Kit for detection of liver function glycyl-proline dipeptidyl aminopeptidase and preparation method

Abstract

The invention discloses a kit for detection of liver function glycyl-proline dipeptidyl aminopeptidase and a preparation method. The kit includes a reagent A and a reagent B, wherein the reagent A comprises, by weight, 20-25 parts of a buffer solution, 16-19 parts of glycylglycine, 14-18 parts of sodium azide, 8-12 parts of a stabilizing agent, and 30-35 parts of deionized water; the reagent B includes, by weight, 21-25 parts of glycyl-proline p-nitroaniline p-toluenesulfonic acid, 15-19 parts of sodium azide, 19-23 parts of a buffer solution, 11-15 parts of a stabilizing agent, 22-26 parts of ethylene glycol, 6-9 parts of a surfactant, and 31-34 parts of deionized water; the buffer solution includes, by weight, 15-20 parts of trihydroxymethyl aminomethane, 20-25 parts of deionized water, and 18-22 parts of hydrochloric acid. A preparation method includes the steps of: mixing the trihydroxymethyl aminomethane and the deionized water. The kit has excellent stability and can improve precision and accuracy of detection and satisfy a strict demand. The kit is enlarged in use range, has simple preparation method and is convenient to use, and is low in cost.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a liver function glycylproline dipeptide aminopeptidase assay kit and a preparation method. Background technique [0002] As early as 1966, Hopsu-Havu and Glenner found that in the biochemical and histochemical studies of mammalian peptidases, the chromogenic substrate glycyl-prolyl-β-naphthylamine could be hydrolyzed by certain enzyme preparations, they The enzyme that can hydrolyze Gly-Pro-β-NA was successfully isolated from these enzyme preparations, and its hydrolysis products were proved to be glycyl-proline and β-naphthylamine; in order to explore whether there is This enzyme, they first studied the liver of mice and found that this enzyme does exist; then, they incubated the kidney slices of defatted mice with Gly-Pro-β-NA and azo dyes, and found that small There is naphthylamine produced by hydrolysis in the kidney tissue of mice, indicating that this ...

AI Technical Summary

Problems solved by technology

[0004] The patent document with the application number 201510973109.8 discloses a glycylproline dipeptide aminopeptidase detection kit. Compared with conventional kits, the kit has better stability and linear range than conventional detection kits. It is beneficial to the promotion and application of reagents in clinic; however, its stability does not meet the strict requirements, resulting in a limited range of use, and low precision and accuracy. Therefore, we propose a liver function glycylproline Acid dipeptide aminopeptidase assay kit and preparation method are used to solve the above problems