fatigue, anti-oxidation, anti-tumor, anti-coagulation, anti-virus, anti-microwave radiation, etc., are added in a small amount in traditional Chinese medicine and skin care products, and have great application potential. At present, it has been found that the galactoside-type salidroside, that is, p-hydroxyphenylethyl-β-D-galactoside, is superior to salidroside in terms of neuroprotection...

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