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Preparation method of chloramphenicol compounds

A technology of chloramphenicol and compounds, which is applied in the fields of biopharmaceuticals and biochemical industries, can solve the problems of harsh reaction conditions, low atom economy, unfriendly environment, etc., and achieve the effects of simple operation, simple post-treatment, and less three wastes

Active Publication Date: 2017-04-19
SUZHOU LEAD BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The technical problem to be solved by the present invention is that the process for preparing chloramphenicol in the prior art has high production cost, long reaction steps, harsh reaction conditions, low atom economy, and unfriendly problems to the environment, and then provides a low-cost, A method for preparing chloramphenicol that is easy to prepare, high in yield and more environmentally friendly

Method used

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  • Preparation method of chloramphenicol compounds
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  • Preparation method of chloramphenicol compounds

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 9

[0046] Example 9 is the preparation of (R, R)-thrao-type-1-p-nitrophenyl-2-amino-1,3-propanediol (D-amino compound) Example 10 is a method for preparing chloramphenicol using the D-amino compound prepared in Example 9, and the synthetic route is:

[0047]

Embodiment 1

[0051] The structure of the chloramphenicol intermediate used in this embodiment is as follows:

[0052]

[0053] Take 1g of chloramphenicol intermediate and 1.25g of glucose in a 100mL three-necked flask, and then add 50mL of PBS buffer with a pH of 6.5 and a concentration of 0.05mol / L; put the three-necked flask into the reaction pot, Set the speed to 850rpm, the temperature is 30℃, and then add 5mg NADP respectively + , 20mg glucose dehydrogenase (purchased from Suzhou Yinhang Biological Technology Co., Ltd., the product number is YH1901, only one of the models is given here to illustrate the effect of the present invention, and the various types of products on the market are useful for realizing the present invention. There is no difference in terms of the purpose, the same below, no further details will be given below), and 30mg ketoreductase powder (purchased from Suzhou Yinhang Biotechnology Co., Ltd.) to obtain a mixed solution, and the temperature of the mixed solution is...

Embodiment 2

[0060] The structure of the chloramphenicol intermediate used in this embodiment is as follows:

[0061]

[0062] Take 5g of chloramphenicol intermediate and 6.25g of glucose in a 100mL three-necked flask, add 50mL of PBS buffer with a pH of 6.5 and a concentration of 0.05mol / L; put the three-necked flask into the reaction pot, set the speed at 850rpm and temperature 30℃, then add 5mg NADP + , 100mg glucose dehydrogenase, and 100mg ketoreductase powder (purchased from Suzhou Yinhang Biotechnology Co., Ltd., the product number is YH2047, only one of the models is given here to illustrate the effect of the present invention, and each model is commercially available There is no difference between the products for achieving the purpose of the present invention, the same below, and will not be repeated hereafter), to obtain a mixed solution, and make the mixed solution react at a temperature of 30°C, and at the same time, use 2mol / L of NaOH solution to maintain the pH value of the mi...

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Abstract

The invention provides a preparation method of chloramphenicol compounds. The preparation method comprises the following steps: adding a chloramphenicol intermediate product to a buffer solution; then, adding ketoreductase to the solution so as to obtain a mixed solution; and conducting a reaction on the mixed solution at 25-35 DEG C, so that the chloramphenicol compounds are obtained. The preparation method of the chloramphenicol compounds provided by the invention not only reduces raw material cost but also improves economy of raw materials and improves yield; and the problems of the prior art that isomer by-products, obtained from the preparation of chloramphenicol, cannot be used, raw material cost is high and yield is low are solved.

Description

Technical field [0001] The invention relates to a preparation method of chloramphenicol compounds, belonging to the technical field of biopharmaceuticals and biochemicals. Background technique [0002] Chloramphenicol is an antibiotic produced by Zipperaria Venezuela. Its antibacterial mechanism is to bind to the nucleosome 50S subunit to inhibit peptide acyltransferase, thereby inhibiting protein synthesis. Its structural formula is as follows: [0003] [0004] Chloramphenicol is a broad-spectrum antibiotic with small side effects and high efficacy. It is effective against most Gram-negative and positive bacteria, especially typhoid fever and paratyphoid bacillus; it has the strongest effect on influenza bacillus, pertussis and dysentery The effect is also strong; it also has an inhibitory effect on Escherichia coli, pneumoniae, Proteus and Pseudomonas aeruginosa; it is also effective against Rickettsia and Chlamydia trachomatis. [0005] Due to the wide range of uses of chloramph...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02
CPCC12P13/02
Inventor 谢新开黄晓飞张金鑫张瑞杰
Owner SUZHOU LEAD BIOTECH CO LTD
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